Identification of Sour Orange Accessions and Evaluation of Their Genetic Variability by Molecular Marker Analyses

Siragusa, Maria Gabriella; Pasquale, Fabio De; Abbate, Loredana; Tusa, N.

doi:10.21273/hortsci.41.1.84

Cited by 25 publications

(19 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ISSR markers technique has been known as a rapid, reproducible and useful method for distinguishing among different cultivars and clustering genotypes in the citrus species (Siragusa et al, 2006;Yang et al, 2010). In the present study, 13 primers produced clear, species-specific fingerprint patterns with all samples and were sufficient to discriminate varietal groups of citrus and produced results consistent with previous studies (Yang et al, 2010).…”

Section: Genetic Analysis Of the Citrus Genussupporting

confidence: 86%

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Youseif¹,

Halwagi²,

Sayed³

et al. 2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Citrus species are among the most important fruit trees in the world and are considered as a major export product of Egypt. Forty-eight Citrus L. accessions representing six citrus groups (orange, mandarin, lemon, sour orange, grape fruit and pummelo) were collected. Chemical proprieties including pH, total acidity, total soluble solids and ascorbic acid of different fruit juices were determined. Eight accessions representing different citrus groups were screened for their antibacterial activity against five pathogenic bacteria (Escherichia coli, Salmonella typhi, Staphylococcus aureus, Micrococcus spp. and Bacillus pumilus). Lemon and lime accessions exhibited the highest antibacterial activity compared to the standard antibiotics (ampicillin and streptomycin). However, grapefruit and pummelo accessions showed no inhibitory effect. Inter-simple sequence repeats (ISSR) markers were used to study the genetic diversity and phylogenetic relationships among citrus accessions. The highest level of polymorphism (71%) was detected amongst lemon and lime accessions, whereas, the lowest percentage of polymorphism (18%) was identified within the sour orange group. The phylogenetic tree separated the varieties into discrete clusters according to their respective citrus group. Citrus groups were initially divided into two main clusters at 0.18 level of similarity. Lemon, lime, mandarin and sour orange were grouped in the first cluster, while sweet orange, grapefruit and pummelo were nested in the second cluster.

show abstract

Section: Genetic Analysis Of the Citrus Genussupporting

confidence: 86%

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Youseif¹,

Halwagi²,

Sayed³

et al. 2014

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…On the other hand, the present result suggested that the rates of seedlings generating adventitious shoots in the cut end of epicotyls is genotype-specific, although the rates were different in three sour orange cultivars 'Kabusu', 'Zadaidai', and 'Variegated Daidai' (Table 2). Sour orange cultivars have been reported to be either genetically identical clones or sour orange hybrids (Siragusa et al, 2006). In the present study, the rates of decapitated seedlings generating adventitious shoots on the cut edge increased with increases in the length of epicotyls after decapitation.…”

Section: Discussionsupporting

confidence: 49%

Evaluation of Seedlings from 11 <i>Citrus</i> Accessions for <i>In Vivo</i> Micrografting

Sakai

Mizunoe

et al. 2020

Hortic J

View full text Add to dashboard Cite

The very young seedlings of trifoliate orange, and its hybrids with trifoliate leaves as a marker, are usually used as a rootstock for in vitro and in vivo micrografting of citrus to eliminate viruses in spring in temperate regions. In tropical and subtropical regions or in summer, however, the production and use of trifoliate orange seedlings is difficult. Therefore, it is necessary to establish suitable micrografting using efficient Citrus seedlings adapted to these regions and summer. In decapitated seedlings for micrografting, adventitious shoots on the cut end of the epicotyls and shoots from cotyledon axillary buds are often formed. Hence, the potential for adventitious shoot formation and cotyledon axillary shoot formation was firstly studied with decapitated seedlings from 11 Citrus accessions and one Poncirus accession as a control. Mature seeds of the 12 accessions were germinated in vivo and seedlings of various ages (2-, 4-, and 8-week-old, 4-month-old and 8-month-old after germination) were decapitated at three (lower, middle, and upper) positions on the epicotyls. Adventitious shoot formation decreased with increases in the age of seedlings decapitated at eight weeks or 4 months after germination. The percentage of decapitated seedlings forming adventitious shoots was different in different accessions ranging from 0% to 100%, and increased with increases in decapitation height in the epicotyls. It was estimated from these results that 2-week-old seedlings of Natsudaidai, Shiikuwasha, 'Hiradobuntan', 'Variegated Daidai', and trifoliate orange 'Flying Dragon' had higher potential to support the initial growth of adventitious shoots, and cotyledon axillary shoots than the others, and that decapitation at the upper one-third and middle of epicotyls resulted in higher adventitious shoot formation than the lower one-third. In in vivo micrografting of satsuma mandarin on the seedlings of these accessions, Natsudaidai, 'Hirado-buntan', and 'Variegated Daidai' seedlings resulted in high micrografting success rates, whereas 'Kabusu', 'Flying Dragon' and Shiikuwasha seedlings resulted in very low success rates. The success rate decreased with increases in seedling age. It has become clear from these results that there is no relationship between the potential for shoot formation and micrografting success and that the 2-week-old seedlings of Natsudaidai, 'Hirado-buntan' and 'Variegated Daidai' are efficient rootstocks for micrografting.

show abstract

“…'Goutou' and 'Zhuluan' were probably sour orange-pummelo hybrids used as rootstocks introduced from China, [39][40][41] and they were closely clustered with the true sour orange 'Sour' based on the SNP genotypic data, as well as fruit characteristics ( Fig. 4; see also Supporting information, Fig.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes

et al. 2017

View full text Add to dashboard Cite

The clustering analysis based on the aroma volatile compositions was able to differentiate mandarin varieties and natural sub-groups, and was also supported by the molecular marker study. The gas chromatography-mass spectrometry analysis of citrus juice aroma volatiles can be used as a tool to distinguish citrus genotypes and assist in the assessment of future citrus breeding programs. The aroma volatile profiles of the different citrus genotypes and inter-relationships detected among volatile compounds and among citrus genotypes will provide fundamental information on the development of marker-assisted selection in citrus breeding. © 2017 Society of Chemical Industry.

show abstract

Identification of Sour Orange Accessions and Evaluation of Their Genetic Variability by Molecular Marker Analyses

Cited by 25 publications

References 42 publications

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Chemical analyses, antibacterial activity and genetic diversity assessment of some Egyptian Citrus spp. cultivars

Evaluation of Seedlings from 11 <i>Citrus</i> Accessions for <i>In Vivo</i> Micrografting

Comparative analysis of juice volatiles in selected mandarins, mandarin relatives and other citrus genotypes

Contact Info

Product

Resources

About