Identification of SSR markers for hybridity and seed genetic purity testing in sunflower (Helianthus annuus L.)

Abstract

The genuineness of a hybrid is one of the most important characteristics of good quality seed. In order to identify pure hybrid and pollen shedders/ offtypes an investigation was performed to identify an ideal SSR marker. 58 primer pairs were screened to identify the specific marker associated with each hybrid and parental lines. Hybrid KBSH-44 could be clearly identified by using ORS 309 and ORS 170, based on the banding pattern resolved on polyacrylamide gel (6%). The complementary banding pattern of both parents made a way to identify the hybrid. ORS 309 amplified allele size at 250 bp was specific to female parent (CMS-17A) and 230 bp was specific to male parent (RHA 95-C-1). These two bands of allele size 230 and 250 bp were found only in hybrid KBSH-44. Another SSR primer ORS 170 was able to distinguish the hybrid KBSH-44 by amplifying allele of size 230 bp a female specific (CMS-17A) allele and 200 bp amplicon a male specific allele (RHA 95-C-1). SSR primer ORS 811 found specific to identify KBSH-53 and it amplified allele of size 270 bp in its female parent (CMS-53A) and allele size of 230 bp in its pollen parent (RHA 95-C-1). The hybrid has both the alleles from its parents at 270 and 230 bp.

Storage stability of value added products from sunflower kernels

“…Recently attempts to grow sunflower kernels for preparing products are being made (Shadakshari, Y. G. Geneticist, AICRP Sunflower, GKVK, UAS, Bengaluru, Personnel communication 2010). Several cultivars such as KBSH 1, KBSH 41, KBSH 42, KBSH 44, KBSH 53, Surajmukhi etc., (Pallavi et al 2011) are available. Screening some of varieties showed that KBSH 44 was having a good hullability (Muttagi, GKVK, UAS, Bengalu, unpublished work, 2009).…”

Utilization of SSR Markers for Seed Purity Testing in Popular Maize Hybrids

“…The SSR markers identified had both female and male specific bands and are useful in genetic purity testing. The use of SSR markers for genetic purity testing has already been demonstrated in maize (Daniel et al, 2012;Mrutu, 2015;Simon and Lovasz, 2016;Wu et al, 2010); rice (Bora et al, 2016;Galal et al, 2014;Moorthy et al, 2011;Sudharani et al, 2013), pearl millet (Nagawade et al, 2016), eggplant (Reddy et al, 2015), soybean (Zhang et al, 2014), cauliflower (Zhao et al, 2012) and in sunflower (Pallavi et al, 2011).…”

“…Among available DNA markers systems, PCR based co-dominant SSRs (also known commonly as microsatellites) are preferred for genotyping because of their reproducibility, abundance and amenability to high throughput screening. The SSR markers are of great importance for rapid assessment of hybrid and parental line seed purity (Dongre et al, 2011(Dongre et al, , 2012Pallavi et al, 2011;Reddy et al, 2011Reddy et al, , 2015. These markers allow the early identification of true interspecific hybrids for further evaluation and crossing, and simultaneously, enable the early disposal of non-hybrids, thus delivering substantial savings in time and resources.…”

Genetic purity assessment of indica rice hybrids through DNA fingerprinting and grow out test

“…These results are in close agreement with Yoshitola (2002). Since STMS and STS markers cover whole genome, they show polymorphism in DNA banding pattern of different genotypes which reveal the variation and allelism (marker alleles) in larger quantity (Yashitola , 2002;Nandkumar , 2004;Sayyed , 2009;Antonova , 2006;Sundaram , 2008 ;Jaikishan , 2010;Pallavi , 2011Kumar , 2012Vikas 2014;Kumar 2016).…”