Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

On, Stephen L. W.

doi:10.1016/s0378-1097(00)00475-4

Cited by 46 publications

(89 citation statements)

References 14 publications

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“…In a recent study by Duim et al (2004), several groups were discerned within this species. A previous study based on numerical analysis of AFLP profiles demonstrated that classical urease-negative and ureasepositive strains form closely related, but distinct, clusters (On & Harrington, 2000). Our results confirm that C. lari is a heterogeneous group as demonstrated in both the rpoB and 16S rRNA gene-derived trees.…”

supporting

confidence: 87%

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak

Stieber

Emler

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.The genus Campylobacter was first proposed by M. Sebald and M. Véron in 1963 and included only the type species Campylobacter fetus and Campylobacter bubulus, now known as Campylobacter sputorum (Sebald & Véron, 1963). These taxa had formerly been classified as Vibrio species. In 1973, M. Véron and R. Chatelain included several misclassified Vibrio in the distinct Campylobacter genus based on serological, biochemical and DNA-DNA hybridization investigations (On, 2001). Since then, the taxonomy of the genus has changed dramatically. At present, it comprises 17 species with validly published names and six recognized subspecies (On, 2001;Foster et al., 2004). In addition, strains belonging to C. sputorum are divided into three biovars, sputorum, fecalis and paraureolyticus, on the basis of their ability to produce catalase or urease Vandamme & On, 2001).In general, members of the genus Campylobacter colonize the mucosal surfaces of the intestinal tract, oral cavity or urogenital tract of healthy, as well as diseased, humans and animals, especially birds. Several species may act as pathogens, causing disease in both human and animal hosts. Twelve of the 17 Campylobacter species are associated with human diseases. Campylobacter jejuni and Campylobacter coli are particularly frequent causative agents of human bacterial intestinal disorders worldwide (Skirrow, 1994). There have also been reported cases of diarrhoea in man caused by Campylobacter upsaliensis and Campylobacter lari, but the frequency of these infections is very low (Bourke et al., 1998;Van Doorn et al., 1998). Occasionally, Campylobacter species are implicated as causative agents of pericard...

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supporting

confidence: 87%

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Korczak

Stieber

Emler

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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“…Recently, this method has been adapted for epidemiological typing of C. jejuni and C. coli. These studies demonstrated the subtyping of individual strains, and have already differentiated between two species (Duim et al, , 2000Kokotovic & On, 1999 ;On & Harrington, 2000). In this study, the value of AFLP analysis was determined for the identification and differentiation, at strain level, of nine Campylobacter species and three subspecies belonging to well-defined taxa and representing Campylobacter species that are very important in a veterinary context.…”

Section: Introductionmentioning

confidence: 93%

Differentiation of Campylobacter species by AFLP fingerprinting

Duim¹,

Vandamme

Rigter³

et al. 2001

Microbiology

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The fluorescent amplified fragment length polymorphism (AFLP) fingerprinting method was tested for its ability to identify and subtype the most important Campylobacter species found in veterinary infections. Sixty-nine reference strains and 19 clinical isolates of Campylobacter jejuni subsp. jejuni, Campylobacter jejuni subsp. doylei, Campylobacter upsaliensis, Campylobacter coli, Campylobacter lari, Campylobacter fetus subsp. fetus, C. fetus subsp. venerealis, Campylobacter hyointestinalis subsp. hyointestinalis, C. hyointestinalis subsp. lawsonii, Campylobacter mucosalis, Campylobacter helveticus and Campylobacter sputorum were subjected to analysis. The topology of the dendrogram obtained by numerical analysis of the AFLP profiles did not reflect the phylogenetic relationships as derived from 16S rDNA sequence comparison. However, except for C. lari, AFLP analysis grouped the strains that belonged to the same genomic species into distinct clusters. C. lari strains were separated into two distinct AFLP groups, which corresponded with nalidixic-acid-sensitive and -resistant variants of C. lari. These results correlated with data from whole-cell protein profiling. Within C. jejuni, C. hyointestinalis and C. fetus, strains could be identified at the subspecies level. AFLP analysis also allowed the subtyping of most species at the strain level. It is concluded that AFLP analysis is a valuable tool for concurrent identification of campylobacters at the species, subspecies and strain levels. In addition, the data confirm and extend previous reports showing that C. lari is a heterogeneous species that may comprise multiple taxa.

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“…When On and Harrington studied the taxonomic and epidemiological relationship among Campylobacter species by numerical analysis of amplified fragment length polymorphism (AFLP), a high level of genetic diversity in C. lari, particularly amongst UPTC isolates, was identified [117]. Multilocus enzyme electrophoresis analysis, which were shown to be the most successful at discriminating UPTC organisms at the subspecies level, whereas serotyping, phage-typing, antibiogram typing and flagellin typing were unsuccessful [101], also demonstrated that the UPTC isolates (n = 31) isolated from several countries and sources examined are genetically hypervariable and form a cluster separate from the C. lari (n = 3) cluster [94].…”

Section: Atypical Campylobactersmentioning

confidence: 99%

Campylobacter

et al. 2005

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-Species within the genus, Campylobacter, have emerged over the last three decades as significant clinical pathogens, particularly of human public health concern, where the majority of acute bacterial enteritis in the Western world is due to these organisms. Of particular concern are the species, C. jejuni and C. coli, which are responsible for most of these gastrointestinal-related infections. Although these organisms have already emerged as causative agents of zoonoses, several aspects of their epidemiology and pathophysiology are only beginning to emerge. Trends in increasing antibiotic resistance are beginning to emerge with oral antibiotics, which may be the drug of choice for when it is necessary to intervene chemotherapeutically. This review wishes to examine (i) emerging clinical aspects of the disease, such as Guillain Barré syndrome (GBS), (ii) the association between these organisms and poultry as a natural host, (iii) environmental aspects of Campylobacter epidemiology, (iv) the emergence of atypical campylobacters (v) emerging trends in antibiotic resistance, (vi) adoption of modern methods for the detection of campylobacters.

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Identification of taxonomic and epidemiological relationships among Campylobacter species by numerical analysis of AFLP profiles

Cited by 46 publications

References 14 publications

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Genetic relatedness within the genus Campylobacter inferred from rpoB sequences

Differentiation of Campylobacter species by AFLP fingerprinting

Campylobacter

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