Identification of the clpB and bipA genes and an evaluation of their expression as related to intracellular survival for the bacterial pathogen Piscirickettsia salmonis

Isla, Adolfo; Haussmann, Denise; Vera, Tamara; Kausel, Gudrun; Figueroa, Jaime

doi:10.1016/j.vetmic.2014.08.014

Cited by 25 publications

(19 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found similar results in our study, i.e. the clpB gene was significantly more expressed in the CC medium (Sf21 cell line), thereby confirming the hypothesis of Isla et al [15]. Despite the fact that phenotypic protein expressions have not yet have been demonstrated in P .…”

Section: Discussionsupporting

confidence: 92%

“…The presence of the clpB gene has been described in the P . salmonis showing significantly increased expression during infection of the SHK-1 cell line compared to the cell-free medium, indicating that clpB could also be expressed during propagation in host cells permitting the adaptation to the hostile conditions in the host [15]. We found similar results in our study, i.e.…”

Section: Discussionsupporting

confidence: 88%

“…A recent experiment using the SHK-1 line, suggested that P . salmonis significantly increased the gene expression of two homologues of the clpB and bipA , when compared with a cell free media [15]. These genes show increased expression allowing the pathogen to evade the cellular immune response and replicate inside macrophages [15].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism

Machuca

Martı́nez

2016

PLoS ONE

View full text Add to dashboard Cite

The intracellular facultative bacteria Piscirickettsia salmonis is one of the most important pathogens of the Chilean aquaculture. However, there is a lack of information regarding the whole genomic transcriptional response according to different extracellular environments. We used next generation sequencing (NGS) of RNA (RNA-seq) to study the whole transcriptome of an isolate of P. salmonis (FAVET-INBIOGEN) using a cell line culture and a modified cell-free liquid medium, with or without iron supplementation. This was done in order to obtain information about the factors there are involved in virulence and iron acquisition. First, the isolate was grown in the Sf21 cell line; then, the bacteria were cultured into a cell-free liquid medium supplemented or not with iron. We identified in the transcriptome, genes associated with type IV secretion systems, genes related to flagellar structure assembly, several proteases and sigma factors, and genes related to the development of drug resistance. Additionally, we identified for the first time several iron-metabolism associated genes including at least two iron uptake pathways (ferrous iron and ferric iron uptake) that are actually expressed in the different conditions analyzed. We further describe putative genes that are related with the use and storage of iron in the bacteria, which have not been previously described. Several sets of genes related to virulence were expressed in both the cell line and cell-free culture media (for example those related to flagellar structure; such as basal body, MS-ring, C-ring, proximal and distal rod, and filament), which may play roles in other basic processes rather than been restricted to virulence.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism

Machuca

Martı́nez

2016

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…This intracellular facultative bacterium is responsible for massive mortalities in the three salmonid species farmed in Chile, accounting for 90% of all industry-related antibiotic use in Chile over recent years (Otterlei et al, 2016). Existing published studies for this bacterium have described phenotypic traits, virulence factors, and pathogenicity, in addition to determining whether P. salmonis possesses a mechanism for antibiotic resistance (Cartes et al, 2017;Isla, Haussmann, Vera, Kausel, & Figueroa, 2014;Otterlei et al, 2016). Nevertheless, studies evaluating this pathogenic bacterium from an evolutionary and genetic standpoint are scarce (Otterlei et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure

Isla

Saldarriaga‐Córdoba

Fuentes

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic‐resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty‐two Chilean P. salmonisisolates, as well as the type strain LF‐89T, were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF‐89T and EM‐90 strains, as well as a seven‐isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen.

show abstract

“…The virulence factor BipA/TypA and the heat shock protein ClpB are two such widely characterized proteins. Isla et al (2014) reported the complete coding sequence of the genes clpB and bipA from P. salmonis. Moreover, the experimental results suggest that during SHK-1 infection, the pathogen significantly increases the expression of the proteins ClpB and BipA.…”

Section: P Salmonis: Virulence and Pathogenicitymentioning

confidence: 99%

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Figueroa

Cárcamo

Yáñez

et al. 2019

Reviews in Aquaculture

View full text Add to dashboard Cite

The rapid growth of the salmon farming industry in Chile has led to the appearance of various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. The Chilean salmon industry has suffered several health crises associated with high fish mortalities, such as caused by the infectious salmon anaemia virus (2007) and harmful algal blooms (2016). In addition to these events, marine farms are continuously affected by outbreaks of harmful pathogens, including the bacteria Piscirickettsia salmonis and, most recently, a reappearance of Renibacterium salmoninarum, and the infectious pancreatic necrosis virus. These outbreaks have led to stricter regulations, but the salmon farming industry continues to struggle despite reforms. In addition to the aforementioned pathogens, which are a continuous threat, other apparently under control pathogens have also reappeared in recent years. In this review, we analyse the current state of knowledge on four of the main pathogens affecting salmon farming in Chile. The infectious pancreatic necrosis virus is relevant as it affects freshwater‐stage fish, with survivors then acting as carriers. The infectious salmon anaemia virus currently appears to be under control; however, P. salmonis and R. salmoninarum continue to be the cause for high mortalities in the Chilean aquaculture industry.

show abstract

Identification of the clpB and bipA genes and an evaluation of their expression as related to intracellular survival for the bacterial pathogen Piscirickettsia salmonis

Cited by 25 publications

References 20 publications

Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism

Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism

Multilocus sequence typing detects new Piscirickettsia salmonis hybrid genogroup in Chilean fish farms: Evidence for genetic diversity and population structure

Addressing viral and bacterial threats to salmon farming in Chile: historical contexts and perspectives for management and control

Contact Info

Product

Resources

About