Identification of the first major allergen of a squid (Todarodes pacificus)

Identification of the major allergen of Macrobrachium rosenbergii (giant freshwater prawn)

“…In addition, tropomyosin has also been identified as the major allergen of other crustaceans [9,10] , mollusks [11][12][13][14][15] , house dust mites [16,17] and cockroaches [18,19] .…”

Reduced Allergenic Potency of VR9-1, a Mutant of the Major Shrimp Allergen Pen a 1 (Tropomyosin)

“…Pen a 1 inhibited at least 75% of patients' IgE radioallergosorbent test reactivity to whole body shrimp extract, indicating that Pen a 1 is also responsible for most of the allergenic activity of shrimp (2). Tropomyosin is a major allergen in other Crustacea species (3)(4)(5)(6) and in dust mite (7)(8)(9)(10)(11), cockroach (12,13), lobster (4), squid (14), and other mollusks (15)(16)(17)(18)(19), suggesting that tropomyosin may be regarded as an invertebrate pan-allergen (20). Indeed, cross-reactivity between cockroaches and mites with Crustacea was attributed to tropomyosin (4,21,22).…”

Extraction of Squid (Photololigo Duvaucelii) Myofibrillar and Sarcoplasmic Proteins to Create A Skin Prick Test Reagent In The Diagnosis of Food Allergy

“…Specific IgE antibodies in serum 12 that could bind to squid myofibrillar protein were 40.0 kDa and 35.9 kDa, whereas in respondent serum 15 were 83.9 kDa and 78.7 kDa and in respondent 20 were 87,6 kDa, 77,8 kDa, 61,0 kDa, 35,1 kDa, 22,1 kDa, 11,0 kDa, and 9,9 kDa. The 38.6 kDa protein which was detected using serum 1 was suspected as a major allergen contained in the squid, namely tropomyosin proteins (Tod p 1) according to Allergen Nomenclature ( [9]; [17]; [18]). Other major allergen found in squid is tropomyosin protein with the molecular weight of 36 kDa and the one that could be detected using serum 12 has 35.9 kDa molecular weight [19].…”