Identification of TIFY Family Genes and Analysis of Their Expression Profiles in Response to Phytohormone Treatments and Melampsora larici-populina Infection in Poplar

Abstract

The TIFY domain contains approximately 36 conserved amino acids that form the core motif TIF[F/Y]XG, and they were reported to play important roles in plant growth, tissue development and defense regulation. Moreover, more and more evidence has shown that some members of the TIFY gene family perform their functions by modulating plant hormone signaling pathways. Poplar trees are found worldwide, and they comprise approximately 30 species. Benefit from the importance of poplar and its advanced platform, this tree is considered to be the model perennial plant. Here, we conducted a genome-wide identification of TIFY genes in poplar, and 24 TIFY genes were found. These 24 TIFY genes were assigned to different subfamilies according to the presence or absence of domains and motifs that they harbored. Careful analyses of their locations, structures, evolution and duplication patterns revealed an overview of this gene family in poplar. The expression profiles of these 24 TIFY genes were then analyzed in different tissues using publicly available expression data; their expression profiles following different JA/SA treatments and infection with leaf rust pathogen were also carefully examined by qRT-PCR assays. Based on their expression profiles, the functions of a number of TIFY genes could be predicted. By performing this study, we have provided valuable information for further functional characterisation of TIFY genes in poplar and candidate genes for the improvement of poplar disease resistance.

Genome-Wide Identification, Expression Profile of the TIFY Gene Family in Brassica oleracea var. capitata, and Their Divergent Response to Various Pathogen Infections and Phytohormone Treatments

“…WGD is one of the important mechanisms of species evolution, producing new genomic regions and making it more complex and diverse [63]. Similar conditions have been found in other species, such as 19 of the 24 poplar TIFY genes produced by WGD [64]. The large proportion of duplicated genes in the TIFY family revealed that WGD made a large contribution to the generation of the TIFY gene family.…”

Comprehensive Analysis of the TIFY Gene Family and Its Expression Profiles under Phytohormone Treatment and Abiotic Stresses in Roots of Populus trichocarpa

“…TIFY is a plant-specific transcription factor that has been identified in many model plants, such as Arabidopsis, Rice, and Maize [1,3,5]. Previous studies have given a comprehensive analysis of 24 PtrTIFY genes in leaves of P. trichocarpa under various phytohormone treatments and abiotic stresses [19,20]. In this study, we found a new PtrTIFY gene, PtrJAZ13, that belongs to the important JAZ subfamily.…”

“…In summary, different TIFY subfamily members have different functions during plant growth and development, as well as different responses to phytohormone treatment and abiotic stress.Populus trichocarpa serves as a model forest species and is widely used in forest tree genomics studies [18]. Previous works identified 24 PtrTIFY genes in the P. trichocarpa genome and analyzed their expression profiles under JA, MeJA, SA, salt, cold stresses, and pathogen infection in leaves [19,20]. In our study, besides these 24 genes, we found a new TIFY family member in the P. trichocarpa genome; because of the existence of the Jas and TIFY motif, we named it PtrJAZ13.…”

Genome-wide identification and expression analysis of jasmonate ZIM domain gene family in tuber mustard (Brassica juncea var. tumida)

“…According to the results of gene sequence identity and protein domain conservation analyses, 38 BjJAZ genes were identified in the genome of tuber mustard, and the number of BjJAZs is more than double that in Arabidopsis (13), rice (15) and Brachypodium distachyon (15) [27][28][29]. The reason might be that tuber mustard is an allotetraploid species formed by hybridization between the diploid ancestors of Brassica rapa and Brassica nigra with subsequent genome duplication [30]; genome duplication is also found in moso bamboo, the genome of which contains 18 PeJAZ genes [31].…”