Identification of Two Saccharomyces cerevisiae Cell Wall Mannan Chemotypes

Abstract

We have obtained evidence for two structurally and antigenically different Saccharomyces cerevisiae cell wall mannans. One, which occurs widely and is found in S. cerevisiae strain 238C, is already known to be a neutral mannan which yields mannose, mannobiose, mannotriose, and mannotetraose on acetolysis of the (1 a 6)-linked backbone. The other, which was found in S. cerevisiae brewer's strains, is a phosphomannan with a structure very similar to that of Kloeckera brevis mannan. S. cerevisiae (brewer's yeast strain) was agglutinated by antiserum prepared against Kloeckera brevis cells. The mannan, isolated from a proteolytic digest of the cell wall of the former, did not react with S. cerevisiae 238C antiserum, whereas it cross-reacted strongly with K. brevis antiserum. Controlled acetolysis cleaved the (1 6

Reassessment of Antigenic Determinant of Saccharomyces cerevisiae Serotype Ia

“…Rosenfeld and Ballou (20) reported that S. cerevisiae X2180 phosphomannan, with a mannose-to-phosphate ratio of 38, contained phosphorylated side chains. Furthermore, S. cerevisiae 1406 phosphomannan, with mannose-to-phosphate ratio of 19, possess a mannosylphosphorylmannotriose side chain, in which the mannosylphosphate group is immunodominant (5). These strains can be assumed to be different serotype from serotype la, although these strains remain to be serotyped.…”

“…On the other hand, Suzuki et al (21) analyzed the determinant group of S. cerevisiae X2180 mannan by the precipitin-inhibition assay using mannooligosaccharides as inhibitory haptens. Their results indicated that the mannotetraose, ( (5) established that the a-n-mannosylphosphate unit was also the major antigenic determinant in the side chains of some other strains of S. cerevisiae. Furthermore, Ballou et al (2) reported the generalized structures for the mannans of S. chevalieri, S. italicus, S. diastaticus, and S. carlsbergensis, all of which are now synonyms for S. cerevisiae (26), and indicated that in the S. cerevisiae system, the terminal a(1-3)-linked mannose unit was the principal immunochemical determinant on the cell surface.…”

“…With drastic changes of the classification of Saccharomyces species (26), as well as with three or more different immunochemical structures of mannans from S. cerevisiae and related species which were proposed by several investigators (1,5,8,21), considerable confusions have arisen. To clarify such confusions, as a first step we reexamined antigenic relationship between S. cerevisiae serotypes Ia and Ib and analyzed specific determinant of S. cerevisiae Ia mannan mainly by using 1H-nuclear magnetic resonance (NMR) spectral analyses (H-1 region) of acetolysis fragments of alkali-extracted mannan and by the agglutination-inhibition experiments with acetolysis fragments.…”

Some Considerations of the Flocculation Characteristics of Ale and Lager Yeast Strains*

“…Further aspects regarding co-floccula tion in ale yeast strains will be discussed later. 3. Strains flocculent on their own after growth in wort (i.e.…”

Sorption of volatile phenols by yeast cell walls

“…Various authors have identifi ed the phosphorylated side chains of the yeast mannoproteins, responsible for the negative charges of yeast surface, and have reported slight differences between S. cerevisiae strains. [38][39][40] On carrying out the same experiment with magnetic stirring, an increase in the sorption of both compounds was observed in comparison with the unstirred samples; the sorption was more important in the case of 4-ethylphenol than in the case of 4-ethylguaiacol. The concentration of 4-ethylphenol, after reaching the equilibrium in the experiment with stirring diminished compared to the control sample (15.1%), and in the case of 4-ethylguaiacol this reduction was somewhat lower (12.1%) (Figures 3a, 3b).…”