Identification of Wilms' Tumor 1-associating Protein Complex and Its Role in Alternative Splicing and the Cell Cycle

Abstract

Background: WTAP is a ubiquitously expressed nuclear protein that is required for mammalian early embryo development and cell cycle progression. Results: WTAP forms a complex with several splicing regulators. Conclusion: WTAP regulates both the cell cycle and alternative splicing by the formation of a protein complex. Significance: Characterization of this protein complex will help to elucidate the critically important function of WTAP in alternative splicing and cell proliferation.

BCLAF1 and its splicing regulator SRSF10 regulate the tumorigenic potential of colon cancer cells

“…RS domain is typical of splicing factors that are involved in pre-mRNA biogenesis and pre-mRNA splicing 8,10 . Consistent with this, findings from several groups indeed indicate a role of BCLAF1 in pre-mRNA splicing 4,35 . As phosphorylated RS domain of splicing factors is well known to mediate multiple protein-protein interactions 8 , it is possible that different protein interactions involving BCLAF1 might induce distinct functions at different cellular contexts.…”

PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells

“…RBM15 is required to maintain the homeostasis of long-term hematopoietic stem cells and the differentiation of MK cells (10,11). RBM15 protein stability is controlled by PRMT1 (5).…”

“…The lentiviral vector, pTripZ (Open Biosystems; Dharmacon), was engineered to express PRMT1 by replacing the shRNA cassette with the PRMT1 coding sequence to achieve robust PRMT1 expression in hematopoietic cells. A total of 15 µg lentivirus plasmids expressing the cDNA of PRMT1 V2, short hairpin (sh)RBM15#1 and shRBM15#2 (11,12) were transfected into 293T cells (American Type Culture Collection, Manassas, VA, USA) with helper plasmids. The 293T cells were cultured in Dulbecco's modified Eagle's medium with 10% FBS at 37˚C in an incubator with 5% CO 2 .…”

RNA N6-methyladenosine methylation in post-transcriptional gene expression regulation

“…1; Liu et al 2014;Ping et al 2014). WTAP was initially shown to act as a splicing factor that binds to Wilms' tumor 1 protein (Little et al 2000) and plays a regulatory role in cell cycle progression and early embryo development (Horiuchi et al 2006(Horiuchi et al , 2013. The first evidence of WTAP as a third component of the methyltransferase complex came from the coimmunoprecipitation result, which showed that WTAP readily binds to the METTL3-METTL14 heterodimer inside cells, although the interactions between WTAP and the two methyltransferases are weaker compared with that between METTL3 and METTL14 .…”