Identifikasi Deksametason dalam Jamu Pegal Linu Sediaan Serbuk yang Beredar di Pasar-pasar Kota Bandar Lampung secara Kromatografi Lapis Tipis

Abstract

A total of 26.6% of the population in Lampung Province consumed traditional medicine, one of which was Jamu Pegal Linu. However, a large public interest in herbal products was often misused by herbal manufacturers that it was possible to add BKO (Medicinal Chemicals), such as dexamethasone.The purpose of this study was to identify the presence or absence of dexamethasone in the preparation of Jamu Pegal Linu. The samples were taken from the store and depot herbs contained in the Markets of Bandar Lampung, acquired three different brands. The method used is Thin Layer Chromatography using dichloroethanediethyl ether-methanol-water (77:15: 8:1,2) as a mobile phase which were non-polar, and silica gel GF 254nm as a stationary phase. The result showed that the detection of three samples of Jamu Pegal Linu contained staining purple and the difference of Rf sample with reference standard on the first repetition is 0.46, 0.44, 0.47, and on the second repetition is 0.45, 0.45 and 0.48, which are ≥ 0.05. It can be concluded that the sample of Jamu Pegal Linu in Bandar Lampung 0% containing dexamethasone.

Analisis Sediaan Jamu Tradisional Yang Mengandung Bahan Kimia Obat Antihistamin Dan Kortikosteroid Dengan Metode Kromatografi Lapis Tipis

“…Pada pengujian deksametason, terdapat 7 penelitian yang menganalisis kandungan deksametason dalam jamu yaitu Permanasari, (2019), Lovianasari, et al, (2021), Khoirunnisa, (2018), Purnama, et al, (2018, Yuliastuti & Ramadhan, (2021), Prayoga, et al, (2016, Roni & Minarsih, (2021). Pada penelitian tersebut fase diam yang digunakan sama yaitu silika gel GF254 sedangkan dalam fase gerak menggunakan pelarut yang berbeda-beda.…”

Simple Analytical Method on the Determination of Dexamethasone in Herbal Medicine

“…e preparation steps are a modified method from Khoirunnisa's research with the addition of the centrifuge step after mixing the sample with the solvent. Each sample was weighed at 6 g and then dissolved in 30 mL of a mixture of chloroform: methanol (9 : 1, v/v) [13]. is solution was then shaken for 30 minutes and centrifuged for 5 minutes at 4000 rpm [4].…”

“…e filtrate was taken and evaporated over a water bath at a temperature of 70 °C, the remaining evaporation was dissolved in 5 ml of methanol [13]. is preparation performed well on blanks, negative control, and positive control.…”

Analisis Rhodamin B Dalam Lipstick Di Kecamatan Banjarmasin Utara

“…Metode penelitian yang dilakukan pada penelitian ini terbagi menjadi metode kualitatif untuk mengetahui keberadaan rhodamin B dan kuantitatif untuk menghitung kadar rhodamin B pada sampel. Adapun penelitian kualitatif dilakukan dengan Kromatografi Lapis Tipis (KLT) yang merupakan metode pemisahan campuran senyawa menjadi senyawa murninya dengan menggunakan dua fase, yaitu fase gerak dan fase diam (Khoirunnisa, 2019). Adapun fase gerak dan fase diam pada penelitian ini berturut-turut adalah etanol 70%: ammonia: etil asetat (5:2,5:12,5) dan silika gel 254 nm.…”