Identifying the geographical origin of protected sea cucumbers (Apostichopus japonicus) in China using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)

Abstract

Dalian sea cucumber, Yantai sea cucumber, and Weihai sea cucumber, which belong to , are protected as geographical indications in China based on their high nutritional values and medical propertys. The 26 samples, including Dalian sea cucumbers (9 samples) in Liaoning province, Yantai sea cucumbers (9 samples), and Weihai sea cucumbers (8 samples) in Shandong province, were individually collected from the designated geographical sea areas and the genetic relationships and DNA polymorphisms were evaluated by random amplified polymorphic DNA technology and gene segments sequencing. The RAPD dendrogram showed that the genetic diversity of the three types of sea cucumbers was rich. The neighbor-joining tree shows that the genetic relationship of the samples from the adjacent sea areas is closer. It demonstrates that the gene characteristics of sea cucumbers from different sea areas were obvious and the genetic diversity analysis by RAPD-PCR can be used as a rapid method for geographical discrimination.

Potential biological markers by DNA‐based tools for determination of Greek PDO geographical origin and authenticity: “Avgotaracho Mesolonghiou” and “Vostizza currant”

“…PCR‐RAPD band profiles of currants samples proved to be unique for each sample and can clearly be discriminated regarding their provenance. There are several studies that have already utilized PCR‐RAPD analysis for this purpose, such as geographical origin of sea cucumber, authentication of tissues of animal origin, authentication of plant Senna angustifolia or herb Lonicera japonica 40–43 …”

“…There are several studies that have already utilized PCR-RAPD analysis for this purpose, such as geographical origin of sea cucumber, authentication of tissues of animal origin, authentication of plant Senna angustifolia or herb Lonicera japonica. [40][41][42][43] Furthermore, analysis of PCR-RFLP results after digestion with selected restriction enzymes provided a rapid discrimination technique among samples. This method can be applied as a tool for improving mislabeling issues, traceability, and authentication.…”

Chemical substances and their activities in sea cucumber Apostichopus japonicus: A review

“…[ 5 ] In China, consumers generally believe that A. japonicus in Liaoning province has the best nutritional quality, and A. japonicus in Shandong province has the best culinary and sensory quality. [ 6,7 ]…”

“…[5] In China, consumers generally believe that A. japonicus in Liaoning province has the best nutritional quality, and A. japonicus in Shandong province has the best culinary and sensory quality. [6,7] After article retrieval, although reviews of sea cucumber polysaccharides, peptides, triterpene glycosides, or only a chemical component have been reported, there is only one comprehensive analysis review on literature philological research of A. japonicus species. [8] It is necessary to conduct a comprehensive evaluation from such a research hotspot with abundant high-level papers of its edible and medicinal value.…”

Preventive Effect of Collagen Peptides from Acaudina molpadioides on Acute Kidney Injury through Attenuation of Oxidative Stress and Inflammation

“…Sea cucumber is a precious aquatic product with high protein, low fat, low sugar, and low cholesterol [ 10 ]. It was recorded that sea cucumber has a nourishing effect on the liver and kidney [ 11 ].…”