IgE–Anti-IgE-Induced Prostaglandin D2Release from Cultured Human Mast Cells

Obata, Toru; Nagakura, Toshikazu; Kanbe, Masahiro; Masaki, Takuro; Maekawa, Kihei; Yamashita, Kowa

doi:10.1006/bbrc.1996.1287

Cited by 16 publications

(19 citation statements)

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“…It has been shown earlier that nordihydroguaiaretic acid completely blocks the generation of leukotrienes and partially inhibits prostaglandin production from RBL-2H3 mast cells after IgE-or calcium ionophore-mediated activation. Also, indomethacin was reported to inhibit prostanoid generation via inhibition of the cyclooxygenase pathway (Andersson et al, 1983;Chakravarty, 1984;Obata et al, 1996). Indeed, we found that the substance P-induced production of the arachidonic acid metabolites could be inhibited with nordihydroguaiaretic acid and indomethacin.…”

mentioning

confidence: 67%

Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Karimi

Kool

Nijkamp

et al. 2004

European Journal of Pharmacology

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Mast cells play a central role in immediate type hypersensitivity and inflammatory events. Activation of mast cells not only can result in the release of preformed granule-associated mediators generally followed by de novo synthesis of lipid-derived substances. In the present study, we show that mast cell can be activated to release lipid mediators in absence of granule exocytosis. Primary cultured murine mast cells were stimulated with substance P and produced leukotriene C 4 , and prostaglandin D 2 without the release of the granule-associated enzyme hhexosaminidase. Indomethacin and nordihydroguaiaretic acid caused complete inhibition of arachidonic metabolite generation. Leukotriene C 4 and prostaglandin D 2 production was blocked by genistein, a specific inhibitor of tyrosine kinases, and bisindolylmaleimide, a protein kinase C inhibitor, indicating a role for both phosphorylation pathways in the substance P-stimulated lipid mediator production. We suggest that the cytokine microenvironment of the mast cell determines whether mast cell stimulation leads to only lipid mediator release or full activation. Analysis of granule-associated mediators only might underestimate the role of mast cell activation under (patho)physiological conditions. D

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mentioning

confidence: 67%

Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Karimi

Kool

Nijkamp

et al. 2004

European Journal of Pharmacology

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“…For EPA pre-incubation experiments, EPA was added directly to the culture media (2-3 × The challenge incubation of cultured mast cells with IgEanti-IgE was similar to that reported previously [7]. Briefly, harvested mast cells were resuspended in Media I containing human myeloma IgE (1 mg/mL, Chemicon International Inc., Temecula, CA, USA), after which 1 mL of cell suspension (5 × 10 5 cells/mL) from each tube was incubated for 60 min at 37 ЊC.…”

Section: Epa Incubation and Ige-anti-ige Challenge Incubation With Inmentioning

confidence: 99%

“…Mast cells contain two types of cyclo-oxygenase (COX-1 and COX-2) under different physiological conditions [14]; cultured human mast cells contain both types of cyclo-oxygenase [7]. Mast cells contain two types of cyclo-oxygenase (COX-1 and COX-2) under different physiological conditions [14]; cultured human mast cells contain both types of cyclo-oxygenase [7].…”

Section: Introductionmentioning

confidence: 99%

Eicosapentaenoic acid inhibits prostaglandin D₂ generation by inhibiting cyclo‐oxygenase‐2 in cultured human mast cells

Obata

Nagakura

Masaki

et al. 1999

Clin Experimental Allergy

101

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Pre-incubation with EPA primarily affects the COX-2 pathway in cultured human mast cells and reduces PGD2 generation in response to IgE-anti-IgE challenge incubation. These findings suggest that COX-1 and COX-2 have different substrate flow systems in mast cells. They also suggest that endogenous EPA diet supplementation would reduce PGD2 production and could serve as an anti-inflammatory substrate in human mast cells.

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“…Mast cells derived from human umbilical cord blood monocytes were cultured for 12 weeks as previously described [1]. Cytocentrifuge smears were fixed for 30 min with Carnoy's solution (Ethanol : chloroform :glacial acetic acid, 6:3:1) or formal-saline (formalin : 0.85 % NaCl, 1:9) before the alcian blue (1% in 0.5 N HCl)/safranin (0.5 % in 0.125 N HCl) counter-staining procedure [3].…”

Section: Methodsmentioning

confidence: 99%

“…Most of our current knowledge on mast cell biology is therefore still derived largely from studies using rodent mast cells. Recently, however, homogeneous populations of human mast cells, containing tryptase positive basophilic granules after culturing in Steel Factor, IL-6 and PGE 2 for eight weeks, have been successfully cultured from human umbilical cord blood [1,2]. In the current study, we characterised these cultured mast cells by studying their staining properties and their responses to a range of mast cell activators and inhibitors.…”

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confidence: 99%

Some characteristics of mast cells cultured from human umbilical cord blood

Lau¹,

Obata

Nagakura

et al. 2000

Inflamm. res.

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IntroductionAlthough significant advances have been made in the biology of human mast cells by using enzymatically isolated tissue mast cells there are still difficulties in obtaining large numbers of these cells with sufficient purity. Most of our current knowledge on mast cell biology is therefore still derived largely from studies using rodent mast cells. Recently, however, homogeneous populations of human mast cells, containing tryptase positive basophilic granules after culturing in Steel Factor, IL-6 and PGE 2 for eight weeks, have been successfully cultured from human umbilical cord blood [1,2]. In the current study, we characterised these cultured mast cells by studying their staining properties and their responses to a range of mast cell activators and inhibitors. Materials and methodsMast cells derived from human umbilical cord blood monocytes were cultured for 12 weeks as previously described [1]. Cytocentrifuge smears were fixed for 30 min with Carnoy's solution (Ethanol : chloroform :glacial acetic acid, 6:3:1) or formal-saline (formalin : 0.85 % NaCl, 1:9) before the alcian blue (1% in 0.5 N HCl)/safranin (0.5 % in 0.125 N HCl) counter-staining procedure [3]. Cells were sensitized by incubating in 1 mg/ml human myeloma IgE for two hours at 37°C. Cells were then washed before being activated by either anti-IgE or other secretagogues with or without inhibitors. Reactions were stopped 10 min later by the addition of ice cold buffer and cells sedimented by centrifugation. Histamine content in both supernatants and cell pellets was determined spectrofluorometrically [3]. Results and discussionCultured human mast cells were typically more than 90 % viable and contained 1.68 ± 0.3 pg/cell of histamine (n = 10). Although all cells in cytocentrifuge smears with or without fixation stained with alcian blue and failed to be counter stained by safranin, smears fixed with formal saline were less well stained. Previous studies have demonstrated that human tissue mast cells, although different in their fixation properties (skin is formaldehyde insensitive whereas lung is formaldehyde sensitive), all stained with alcian blue after the alcian blue/safranin counterstaining process. The cultured human mast cells hence shared the common staining property of human tissue mast cells and resembled human lung mast cells in their formaldehyde sensitivity [4]. Fig. 1. Effects of secretagogues on cultured human mast cells. Results are expressed as mean percentage releases of total histamine contents ± SEM for 6 experiments corrected for spontaneous secretions (5.3 ± 1.1 %).

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IgE–Anti-IgE-Induced Prostaglandin D2Release from Cultured Human Mast Cells

Cited by 16 publications

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Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Eicosapentaenoic acid inhibits prostaglandin D₂ generation by inhibiting cyclo‐oxygenase‐2 in cultured human mast cells

Some characteristics of mast cells cultured from human umbilical cord blood

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IgE–Anti-IgE-Induced Prostaglandin D2Release from Cultured Human Mast Cells

Cited by 16 publications

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Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Substance P can stimulate prostaglandin D2 and leukotriene C4 generation without granule exocytosis in murine mast cells

Eicosapentaenoic acid inhibits prostaglandin D2 generation by inhibiting cyclo‐oxygenase‐2 in cultured human mast cells

Some characteristics of mast cells cultured from human umbilical cord blood

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Eicosapentaenoic acid inhibits prostaglandin D₂ generation by inhibiting cyclo‐oxygenase‐2 in cultured human mast cells