Ileal digestibility of amino acids, phosphorus, phytate and energy in pigs fed sorghum-based diets supplemented with phytase and Pancreatin®

Abstract

The effects of phytase supplementation on the apparent ileal digestibility (AID) of amino acids (AA) have been inconsistent. Two experiments evaluated the effect of providing a mixture of pancreatic enzymes (Pancreatin(®) ) to growing pigs fed sorghum-soybean meal diets supplemented with phytase on the AID of AA, energy, and phosphorus (P), as well as the ileal digestibility (ID) of phytate; there were four periods per experiment. In Experiment 1, eight pigs (BW 22.1±1.3 kg) were fitted with a T-cannula at the distal ileum. Each period consisted of 9 days; 7 days for diet adaptation, and 2 days for digesta collection. Treatments (T) were: (i) basal sorghum-soybean meal diet, (ii) basal diet plus Pancreatin®, (iii) basal diet plus phytase and (iv) basal diet plus phytase and Pancreatin®. Phytase increased the digestibilities of phytate and P (p<0.001), but did not affect the AID of AA and energy (p>0.10). Except for methionine (p=0.07), Pancreatin® did not affect the AID of AA. Phytase and Pancreatin® did not interact (p>0.10). Experiment 2 was similar to Experiment 1, but Pancreatin® was infused into duodenum. Pancreatin® infusion did not affect the AID of AA (p>0.10); and tended to reduce (p=0.09) the AID of lysine. Phytase × Pancreatin® interactions were not observed (p>0.10). In conclusion, phytase and Pancreatin® did not improve the AID of AA in growing pigs fed sorghum-soybean meal diets indicating that phytates did not affect AA digestibility.

Effect of Phytase Derived from the E. coli AppA Gene on Weaned Piglet Performance, Apparent Total Tract Digestibility and Bone Mineralization

“…This is in agreement with a study on poultry, in which the addition of a phytase product (RONOZYME HiPhos) at 5000 U/g to the diets (at 0.04%) containing different protein sources (soybean meal, yellow lupin meal, and blue lupin meal) did not affect the ATTD of the protein [16]. Similarly, Cervantes et al [26] conducted a study on swine fed with sorghum-soybean meal diets supplemented with phytase and observed no improvement in the ATTD of amino acids. Zeng et al [27] found that the ATTD of crude protein was significantly increased in piglets (20-25 kg) fed a diet with 0.14% non-phytate-P and 0.38% total P only at a concentration of 20,000 FTU/kg and not at 500 or 1000 FTU/kg.…”

Nutrient digestibility in diets containing low-phytate barley, low-phytate field pea and normal-phytate field pea, and the effects of microbial phytase on energy and nutrient digestibility in the low and normal-phytate field pea fed to pigs

“…However, the effects of phytase supplementation on AA and CP digestibility have not been consistent. For example, studies by Liao et al (2005), Woyengo et al (2008) and Cervantes et al (2011) found no significant effects due to phytase supplementation. In the present study, there was an interaction of pea cultivar and phytase in some AA, because phytase supplementation increased AA digestibility in NPP and the specific AA were: Ile, met, Phe, Val, Ala, Asp, Gly, Pro and Ser.…”

“…To minimize the effects of phytate, dietary supplementation with phytase has been successfully used to improve P digestibility in pigs (Harper et al, 1997;Woyengo et al, 2009). However, the effect of phytase supplementation on AA and energy digestibility is variable (Sands et al, 2009;Kiarie et al, 2010;Cervantes et al, 2011;Selle et al, 2012;Woyengo and Nyachoti, 2013).…”

Phytate in pig and poultry nutrition

“…However, Champagne et al (1990) suggested that ternary protein-phytate complexes may not have the capacity to bind sufficient protein to be important in respect of AA digestibility, due to reason that protein would be mainly present as lower molecular weight peptides. This might explain the lack of effect of phytase on protein digestibility in several studies (N€ asi et al, 1995;Traylor et al, 2001;Cervantes et al, 2011).…”

“…Trypsin is the most critical pancreatic protease, because it autocatalysis its activation and subsequently activates the other proteases. Cervantes et al (2011) suggested that if the activity of pancreatic proteases is reduced by phytates, the application of exogenous pancreatic enzymes including trypsin would overcome this effect. However, they did not find any effect on the AID of protein or AA.…”