Imaging and Analyzing the Elasticity of Vascular Smooth Muscle Cells by Atomic Force Acoustic Microscope

Zhang, Bo; Cheng, Qian; Chen, Ming; Yao, Wengang; Mei, Qian; Hu, Bing

doi:10.1016/j.ultrasmedbio.2012.04.002

Cited by 12 publications

(6 citation statements)

References 36 publications

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“…CR modes have been generally used on relatively stiff materials, while still finding applications in biomaterials and polymers . AFAM has been used to map elasticity in antler, teeth, nacre, and recently smooth muscle cells, while UAFM has been used to image the elasticity of bacterial fragments . Complementary electromechanical information can be obtained about biological samples using piezoresponse force microscopy in combination with CR modes .…”

Section: Recent Developmentsmentioning

confidence: 99%

Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Kilpatrick

Revenko

Rodriguez

2015

Adv Healthcare Materials

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The behavior and mechanical properties of cells are strongly dependent on the biochemical and biomechanical properties of their microenvironment. Thus, understanding the mechanical properties of cells, extracellular matrices, and biomaterials is key to understanding cell function and to develop new materials with tailored mechanical properties for tissue engineering and regenerative medicine applications. Atomic force microscopy (AFM) has emerged as an indispensable technique for measuring the mechanical properties of biomaterials and cells with high spatial resolution and force sensitivity within physiologically relevant environments and timescales in the kPa to GPa elastic modulus range. The growing interest in this field of bionanomechanics has been accompanied by an expanding array of models to describe the complexity of indentation of hierarchical biological samples. Furthermore, the integration of AFM with optical microscopy techniques has further opened the door to a wide range of mechanotransduction studies. In recent years, new multidimensional and multiharmonic AFM approaches for mapping mechanical properties have been developed, which allow the rapid determination of, for example, cell elasticity. This Progress Report provides an introduction and practical guide to making AFM-based nanomechanical measurements of cells and surfaces for tissue engineering applications.

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Section: Recent Developmentsmentioning

confidence: 99%

Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Kilpatrick

Revenko

Rodriguez

2015

Adv Healthcare Materials

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“…As expected, the Young's modulus of glass did not alter significantly over the length of the sample (range of 550 MPa to 590 MPa). Here it has to be noted that the “real” value for glass is most likely higher, since these values represent a minimum value and a glass stiffness in the GPa range is reported in the literature . Glass was used as a non‐compressible material for calibration and hence does not provide an accurate value but only a minimum; however, it clearly shows that the relative change across the gradient is only marginal as compared to PDMS.…”

Section: Figurementioning

confidence: 96%

Double Linear Gradient Biointerfaces for Determining Two‐Parameter Dependent Stem Cell Behavior

et al. 2016

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Two-parameter contributionso fs tiffness and wettability display alteredb ehavior of hBM-MSCs as compared to solely investigating the wettability of the biointerface with ah omogenous stiffness. The importance of double parameter influence is identified by using newly developed double linear wettability-stiffness gradients. The interfaces show that substrate stiffness can influence cell adhesion and spreading both positivelya nd negatively in combination with wettability.T his effect is validated by decoupling the stiffness gradientu sing aw ettability gradient composed of amonolayer on glass ensuring ahomogenous stiffness.

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“…[293] CR modes have been generally used on relatively stiff materials, [285,286] while still finding applications in biomaterials and polymers. [294] AFAM has been used to map elasticity in antler, [295] teeth, [296] nacre, [297] and recently smooth muscle cells, [298] while UAFM has been used to image the elasticity of bacterial fragments. [299] Complementary electromechanical information can be obtained about biological samples using piezoresponse force microscopy in combination with CR modes.…”

Section: Recent Developmentsmentioning

confidence: 99%