Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Kilpatrick, Jason I.; Revenko, Irène; Rodriguez, Brian J.

doi:10.1002/adhm.201801142

Cited by 6 publications

(9 citation statements)

References 302 publications

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“…This system combines key features comparable to other leading techniques, with a regular array of probes that can map the mechanical response, as is possible with atomic force microscopy (22,(63)(64)(65), and measure cellular fluctuations and local rheology simultaneously with high temporal resolution and ligand specificity, as with magnetic twisting cytometry (4,44,45). In this study, the use of AMPADs has allowed us to make a detailed examination of the mechanics of the cell cortex and stress fibers.…”

Section: Discussionmentioning

confidence: 99%

Dissecting fat-tailed fluctuations in the cytoskeleton with active micropost arrays

Shi

Porter

Crocker

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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The ability of animal cells to crawl, change their shape, and respond to applied force is due to their cytoskeleton: A dynamic, cross-linked network of actin protein filaments and myosin motors. How these building blocks assemble to give rise to cells’ mechanics and behavior remains poorly understood. Using active micropost array detectors containing magnetic actuators, we have characterized the mechanics and fluctuations of cells’ actomyosin cortex and stress fiber network in detail. Here, we find that both structures display remarkably consistent power law viscoelastic behavior along with highly intermittent fluctuations with fat-tailed distributions of amplitudes. Notably, this motion in the cortex is dominated by occasional large, step-like displacement events, with a spatial extent of several micrometers. Overall, our findings for the cortex appear contrary to the predictions of a recent active gel model, while suggesting that different actomyosin contractile units act in a highly collective and cooperative manner. We hypothesize that cells’ actomyosin components robustly self-organize into marginally stable, plastic networks that give cells’ their unique biomechanical properties.

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Section: Discussionmentioning

confidence: 99%

Dissecting fat-tailed fluctuations in the cytoskeleton with active micropost arrays

Shi

Porter

Crocker

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…To characterize the mechanoelasticity of model biomembranes before and after incubation with a RTIL/water solution below the CMC, we utilized atomic force microscopy (AFM), a scanning probe microscopy technique that has been successfully used to study the mechanoelasticity of model biomembranes and living cells in different environments (Garcia-Manyes and Sanz 2010; Monocles et al 2010;Roa et al 2011;Ferenc et al 2012;Kilpatrick et al 2015;Stetter et al 2016). AFM is essentially composed of a laser, a photodiode, and a cantilever ending with a sharp tip that acts as the probe and can be used to image the surface of the system and to perform force measurements ( Fig.…”

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confidence: 99%

Controlling the mechanoelasticity of model biomembranes with room-temperature ionic liquids

et al. 2018

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Room-temperature ionic liquids (RTILs) are a vast class of organic non-aqueous electrolytes whose interaction with biomolecules is receiving great attention for potential applications in bio-nano-technology. Recently, it has been shown that RTILs dispersed at low concentrations at the water-biomembrane interface diffuse into the lipid region of the biomembrane, without disrupting the integrity of the bilayer structure. In this letter, we present the first exploratory study on the effect of absorbed RTILs on the mechanoelasticity of a model biomembrane. Using atomic force microscopy, we found that both the rupture force and the elastic modulus increase upon the insertion of RTILs into the biomembrane. This preliminary result points to the potential use of RTILs to control the mechanoelasticity of cell membranes, opening new avenues for applications in bio-medicine and, more generally, bio-nano-technology. The variety of RTILs offers a vast playground for future studies and potential applications.

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“…Schematic diagram of the adhesion in AFM nanoindentation, in which the red line is the approach and the blue line is the retract curve. Reproduced with permission from [86].…”

Section: Adhesion and Point Of Contact (Poc)mentioning

confidence: 99%

“…Aside from the theories based on adhesive contact, some methods for non-adhesive contact have been employed for POC determination, and summed up in the literature [86,87]. These include, but are not limited to, visual inspection [112], model fitting [105], extrapolation [112,113], and Bayesian analysis [114].…”

Section: Adhesion and Point Of Contact (Poc)mentioning

confidence: 99%

“…Overall, compared with the Oliver-Pharr model based on elastic-plastic deformation (or the "correction" based on viscous-elastic-plastic deformation), the Hertz and Sneddon models, based on elastic contact, may dominate the literature for soft biomaterials due to their simplicity and widespread application [86]. The comparisons between the Oliver-Pharr method and Hertz contact model are summarized in Table 1, in which some aspects, such as tip selection and control mode, will be further discussed in the next section.…”

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confidence: 99%

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Small Scale Deformation using Advanced Nanoindentation Techniques

Tsui¹,

Volinsky²

2019

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has over twenty-five years of experience in the integrated circuit (IC) fabrication industry and in the academic environment. At Texas Instruments Inc. (Dallas, Texas), Prof. Tsui developed Plasma Enhanced Chemical Vapor Deposited (PECVD) porous low-k and ultra-low-k thin films to be used for interlayer dielectric and dielectric barriers for 90 nm, 65 nm, and 45 nm technology node. He also developed plasma-based processes to enhance electrical and chip reliability performance. In addition to the unit process module development, Professor Tsui has also demonstrated his expertise in wafer-level integration, yield ramp, TDDB reliability, and in-fab and in-field mechanical reliability. In 2007, Professor Tsui began his role at the University of Waterloo (Ontario, Canada) where he has conducted research in the areas of porous ultra-low-dielectric constant materials, nano-mechanics, electron beam lithography, and nanoindentation.

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Nanomechanics of Cells and Biomaterials Studied by Atomic Force Microscopy

Cited by 6 publications

References 302 publications

Dissecting fat-tailed fluctuations in the cytoskeleton with active micropost arrays

Dissecting fat-tailed fluctuations in the cytoskeleton with active micropost arrays

Controlling the mechanoelasticity of model biomembranes with room-temperature ionic liquids

Small Scale Deformation using Advanced Nanoindentation Techniques

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