This work presents the first reported imbibition mechanism
of femtoliter
(fL)-scale droplets produced by microchannel cantilever spotting (μCS)
of DNA molecular inks into porous substrates (hydrophilic nylon).
Differently from macroscopic or picoliter droplets, the downscaling
to the fL-size leads to an imbibition process controlled by the subtle
interplay of evaporation, spreading, viscosity, and capillarity, with
gravitational forces being quasi-negligible. In particular, the minimization
of droplet evaporation, surface tension, and viscosity allows for
a reproducible droplet imbibition process. The dwell time on the nylon
surface permits further tuning of the droplet lateral size, in accord
with liquid ink diffusion mechanisms. The functionality of the printed
DNA molecules is demonstrated at different imbibed oligonucleotide
concentrations by hybridization with a fluorolabeled complementary
sequence, resulting in a homogeneous coverage of DNA within the imbibed
droplet. This study represents a first step toward the μCS-enabled
fabrication of DNA-based biosensors and microarrays into porous substrates.