Immersion digital in-line holographic microscopy

Garcı́a-Sucerquia, Jorge; Xu, Wenbo; Jericho, M. H.; Kreuzer, H. J.

doi:10.1364/ol.31.001211

Cited by 111 publications

(68 citation statements)

References 7 publications

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“…This technique now has applications in a large number of domains from investigation of particles in flows [2][3][4][5][6][7], to visualization of cells in biology or medicine, to phase contrast metrology, or to detection of nanoparticles without being exhaustive [8][9][10][11][12][13][14]. Research activities in this field have many orientations: some articles treat the elaboration of models to compute and predict holograms.…”

Section: Introductionmentioning

confidence: 99%

Numerical Models for Exact Description of in-situ Digital In-Line Holography Experiments with Irregularly-Shaped Arbitrarily-Located Particles

et al. 2015

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We present the development of a numerical simulator for digital in-line holography applications. In-line holograms of arbitrarily shaped and arbitrarily located objects are calculated using generalized Huygens-Fresnel integrals. The objects are 2D opaque or phase objects. The optical set-up is described by its optical transfer matrix. A wide variety of optical systems, involving windows, spherical or cylindrical lenses, can thus be taken into account. It makes the simulator applicable for design and description of in situ experiments. We discuss future applications of this simulator for detection of nanoparticles in droplets, or calibration of airborne instruments that detect and measure ice crystals in the atmosphere.

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Section: Introductionmentioning

confidence: 99%

Numerical Models for Exact Description of in-situ Digital In-Line Holography Experiments with Irregularly-Shaped Arbitrarily-Located Particles

et al. 2015

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“…One representative work is done by H.J. Kreuzer's group [1][2][3][4][5]. Their systems utilize Gabor in-line set-up to trace 4D trajactories of different biological samples with micrometer resolution.…”

Section: Introductionmentioning

confidence: 99%

Lens-free and portable quantitative phase microscope using a dual-pinhole aperture

Liu

Wang

et al. 2015

MATEC Web of Conferences

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Abstract. This paper presents a lens-free and portable quantitative phase microscope. This microscope employs a concise off-axis set-up where a dual-pinhole aperture is utilized to generate the reference wave and the object wave. As no lenses or beamsplitters are used in this microscope, the total size of this microscope is only slightly larger than a smart phone, and the cost of this microscope except for the digital camera is about 3000 RMB. Even with such small size and low cost, this microscope possesses a lateral resolution of ∼ 1.7μm and an axial accuracy of tens of nanometers.

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“…For n > 1; the IOPL represents an optically foreshortened distance [136], [161], [113]. This is especially important for oceanic holography, where the waves pass through water with an index of refraction n = 1:33 1:35 [291], [256] and at least one optical window with n ranging from 1:4 1:8 [155].…”

Section: Introduction To Digital Holographymentioning

confidence: 99%

Computational imaging and automated identification for aqueous environments

Loomis¹

2011

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Sampling the vast volumes of the ocean requires tools capable of observing from a distance while retaining detail necessary for biology and ecology, ideal for optical methods. Algorithms that work with existing SeaBED AUV imagery are developed, including habitat classi…cation with bag-of-words models and multi-stage boosting for rock…sh detection. Methods for extracting images of …sh from videos of longline operations are demonstrated.A prototype digital holographic imaging device is designed and tested for quantitative in situ microscale imaging. Theory to support the device is developed, including particle noise and the e¤ects of motion. A Wigner-domain model provides optimal settings and optical limits for spherical and planar holographic references.Algorithms to extract the information from real-world digital holograms are created. Focus metrics are discussed, including a novel focus detector using local Zernike moments. Two methods for estimating lateral positions of objects in holograms without reconstruction are presented by extending a summation kernel to spherical references and using a local frequency signature from a Riesz transform. A new metric for quickly estimating object depths without reconstruction is proposed and tested. An example application, quantifying oil droplet size distributions in an underwater plume, demonstrates the e¢ cacy of the prototype and algorithms. Course work at MIT and WHOI was helpful throughout this thesis. In particular, course materials and projects from Dr. Antonio Torralba (computer vision, detection, and boosting), Dr. Frédo Durand (computational imaging, segmentation, and probability), Dr. Rosalind Picard (machine learning, pattern recognition, and probability), Dr. Alan 4 Edelman (parallel computing and algorithms), and Dr. Barbastathis (optical systems) are re ‡ected directly, in several cases extended after the course to become complete sections of this thesis.The MIT Museum has provided unique outreach opportunities for the ideas presented in this thesis. Seth Riskin originally involved our lab in holography activities at the MIT Museum, then Kurt Hasselbalch later proposed and guided the development of an interactive display on plankton holography. The computational approaches I learned for the museum display led to further scienti…c development and spurred the use of GPUs for processing; Section 3.3.5 is a direct consequence, as is the majority of the data processing performed throughout this thesis.In reference to data processing, Matlab/MEX and NVIDIA's CUDA have been daily cornerstones for which I am continually thankful. Finally, mad props go to Dr. José "Pepe" Domínguez-Caballero, the most in ‡uential 5 person in my scienti…c development while at MIT. Pepe taught me digital holography and worked one-on-one with me throughout the beginning of my grad student career. He continued to be involved as a fellow holographer, a mentor, and a friend, discussing ideas, encouraging active experimentation, maintaining a curiosity about optics, and o¤ering eno...

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Immersion digital in-line holographic microscopy

Cited by 111 publications

References 7 publications

Numerical Models for Exact Description of in-situ Digital In-Line Holography Experiments with Irregularly-Shaped Arbitrarily-Located Particles

Numerical Models for Exact Description of in-situ Digital In-Line Holography Experiments with Irregularly-Shaped Arbitrarily-Located Particles

Lens-free and portable quantitative phase microscope using a dual-pinhole aperture

Computational imaging and automated identification for aqueous environments

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