Immobilization of collagen peptide on dialdehyde bacterial cellulose nanofibers via covalent bonds for tissue engineering and regeneration

Abstract

Bacterial cellulose (BC) is an alternative nanostructured biomaterial to be utilized for a wide range of biomedical applications. Because of its low bioactivity, which restricted its practical application, collagen and collagen hydrolysate were usually composited into BC. It is necessary to develop a new method to generate covalent bonds between collagen and cellulose to improve the immobilization of collagen on BC. This study describes a facile dialdehyde BC/collagen peptide nanocomposite. BC was oxidized into dialdehyde bacterial cellulose (DBC) by regioselective oxidation, and then composited with collagen peptide (Col-p) via covalent bonds to form Schiff’s base type compounds, which was demonstrated by the results of microstructures, contact angle, Col-p content, and peptide-binding ratio. The peptide-binding ratio was further affected by the degree of oxidation, pH value, and zeta potential. In vitro desorption measurement of Col-p suggested a controlled release mechanism of the nanocomposite. Cell tests indicated that the prepared DBC/Col-p composite was bioactive and suitable for cell adhesion and attachment. This work demonstrates that the DBC/Col-p composite is a promising material for tissue engineering and regeneration.

Comparative study on the physical entrapment of soy and mushroom proteins on the durability of bacterial cellulose bio‐leather

“…3, the original BC had the lowest WCA. This is due to the free hydroxyl groups in BC, which exhibit hydrophilic properties (Wen et al 2015). BC-glycerol also had a low WCA, which was related to the hydrophilic behavior of glycerol (Epure et al 2011).…”

Advanced Functional Materials Based on Nanocellulose for Pharmaceutical/Medical Applications

“…Collagen (COL) is one of the major components of the extracellular matrix, recognized for its excellent biocompatibility and biodegradability, but also for its low bioactivity, which restricts its practical application [ 144 ]. To overcome this limitation, Wen and coworkers [ 142 ] used dialdehyde bacterial cellulose (DBC) to composite collagen peptide (Col-p) via covalent bonds between the aldehyde group of DBC and amino functional groups of Col-p. The nanocomposites DBC/Col-p were prepared by immersing DBC with various degrees of oxidation into Col-p solution and explored for the release of collagen peptides compared to BC/Col-p.…”

“…The cells number increased obviously starting on the 4th day and continued to grow on the 7th day, in well-spread ways, when the confluence also occurred. In conclusion, the DBC/Col-p nanocomposites showed suitable fibroblast cell adhesion and proliferation and proved to be a promising material for tissue engineering and regeneration [ 142 ].…”

Nanocellulose nanocomposite hydrogels: technological and environmental issues

“…The hydrogels exhibited a lower degree of swelling, stability and mechanical resistance when compared to control gels containing only BNC. The formation of a Schiff base between BNC with aldehyde groups and collagen was explored by Wen et al 108 for the release of collagen peptides. The hydrogel showed good cell adhesion and proliferation of Westar rat fibroblast cells cultured in vitro.…”