Immobilization of Detonation Nanodiamonds on Macroscopic Surfaces

Abstract

Detonation nanodiamonds (NDs) are a novel class of carbon-based nanomaterials, and have received a great deal of attention in biomedical applications, due to their high biocompatibility, facile surface functionalization, and commercialized synthetic fabrication. We were able to transfer the NDs from large-size agglomerate suspensions to homogenous coatings. ND suspensions have been used in various techniques to coat on commercially available substrates of pure Ti and Si. Scanning electron microscopy (SEM) imaging and nanoindentation show that the densest and strongest coating of NDs was generated when using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide (EDC/NHS)-mediated coupling to macroscopic silanized surfaces. In the next step, the feasibility of DNA-mediated coupling of NDs on macroscopic surfaces is discussed using fluorescent microscopy and additional particle size distribution, as well as zeta potential measurements. This work compares different ND coating strategies and describes the straightforward technique of grafting single-stranded DNA onto carboxylated NDs via thioester bridges.

Modification of titanium implants using biofunctional nanodiamonds for enhanced antimicrobial properties

“…ND with concentration 0.5 mg/ml had a hydrodynamic size of 44 ± 1 nm and a zeta potential -29 ± 1 mV. The results are consistent with the values reported in literature [22,27]. After conjugation with PDDA, the hydrodynamic diameter of the 1 mg/ml sample increased to 395 ± 9 nm and zeta potential shifted to positive value of 54 ± 1 mV.…”

“…Bead-milled ND were diluted using ddH 2 O to final concentrations of 1 mg ml −1 and 0.5 mg ml −1 . The suspensions were stabilized by pH adjustment using NaOH [22]. After pH adjustment, the solutions were additionally exposed to 30 min of ultrasound treatment using Sonoplus HD 2200 ultrasonic homogenizer from Bandelin electronic GmbH and Co. KG, Berlin, Germany at 70% of the device power.…”

Pickering emulsions stabilised with oligoglycine-functionalised nanodiamond as a model system for ocular drug delivery applications

“…The synthesis partly followed the method reported in the literature 72 to modify ND to graft it to hydroxylated surfaces through a silane coupling. Firstly, 2.5 mL of ND dispersions were added into a 20 mL vial and were diluted to 2.5 mg mL À1 by adding 7.5 mL DI water.…”

Several Aspects of Application of Nanodiamonds as Reinforcements for Metal Matrix Composites

“…Carbon nanoparticles, including nanodiamonds (NDs), evoke considerable interest among researchers because of their unique properties [1][2][3][4][5][6][7][8][9][10][11][12]. The size of primary ND particles is generally 4-6 nm.…”