Immobilization of enzymes by multilayer microcapsules

Pommersheim, R.; Schrezenmeir, Jürgen; Vogt, Walter

doi:10.1002/macp.1994.021950508

Cited by 71 publications

(42 citation statements)

References 4 publications

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“…If no charges are contained inherently, ionic groups can be easily introduced by chemical modifications 360) . Multilayers containing alginic acid [361][362][363][364] , chondroitin sulfate 364,365) , heparin 26,253,351,366) , chitosan 42,215,365,[367][368][369][370] , chitosan sulfate 42) , cellulose sulfate 154,362,371,372) , dextran sulfate 26,352,355,366,370) , and carboxymethylcellulose 371,372) have indeed been occasionally assembled by ESA. Mostly, the use of polysaccharides was aimed at improving surface biocompatibility of the substrate materials, a property needed, e. g. for cell culture and human implants.…”

Section: Polymers Suited For Esamentioning

confidence: 98%

“…But even larger molecules can diffuse reasonably well into the layers 39,142,148,214,248,258,336,346,347,358,390,394) so that a rich chemistry is possible per se. Still, it must be kept in mind that the mobility of small molecules decreases not only with their size 250,251,258,347,361,372,452) , but also notably in deeper lying layers 41,86,142,214,250,251,270,333) (typically deeper than 5 -8 layers from the top), so that the homogeneity of chemical modifications in the layer normal may be questionable (cf. Fig.…”

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

See 1 more Smart Citation

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,174

1,184

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A bit of historyThe key role of surfaces for many material properties as well as for many biological processes has been recognized now. Therefore, new strategies aim at the tailoring of the material's surface only -or of a thin surface layer, respectively -, while preserving the bulk properties of the underlying support. Particular emphasis has been given to the surface modification by polymers, in an attempt to extend the known versatility of polymer bulk materials to ultrathin films and coatings, and to prepare bulk-surface composite materials. The self-organization of polymers has been increasingly explored for the preparation of well-defined surfaces and interfaces in recent years, extending the use of the established methods of low molar mass compounds (for comparative reviews, see ref. 1-3)). With such techniques, polymer films are formed spontaneously on substrates, due to balanced interactions between substrate, polymer (or its precursor) and medium. Typically, very thin, often monomolecular layers are produced. Repetitive deposition steps provide a precise control over the total thickness of the coatings, in the range from a few angstroms up to the micrometer range. Moreover, the step-by-step procedures allow for a fine structuring in the third dimension. In addition to the preparation of uniform and homogeneous coatings, gratings, gradients or steps of defined height in molecular dimensions can be easily constructed.The most recent of the self-organization techniques is the alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA) [3][4][5][6][7][8][9][10] . Although some early, singular studies of self-assembly by alternating adsorption of oppositely charged polyions are reported [11][12][13] , a practical method for ESA was developed Feature Article: The article presents the state-of-the-art of alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA), for the preparation of thin polymer coatings. In comparison to other, more established self-organization techniques, this recent method is distinguished by its simplicity, versatility, and speed. In particular, the tendency for self-healing is unique. Emphasis is given to the role of the molecular structure of the polyelectrolytes, and to the nature of the support. Also, various parameters for the preparation of multilayer films are highlighted, which are very important due to the kinetic control of the build-up process. The structure of the resulting coatings, their quality and stability, chemical reactions in the films, and potential applications are discussed.Macromol. Rapid Commun. 21, No. 7

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Section: Polymers Suited For Esamentioning

confidence: 98%

Section: Chemical Reactions In Films Made By Esamentioning

confidence: 99%

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,174

1,184

View full text Add to dashboard Cite

show abstract

“…Thin film applications range from coatings, compatibilization and surface protection [59], enzyme immobilization [60], biosensors [61], gas separation membranes [62], high charge density batteries [63], modified electrodes [64], etc. Polyelectrolyte multilayer microcapsules, on the other hand, may be used in areas such as medicine, delivery systems [65], catalysis, micro-containers and reactors [66]. The unique molecular structures of organometallic PFS polyelectrolytes also provide some special application potentials, which will be discussed in this section.…”

Section: Application Potentials Of Poly(ferrocenylsilane) Multilayer mentioning

confidence: 99%

Electrostatic Assembly with Poly(ferrocenylsilanes)

Hempenius

Vancso

2007

J Inorg Organomet Polym

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New frontiers in polymer science involve the incorporation of functional species into material systems. The electrostatic layer-by-layer (LBL) self-assembly technique constitutes a versatile tool for nano-and microscale fabrication of devices and novel material structures. Although a rapidly growing attention has been paid to this area, most of the studies conducted were based on organic polymeric electrolytes. Due to synthetic developments, new polymeric structures with inorganic elements and transition metals incorporated in the main chain have become accessible. With the development of new synthesis routes, organometallic poly(ferrocenylsilane) polycations and polyanions emerged. Their charged nature and water-solubility made them excellent candidates for the extension of electrostatic multilayer assembly to organometallic polymeric materials. The present review gives a concise summary on the LBL fabrication of organometallic thin films and microcapsules based on water-soluble poly(ferrocenylsilane) polyions. The unique functions of these structures come from the molecular structure of poly(ferrocenylsilanes), in which silicon atoms and redoxactive ferrocene units are present. In this context, the diverse application potentials of these organometallic multilayer structures are also discussed.

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“…It has been reported that the naturally occurring biopolymer, alginate, has a peculiar property to form a matrix gel, which could be exploited for encapsulation/delivery of a biomacromolecules (9,10). In the proposed systems, we have exploited the gel formation property of alginate and further to enhance the surface area for electrostatic interaction within the pores of core particles (CP) to improve the payload efficiency and to control the size of microreservoirs.…”

Section: Introductionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

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Abstract. The aim of the study was to develop ultrathin polyelectrolyte microreservoir (UPM) using two combinations of synthetic/synthetic (S/s; poly(allylamine hydrochloride) (PAH)/sodium poly (styrenesulfonate)) and synthetic/natural (S/n; PAH/sodium alginate) polyelectrolytes over spherical porous CaCO 3 core particles (CP) followed by core removal and to evaluate its biocompatibility and integrity of loaded model protein bovine serum albumin (BSA). A novel process for synthesis of CP was developed to obtain maximum yield of monodisperse vaterite (spherical) polymorph. The prepared UPM was characterized for surface morphology, layer-by-layer growth, pay load efficiency, integrity of BSA, as well as viability and cell adhesion using murine J 774 macrophages (Φ). In vitro release profile revealed that both S/s and S/n UPM were able to provide sufficient diffusion barrier to release protein at physiological pH. It has been observed that S/n UPM are fully biocompatible due to obvious reason of using natural polymer. In a separate experiment, the S/s UPM surface was modified with pluronic F-68 to tune biocompatibility which provides evidences for safety and tolerability of the S/s UPM as well. In nutshell, the proposed system could successfully be used for the delivery of proteins, and moreover, the system can be tailored to impart desired properties at any stage of layering especially in terms of drug release and to retain the integrity of proteins.

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Immobilization of enzymes by multilayer microcapsules

Cited by 71 publications

References 4 publications

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Electrostatic Assembly with Poly(ferrocenylsilanes)

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

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