Immobilization of l-asparaginase on gold nanoparticles for novel drug delivery approach as anti-cancer agent against human breast carcinoma cells

Al-Dulimi, Ali G.; Al-Saffar, Ali Z.; Sulaiman, Ghassan M.; Khalil, Khalil A. A.; Khashan, Khawla S.; Al-Shmgani, Hanady S.; Ahmed, Elhadi M.

doi:10.1016/j.jmrt.2020.10.021

Cited by 61 publications

(43 citation statements)

References 43 publications

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“…The preparations were visualized by using a Cellomics ArrayScan HCS reader (Thermo Scientific, USA). Dye fluorescence intensity was measured through the application of the cell health profiling bioapplication module [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

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[Retracted] Preparation of Iron Oxide and Titania‐Based Composite, Core‐Shell Populated, Nanoparticulates Material by Two‐Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer Agents

Bahjat

Ismail

Sulaiman

et al. 2022

Bioinorganic Chemistry and Applications

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Iron oxide and titania-based composite nanoparticles (NPs) populated with core-shell structures, as part of the mixture of the monometallic NPs, were prepared in water medium by the two-fluence LASER ablation technique by applying 30 and 60 mJ/cm2 LASER energy irradiations. The prepared monometallics, composite, and core-shell NPs structures were confirmed from the XRD, TEM, and EDX analyses, followed by the FE-SEM and UV absorptions. Optically, the NPs exhibited an increase in the energy gap from 3.27 eV to 3.75 eV as LASER fluence increased from 30 mJ/cm2 to 60 mJ/cm2. The average NPs core size distributions for the core-shell material ranged at ∼70 nm with the shell thickness around 20 nm. The biggest NPs were of ∼170 nm size which were sparsely distributed. The magnetization behaviors of the NPs were also investigated using the vibrating sample magnetometer (VSM). The NPs showed antimicrobial activities against the pathogenic species: Escherichia coli and Staphylococcus aureus. The antimicrobial activities of the synthesized NPs, synthesized under the influence of magnetic fields, were found to be more potent than the NPs synthesized without the presence of any magnetic field. The NPs prepared under the influence of the magnetic fields also comparatively exhibited higher levels of cytotoxicity against lung cancer cell lines (A549) than the NPs prepared under no magnetic field’s influence by the similar energy level effects of the LASER fluence. The flow cytometry analyses confirmed the NPs’ cytotoxic impacts against the human lung cancer A549 cell lines through the initiation of apoptosis and promotion of the cell cycle arrest at the G1 phase of cell division. To further confirm the cytotoxic effects and the mechanism of the anticancer activity of the synthesized NPs against the A549 cell lines, several related parameters (cell viability, membrane permeability, nuclear intensity, and cytochrome-C release) were analyzed using the high-content screening (HCS) assay. The study suggested that the prepared NPs have potential as antimicrobial and also as anti-lung-cancer agents as tested in vitro. These NPs can also be part of combined chemotherapy in different oncological interventions, as well as a sonosensitizer in sonomagnetic heating-based therapy, especially for cancers.

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Section: Methodsmentioning

confidence: 99%

“…A slightly modified method was adopted to analyze the cell cycle [ 27 , 31 ]. In brief, overnight-seeded A549 cells in a 25 cm 2 flask, with a concentration of 1 × 10 5 cell ml −1 , were subjected to various concentrations of 24 hr external magnetic field-synthesized NPs (100, 200, and 400 µ g mL −1 ).…”

Section: Methodsmentioning

confidence: 99%

[Retracted] Preparation of Iron Oxide and Titania‐Based Composite, Core‐Shell Populated, Nanoparticulates Material by Two‐Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer Agents

Bahjat

Ismail

Sulaiman

et al. 2022

Bioinorganic Chemistry and Applications

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“…The morphology, size, and shape of synthesized silver nanoparticles were examined with a scanning electron microscope, which was characterized by a magnification power between x1000000_ x6 through this examination [21]…”

Section: Scanning Electron Microscope (Sem)mentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles from Alhagi graecorum Leaf Extract and Evaluation of Their Cytotoxicity and Antifungal Activity

Hawar

Al-Shmgani

Al-Kubaisi

et al. 2022

Journal of Nanomaterials

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Green synthesis of silver nanoparticles (AgNPs) using different plant parts has shown a great potential in medicinal and industrial applications. In this study, AgNPs were in vitro green synthesized using A. graecorum, and its antifungal and antitumoractivities were investigated. Scanning electron microscopy (SEM) image result indicated spherical shape of AgNPs with a size range of 22-36 nm indicated by using Image J program. The functional groups indicated by Fourier-transform infrared spectroscopy (FTIR) represented the groups involved in the reduction of silver ion into nanoparticles. Alhagi graecorum AgNPs inhibited MCF-7 breast cancer cell line growth in increased concentration depend manner, significant differences shown at 50, 100, and 150 μg/ml concentrations compared to the control. Strong antifungal activity against Candida species (C. albicans., C. glabrata, C. parapsilosis, C. tropicales, and C. krusei) was observed and the inhibition zone range from 14-22 mm at a concentration of 0.01 mmol/ml and from 17-27 mm at a concentration of 0.02 mmol/ml. Based on our findings, it is concluded that synthesized silver nanoparticles from A. graecorum can be used as a potential antitumor and antifungal agent for various therapeutical applications.

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“…Traditional physiochemical methods of SeNP synthesizing are cumbersome, nonecological, and require prerequisites such as high temperatures, toxic substances, reagents, precursors, and complicated procedures. Besides, in the conventional process, the form, size, and instability of the synthesized particle are the main limitations [10]. This creates a great desire to synthesize nanoparticles with harnessed size and nanometer morphology in an eco-friendly mode [11].…”

Section: Introductionmentioning

confidence: 99%

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

Dhanraj

Rajeshkumar

2021

Journal of Nanomaterials

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Selenium is a trace element in the human body present in various enzymes with antioxidant activities and several functional proteins. This study is aimed at synthesizing selenium nanoparticles using Brassica oleracea (broccoli) and characterizing and assessing the antioxidant and antimicrobial effectiveness against cariogenic microorganisms. UV-visible spectrum displayed a peak at 370 nm which confirms the formation of SeNPs. TEM images of synthesized selenium nanoparticles showed polydisperse nanoparticles, spherical. The size of the particles ranged from 10 to 25 nm, and the average particle size obtained was 15.2 ± 1.9 nm . SEM images of nanoparticles were spherical and ranged in size from 10 to 28 nm. The SeNPs showed effective antimicrobial activity against cariogenic pathogens. The SeNPs synthesized with Brassica oleracea extract can be incorporated in toothpaste, gums, and mouthwashes that are cost-effective and biocompatible and used for the prevention of dental caries.

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Immobilization of l-asparaginase on gold nanoparticles for novel drug delivery approach as anti-cancer agent against human breast carcinoma cells

Cited by 61 publications

References 43 publications

[Retracted] Preparation of Iron Oxide and Titania‐Based Composite, Core‐Shell Populated, Nanoparticulates Material by Two‐Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer Agents

[Retracted] Preparation of Iron Oxide and Titania‐Based Composite, Core‐Shell Populated, Nanoparticulates Material by Two‐Step LASER Ablation in Aqueous Media as Antimicrobial and Anticancer Agents

Green Synthesis of Silver Nanoparticles from Alhagi graecorum Leaf Extract and Evaluation of Their Cytotoxicity and Antifungal Activity

Anticariogenic Effect of Selenium Nanoparticles Synthesized Using Brassica oleracea

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