Immobilization of laccase on polymer grafted polytetrafluoroethylene membranes for biosensor construction

Tastan, Evren; Onder, Sakip; Kök, Fatma Neşe

doi:10.1016/j.talanta.2011.01.031

Cited by 23 publications

(14 citation statements)

References 29 publications

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“…In a related research, Misra et al observed same profile for free and immobilized enzyme but the immobilized laccase higher sensitivity to pH variation [29]. Similarly, Tastan immobilized laccase through covalent bonding [30]. Also, the immobilized laccase showed a broader profile for pH-activity than the free laccase as well as higher activity at pH 8-10,…”

Section: Characteristics Of the Activity Of Immobilized Laccasementioning

confidence: 78%

“…As seen, increasing the concentration of EDAC enhanced the activity of immobilized laccase. EDAC/carboxylic group ratio higher than 0.4, they observed no activity, which was attributed to a negative effect on the enzyme at high concentrations of the cross-linker [30]. In addition, Figure 2 shows that the immobilization activity of laccase increases rapidly when the initial concentration of laccase in the immobilization mixture increases up to 10 mg/mL, then increases steadily upon further increase in the laccase concentration.…”

Section: Accepted Manuscriptmentioning

confidence: 97%

“…et al observed a gradual increase in the activity of the immobilized enzyme with increasing initial enzyme concentration[30].…”

mentioning

confidence: 96%

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Pinewood nanobiochar: A unique carrier for the immobilization of crude laccase by covalent bonding

Naghdi

Taheran

Brar

et al. 2018

International Journal of Biological Macromolecules

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Section: Characteristics Of the Activity Of Immobilized Laccasementioning

confidence: 78%

Section: Accepted Manuscriptmentioning

confidence: 97%

See 1 more Smart Citation

Pinewood nanobiochar: A unique carrier for the immobilization of crude laccase by covalent bonding

Naghdi

Taheran

Brar

et al. 2018

International Journal of Biological Macromolecules

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“…As published, ligninolytic enzymes, characterized by biodegradative capabilities toward a broad spectrum of structurally different xenobiotic compounds owing to their highly oxidative and non‐specific enzyme systems, have been applied in wastewater treatment. In particular, laccase is prominent in the degradation of xenobiotics . This two‐species immobilization system developed here provides a cellulose‐degrading metabolic pathway and lignin‐degrading metabolic pathway together.…”

Section: Discussionmentioning

confidence: 99%

A novel two‐species whole‐cell immobilization system composed of marine‐derived fungi and its application in wastewater treatment

Guan

Yao

2013

J of Chemical Tech & Biotech

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BACKGROUND Mycelia pellet has been exploited as a biological carrier for whole‐cell immobilization due to its unique structural characteristics. An innovative two‐species whole‐cell immobilization system was developed initially, which was achieved simply by inoculating the marine‐derived fungus Pestalotiopsis sp. J63 spores into culture medium containing another fungus Penicillium janthinellum P1 pre‐grown mycelia pellets for 2 days. RESULTS The resulting co‐immobilization system exhibited stronger biocatalytic activity, broader spectrum of substrates and remained more stable in media when subjected to hostile environment than free mycelia. In order to examine the potential function and prospects of this co‐immobilization system, the treatment of paper mill effluent was applied as a practical example. Numerous insoluble fine fibers in the wastewater were successfully and rapidly biodegraded and removed using this novel co‐immobilization system. CONCLUSION Overall, the present study offers a simple and reproducible way to architect a two‐species whole‐cell immobilization system, which is highly promising for biochemical and industrial processes. © 2013 Society of Chemical Industry

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“…This shift in optimum pH is generally caused by the proton exchange properties of the immobilization matrix (Cho et al, 2008). The microenvironment surrounding the immobilized enzymes affects their apparent optimum values and anionic polymers, for example, were shown to shift the pH optimum of the enzyme to more basic values (Tastan et al, 2011). The biosensor still had considerable activity at pH 5, but its activity was almost completely hindered at pH 9.…”

Section: Effect Of Phmentioning

confidence: 99%

Construction of an oxygen detection-based optic laccase biosensor for polyphenolic compound detection

Bilir¹,

Weil²,

Lochead³

et al. 2016

Turk J Biol

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A fiber optic biosensor was constructed from Pleurotus ostreatus laccase for the detection of polyphenolic compounds. Laccase was immobilized on the surface of the commercially available fiber optic oxygen sensor spots by using 3-aminopropylsilanetriol, glutaraldehyde, and amino-modified carboxycellulose. A diffusion layer containing tetramethyl orthosilicate (TMOS), trimethoxymethylsilane (Tri-MOS), and polyvinyl alcohol (PVA) was added to the immobilized laccase layer. The consumption of oxygen as a result of laccase activity was monitored using a fiber optical measuring setup with catechol as a model substrate. The optimal enzyme amount was determined as 1.5 mg per 50 µL of enzyme layer mixture, and with one diffusion layer and at pH 6.9, optimum detection conditions were attained. The biosensors have high reproducibility, stability (at least 85 days if stored in PBS at 4 °C), and convenient measurement duration (ca. 25 min between two successive measurements). The biosensor was found to have a broad linear working range for catechol (40-600 µM) and to be applicable to a flow-through system. In summary, an easy-to-produce, reproducible, and stable laccase sensor with a broad linear working range was produced. The sensor has potential in the food industry as well as in environmental monitoring for the detection of phenolic compounds.

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Immobilization of laccase on polymer grafted polytetrafluoroethylene membranes for biosensor construction

Cited by 23 publications

References 29 publications

Pinewood nanobiochar: A unique carrier for the immobilization of crude laccase by covalent bonding

Pinewood nanobiochar: A unique carrier for the immobilization of crude laccase by covalent bonding

A novel two‐species whole‐cell immobilization system composed of marine‐derived fungi and its application in wastewater treatment

Construction of an oxygen detection-based optic laccase biosensor for polyphenolic compound detection

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