Immobilization of streptavidin on 4H–SiC for biosensor development

Williams, Elissa H.; Davydov, Albert V.; Motayed, Abhishek; Sundaresan, Siddarth; Bocchini, Peter; Richter, Lee J.; Stan, Gheorghe; Steffens, Kristen L.; Zangmeister, Rebecca A.; Schreifels, John A.; Rao, Mulpuri V.

doi:10.1016/j.apsusc.2012.02.137

Cited by 92 publications

(74 citation statements)

References 35 publications

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“…Subsequently, after the immobilization of biotin onto the cysteamine SAM, the contact angle decreased to 21.911 ∘ from 46.720 ∘ of the cysteamine SAM. The reason was that the ureido-and tetrahydrothiophene-rings of biotin are more hydrophilic than −NH 2 terminated cysteamine SAM [26]. The contact angle of biotin SAM was smaller than the previously reported one in study [27], and this may be due to the well-organized biotin SAM.…”

Section: Resultscontrasting

confidence: 47%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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Surface plasmon resonance (SPR) is a real-time, label-free, and high-sensitive detection technology. SPR has been widely used in many applications such as biomolecular interaction analysis, environmental monitoring, and medical diagnostics. However, conventional SPR sensor systems usually require expensive equipment and complicated optics. In this paper, we have demonstrated a rapid prototyping of angle-scanning SPR for bioanalytical investigation. Rapid prototyping was attained by utilizing the FDM (fused deposition modeling) based 3D (three-dimensional) printing technology. Two rotating platforms were employed to drive the laser source and photodiode, respectively. A temperature regulation unit was incorporated to maintain the system temperature in order to reduce the temperature effect. The proposed SPR rapid prototyping yielded a refractive index resolution of 6.4×10 −6 RIU (refractive index unit), and the biotin-avidin system validated the kinetics parameters measurement capability. The obtained results indicated that the FDM 3D printing has great potential for developing rapid-prototyping SPR system.

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Section: Resultscontrasting

confidence: 47%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

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“…Streptavidin is one of the most common model proteins used in biosensing studies 64 and its detection is often used as the initial proof-of-concept for new biosensor designs. 28 sensor surface can be given high streptavidin selectivity and affinity by functionalising it with biotin.…”

Section: Streptavidin Biochemistrymentioning

confidence: 99%

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Lowe

Sun

Zeimpekis

et al. 2017

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107

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a Field-Effect Transistor sensors (FET-sensors) have been receiving increasing attention for biomolecular sensing over the last two decades due to their potential for ultra-high sensitivity sensing, label-free operation, cost reduction and miniaturisation. Whilst the commercial application of FET-sensors in pH sensing has been realised, their commercial application in biomolecular sensing (termed BioFETs) is hindered by poor understanding of how to optimise device design for highly reproducible operation and high sensitivity. In part, these problems stem from the highly interdisciplinary nature of the problems encountered in this field, in which knowledge of biomolecular-binding kinetics, surface chemistry, electrical double layer physics and electrical engineering is required. In this work, a quantitative analysis and critical review has been performed comparing literature FET-sensor data for pH-sensing with data for sensing of biomolecular streptavidin binding to surface-bound biotin systems. The aim is to provide the first systematic, quantitative comparison of BioFET results for a single biomolecular analyte, specifically streptavidin, which is the most commonly used model protein in biosensing experiments, and often used as an initial proofof-concept for new biosensor designs. This novel quantitative and comparative analysis of the surface potential behaviour of a range of devices demonstrated a strong contrast between the trends observed in pH-sensing and those in biomolecule-sensing. Potential explanations are discussed in detail and surfacechemistry optimisation is shown to be a vital component in sensitivity-enhancement. Factors which can influence the response, yet which have not always been fully appreciated, are explored and practical suggestions are provided on how to improve experimental design.

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“…We present a solution based sequential layer functionalization technique for specific streptavidin (SA) protein immobilization on Si NWs. This functionalization technique combines the protocol for selective SA immobilization to SiC planar surfaces 5 with the "in-suspension" biofunctionalization of NWs developed for the attachment of DNA to Au NWs. 6 An advantage of this technique is the ability to functionalize separate NW batches for the specific attachment of different types of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionalization of Si nanowires using a solution based technique

et al. 2012

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Here we present a solution based functionalization technique for streptavidin (SA) protein conjugation to silicon nanowires (Si NWs). Si NWs, with a diameter of 110 nm to 130 nm and a length of 5 µm to 10 µm, were functionalized with 3-aminopropyltriethoxysilane (APTES) followed by biotin for the selective attachment of SA. High-resolution transmission electron microscopy (HRTEM) and atomic force microscopy (AFM) showed that the Si NWs were conformally coated with 20 nm to 30 nm thick APTES, biotin, and SA layers upon functionalization. Successful attachment of each bio/organic layer was confirmed by X-ray photoelectron spectroscopy (XPS) and fluorescence microscopy. Fluorescence microscopy also demonstrated that there was an undesirable non-specific binding of the SA protein as well as a control protein, bovine serum albumin (BSA), to the APTES-coated Si NWs. However, inhibition of BSA binding and enhancement of SA binding were achieved following the biotinylation step. The biofunctionalized Si NWs show potential as label-free biosensing platforms for the specific and selective detection of biomolecules.

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Immobilization of streptavidin on 4H–SiC for biosensor development

Cited by 92 publications

References 35 publications

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Field-effect sensors – from pH sensing to biosensing: sensitivity enhancement using streptavidin–biotin as a model system

Biofunctionalization of Si nanowires using a solution based technique

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