Immobilization of urease onto chemically modified acrylonitrile copolymer membranes

Catalytic performances of chemically immobilized urease under static and dynamic conditions: A comparative study

“…For native urease, the optimum temperature was found to be 37 • C whereas, it was shifted to 60 • C for immobilized urease. Similar results have been reported for the immobilized urease on different supports with different immobilization methods [10,11,41]. The results in Fig.…”

“…Furthermore, removal rate of urea from a dialyzer which consists of urease immobilized membrane was measured 2 times faster than the rate in a regular dialyzer. Godjevargova and Gabrovska [10] immobilized urease covalently onto poly(acrylonitrile-methylmethacrylatesodium vinylsulfonate) membranes subjected to seven different chemical modifications. The influences of modifications on pH optimum, water flow through the membrane, reaction rates, thermal inactivation and storage stabilities were investigated.…”

New amperometric glucose biosensor based on cross-linking of glucose oxidase on silica gel/multiwalled carbon nanotubes/polyacrylonitrile nanocomposite film

“…In our study we have focused our attention on polyacrylonitrile (PAN), because of its good film forming properties, insolubility in water solutions and the good mechanical stability for long period of time. It has not active groups for enzyme immobilization [19], but chemically modified PAN is very suitable for this purpose [20][21][22][23]. Addition of silica gel in polymer film of modified PAN provides greater specific surface of the electrode and higher enzyme loading.…”

The influence of the support nature on the kinetics parameters, inhibition constants and reactivation of immobilized acetylcholinesterase

“…A few publications deal with covalent immobilization of enzymes onto chemically modified membranes of AN copolymer [11]. Among immobilization techniques, covalent attachment has a higher commercial potential because it ensures more time stability with a longer retention of catalytic activity.…”

“…The charge of the carrier is of a great importance for enzyme kinetics [11]. The matrix to which enzyme is ultimately attached, indeed, has an important influence on the properties of the enzyme such as activity, reaction rate, substrate specificity, inhibitors action, kinetic constants, etc.…”