Immobilization of xylanase on differently functionalized silica gel supports for orange juice clarification

Alagöz, Dilek; Varan, Nazlı Ece; Toprak, Ali; Yıldırım, Deniz; Tükel, S. Seyhan; Fernández‐Lafuente, Roberto

doi:10.1016/j.procbio.2021.12.027

Cited by 35 publications

(10 citation statements)

References 81 publications

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“…Silica has several benefits, including a large surface area and good mechanical and thermal stability. Also, by interacting with organofunctionalized silanes groups, it is simple to modify and functionalize the structure [ 14 , 15 ]. These covalently bonded chemical groups serve as a highly stable long arm spacer, allowing the receptor to make contact with the copper ion and then TMP.…”

Section: Introductionmentioning

confidence: 99%

Experimental and theoretical study for removal of trimethoprim from wastewater using organically modified silica with pyrazole-3-carbaldehyde bridged to copper ions

et al. 2022

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Background Human and veterinary antibiotics are typically discharged as parent chemicals in urine or feces and are known to be released into the environment via wastewater treatment plants (WWTPs). Several research investigations have recently been conducted on the removal and bioremediation of pharmaceutical and personal care products (PPCPs) disposed of in wastewater. Results SiNP-Cu, a chelating matrix, was produced by delaying and slowing 1.5-dimethyl-1H-pyrazole-3-carbaldehyde on silica gel from functionalized with 3-aminopropyltrimethoxysilane. The prepared sorbent material was characterized using several techniques including BET surface area, FT-IR spectroscopy, Scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and nitrogen adsorption–desorption isotherm. The pseudo-second-order model provided the best correlation due to the big match between the experimental and theoretical of different adsorption coefficients. The Langmuir and Freundlich adsorption models were used and the study showed a better match with the Freundlich model with a capacity of removal reached up to 420 mg g−1. The removal capacity was dependent on pH and increased by increasing pH. The removal percentage reached 91;5% at pH = 8. The adsorbent demonstrated a high percentage removal of TMP, reaching more than 94% when increased pH. The sample was simply regenerated by soaking it for a few minutes in 1 N HCl and drying it. The sorbent was repeated five times with no discernible decrease in removal capacity. The thermodynamic study also showed endothermic, increasing randomness and not spontaneous. The free energy was 2.71 kJ/mol at 320 K. The findings of the DFT B3LYP/6–31 + g (d, p) local reactivity descriptors revealed that nitrogen atoms and π-electrons of the benzene and pyrimidine rings in the TMP are responsible for the adsorption process with the SiNP surface. The negative values of the adsorption energies obtained by molecular dynamic simulation indicated the spontaneity of the adsorption process. Conclusion The global reactivity indices prove that TMP is stable and it can be removed from wastewater using SiNP surface. The results of the local reactivity indices concluded that the active centers for the adsorption process are the nitrogen atoms and the π-electrons of the pyrimidine and benzene rings. Furthermore, the positive value of the maximum charge transfer number (ΔN) proves that TMP has a great tendency to donate electrons to SiNP surface during the process of adsorption.

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Section: Introductionmentioning

confidence: 99%

Experimental and theoretical study for removal of trimethoprim from wastewater using organically modified silica with pyrazole-3-carbaldehyde bridged to copper ions

et al. 2022

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“…Therefore, the lemon peel powder was more effective than commercial pectinase and could be suggested as naturally immobilized pectinase source (due to its reusability up to 5 cycles) for producing the clear juice. In another study, the xylanase from Trichoderma longibrachiatum immobilized via ion exchange on differently functionalized silica gel supports was employed for orange juice clarification using a batch type reactor by Alagöz et al (2022). The supports included: (1) xylanase immobilized ionically on 3-propylsulfonic acid-functionalized silica gel (Xyl/Si-SO 3 ), (2) xylanase immobilized ionically on 3-aminopropyl-functionalized silica gel (Xyl/Si-NH 3 ), (3) xylanase immobilized covalently on 3-carboxypropylfunctionalized silica gel (Xyl/Si-COOH), and (4) xylanase immobilized covalently on glutaraldehyde-activated 3aminopropyl-functionalized silica gel (Xyl/Si-Glu).…”

Section: Immobilized Enzymesmentioning

confidence: 99%

Application of natural fining agents to clarify fruit juices

Shirvani,

Mirzaaghaei,

Goli

2023

Comp Rev Food Sci Food Safe

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The consumption of fruit juices has been increasingly growing all over the world. The clarification process is considered as one of the most important stages in fruit juice production, which can provide the products with desired clear visual appearance. Nowadays, the tendency of consumers to use the natural‐clarified fruit juices encourages the researchers to allocate much attention on utilization of natural clarifying agents to clarify different fruit juices. This review article has first introduced the most frequent causes of turbidity in fruit juices including polysaccharides (i.e., cellulose, hemicelluloses, lignin, starch, and pectic substances), proteins and polyphenols (especially tannins) as well as their removal mechanisms. After that, a comprehensive summary of research on natural fining agents, including clay minerals, polysaccharides, proteins, enzymes (free and immobilized forms), and activated carbon is provided with a focus on their application in the juice clarification process. The chemical composition of natural substances, their efficiency on reduction of turbidity‐causing compounds and the changes in properties of clarified juices such as turbidity (clarity), total phenolic content, total anthocyanins, viscosity, and sensory evaluation followed by their stability during the storage have been deeply discussed.

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“…Figure 7B shows that IMA-XY lost about half of its activity after continuing incubation at the same temperature for up to 75 min. Alagoz et al [42] covalently immobilized XY on functionalized silica with a residual activity value of 77.2% after 24 h of incubation at 50 • C. The activity of FMA-XY decreased rapidly after 45 min of incubation at 60 • C to 20.05% of its initial activity. In contrast, the relative enzyme activity of IMA-XY remained at 38.58% of the initial activity when kept at 60 • C for 75 min.…”

Section: Mechanical Analysis Of Thermal Inactivitymentioning

confidence: 99%

Thermodynamics and Physicochemical Properties of Immobilized Maleic Anhydride-Modified Xylanase and Its Application in the Extraction of Oligosaccharides from Wheat Bran

Zhao

Guo

et al. 2023

Foods

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Xylanases are the preferred enzymes for the extracting of oligosaccharides from wheat bran. However, free xylanases have poor stability and are difficult to reuse, which limit their industrial application. In the present study, we covalently immobilized free maleic anhydride-modified xylanase (FMA-XY) to improve its reusability and stability. The immobilized maleic anhydride-modified xylanase (IMA-XY) exhibited better stability compared with the free enzyme. After six repeated uses, 52.24% of the activity of the immobilized enzyme remained. The wheat bran oligosaccharides extracted using IMA-XY were mainly xylopentoses, xylohexoses, and xyloheptoses, which were the β-configurational units and α-configurational units of xylose. The oligosaccharides also exhibited good antioxidant properties. The results indicated that FMA-XY can easily be recycled and can remain stable after immobilization; therefore, it has good prospects for future industrial applications.

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Immobilization of xylanase on differently functionalized silica gel supports for orange juice clarification

Cited by 35 publications

References 81 publications

Experimental and theoretical study for removal of trimethoprim from wastewater using organically modified silica with pyrazole-3-carbaldehyde bridged to copper ions

Experimental and theoretical study for removal of trimethoprim from wastewater using organically modified silica with pyrazole-3-carbaldehyde bridged to copper ions

Application of natural fining agents to clarify fruit juices

Thermodynamics and Physicochemical Properties of Immobilized Maleic Anhydride-Modified Xylanase and Its Application in the Extraction of Oligosaccharides from Wheat Bran

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