Immobilized Forms of the Ophiostoma piceae Lipase for Green Synthesis of Biodiesel. Comparison with Eversa Transform 2.0 and Cal A

Molina-Gutiérrez, María; Alcaraz, Lorena; López, Félix A.; Rodríguez-Sánchez, Leonor; Martı́nez, Marı́a Jesús; Prieto, Alicia

doi:10.3390/jof7100822

Cited by 7 publications

(5 citation statements)

References 69 publications

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“…Furthermore, water in the reaction system may inhibit the positive progression of the lipase-catalyzed reaction and result in the high acid value of the product ( Hájek et al, 2012 ; Nguyen et al, 2018 ). During the reaction, the surface of the lipase Eversa ® Transform 2.0 requires a layer of essential water to maintain a certain active conformation for its catalytic activity ( Molina-Gutiérrez et al, 2021 ). Therefore, the dehydration capacity of the water removal agent should be appropriate, so that not only the acid value of the FAEE product is desired, but also an essential layer of water is not taken away from the surface of the enzyme.…”

Section: Resultsmentioning

confidence: 99%

Decreasing acid value of fatty acid ethyl ester products using complex enzymes

Li,

Guo,

Sun

2024

Front. Bioeng. Biotechnol.

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Recently, enzymatic method has been used to prepare biodiesel using various oils. But the high acid value of the biodiesel product using enzyme as a catalyst has been one issue. In this work, an attempt to reduce the acid value of fatty acid ethyl ester (FAEE) product to satisfy the specified requirement (AV ≤ 0.5 mgKOH/g), a complex enzyme-catalyzed method was used for the ethanolysis of Semen Abutili seed oil (SASO) (AV = 5.5 ± 0.3 mgKOH/g). The effects of various variables (constituents of complex enzyme, type and addition of water removal agent, time, temperature, enzyme addition load, substrate ratio) on the enzymatic reaction were investigated. The optimal reaction conditions were: 1% addition of liquid lipase Eversa® Transform 2.0% and 0.8% of enzyme dry powder CALB, reaction temperature 35°C, alcohol-oil ratio 9:1 (mol/mol), 0.8 g/g of 4A-MS and reaction time 24 h. Under the optimal reaction conditions, the FAEE yield was 90.8% ± 1.5% and its acid value was decreased from 12.0 ± 0.2 mgKOH/g to 0.39 ± 0.10 mgKOH/g. In further evaluating the feasibility of preparing FAEE from SASO, the FAEE products obtained under the optimal reaction conditions were purified and evaluated with reference to the ASTM D6751 standard for the main physicochemical indexes. The results obtained were in accordance with the requirements except for the oxidative stability.

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Section: Resultsmentioning

confidence: 99%

Decreasing acid value of fatty acid ethyl ester products using complex enzymes

Li,

Guo,

Sun

2024

Front. Bioeng. Biotechnol.

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“…The recognition of filamentous fungi with hydrolytic enzymatic activity suggests that they are preferred sources for the industry, due to superior metabolic versatility ( Su et al 2012 ). Fungal enzymes are used in biofuel production processes, bioremediation of organic contaminants, for detergent production, antibiotic production, bleaching of textile fabrics, juice clarification, removal of leather aromas and paper production ( El-Sawah et al 2021 , Füting et al 2021 , Hyde et al 2019 , Kirk et al 2002 , Meyer et al 2020 , Molina-Gutiérrez et al 2021 , Niyonzima 2020 , Singh et al 2021 , Sudeep et al 2020 , Syedd-León et al 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…From a social and economic perspective, these microorganisms play an important role in the pharmaceutical, agricultural, food, detergent, leather, paper industries and biofuel ( El-Sawah et al 2021 , Hyde et al 2019 , KC et al 2020 , Meyer 2021 , Molina-Gutiérrez et al 2021 , Singh et al 2021 ), for the generation of hydrolytic enzymes, which can be used in novel biotechnological processes ( Ramos Sanchez 2015 , Syedd-León et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Gómez-Vázquez,

Sánchez Roque,

Ibáñez-Duharte

et al. 2024

BDJ

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Filamentous fungi, microorganisms that develop and are located in different habitats, are considered important producers of enzymes and metabolites with potential for the biotechnology industry. The objective of this work was to isolate and identify filamentous fungi that grow in used oil. Two fungal species were characterised through their morphology and molecular identification. The DNA of each extracted strain was amplified by PCR using primers ITS1 and ITS4, obtaining sequences that were later in GenBank (NCBI). A white coloured strain (HB) with a cottony, white, hyaline morphology and irregular borders was observed; so too, a brown colony (HC) with a sandy surface, a well-defined border of beige colour in early growth until it became a dark brown colour. The identity result by homology of the sequences in the BLASTn database was 100% and 99.55%, indicating that they correspond to Cladosporium tenuissimum and Fomitopsis meliae, respectively. Finally, the results in lipolytic activity show greater potential for Fomitopsis meliae with 0.61 U/l in residual oil. Thus, it is important to highlight the potential of this type of waste to favour the prospection of microorganisms for a sustainable alternative for future studies of biological conversion.

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“…In that study, the outstanding catalytic stability of the mentioned catalyst was discovered even after the fifth reused cycle and is capable of achieving a fatty acid methyl ester (FAME) yield of around 93%. Other than heterogeneous catalysts, biocatalysts such as immobilized lipase from Bacillus mycoides and Ophiostoma piceae strains, as well as fermented macaúba cake are used to assist in enzymatic transesterification [18][19][20]. Biocatalysts are studied as they are eco-friendly, generate minimal waste while consuming less energy, and function well under mild process conditions [20].…”

Section: Renewable Diesel and Biodiesel Productionmentioning

confidence: 99%

Indigenous Materials as Catalyst Supports for Renewable Diesel Production in Malaysia

Chia

Nomanbhay²,

Chew³

et al. 2022

Energies

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High energy demand from the market due to the rapid increment of the human population worldwide has urged society to explore alternatives to replace non-renewable energy. Renewable diesel produced from biomass could be the next potential energy source for its high stability, long-term storage, and comparable performance with diesel fuels. In producing renewable diesel, the application of catalyst is essential, and the catalyst support is synthesized with the catalyst to enhance the reaction rate and catalytic properties. In this review, the type of catalyst support will be reviewed along with a brief introduction to biodiesel and renewable diesel production, especially focusing on zeolites as the catalyst support. The enhancement of catalyst support will be critically discussed to improve the catalytic performance of support in renewable diesel production and important aspects such as the stability and recyclability of the supported catalyst are included. The application of the supported catalyst in increasing the selectivity and yield of renewable diesel is significant, in which the catalytic properties depend on the interaction between catalyst and catalyst support. The supported catalyst as a favorable substance to assist in enhancing renewable diesel yield could lead to a sustainable and greener future for the biofuel industry in Malaysia.

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Immobilized Forms of the Ophiostoma piceae Lipase for Green Synthesis of Biodiesel. Comparison with Eversa Transform 2.0 and Cal A

Cited by 7 publications

References 69 publications

Decreasing acid value of fatty acid ethyl ester products using complex enzymes

Decreasing acid value of fatty acid ethyl ester products using complex enzymes

Molecular identification and lipolytic potential of filamentous fungi isolated from residual cooking oil

Indigenous Materials as Catalyst Supports for Renewable Diesel Production in Malaysia

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