2008
DOI: 10.1365/s10337-008-0727-y
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Immobilized Metal Affinity Chromatography of Phosphorylated Proteins Using High Performance Sorbents

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Cited by 7 publications
(3 citation statements)
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“…Immobilized metal ion affinity chromatography (IMAC) has been widely used to capture the molecules containing phosphate group such as phosphopeptides via the affinity of the phosphate groups to metal ions [21][22][23][24][25][26]. Recently, it has been reported that a thin polydopamine (PD) layer can adhere to the surface of IMAC substrates by the polymerization of dopamine, which not only increases the hydrophility of IMAC materials but also provides numerous adjacent hydroxide groups as the binding sites for metal ions [27][28][29].…”
Section: Introductionmentioning
confidence: 99%
“…Immobilized metal ion affinity chromatography (IMAC) has been widely used to capture the molecules containing phosphate group such as phosphopeptides via the affinity of the phosphate groups to metal ions [21][22][23][24][25][26]. Recently, it has been reported that a thin polydopamine (PD) layer can adhere to the surface of IMAC substrates by the polymerization of dopamine, which not only increases the hydrophility of IMAC materials but also provides numerous adjacent hydroxide groups as the binding sites for metal ions [27][28][29].…”
Section: Introductionmentioning
confidence: 99%
“…15,16 Much effort has been devoted to develop IMAC materials, in which the metal ions are immobilized to different adsorbents (polymer beads, porous materials or nanoparticles) via acidic chelating ligands of iminodiacetic acid (IDA), phosphate group or nitrilotriacetic acid (NTA). [16][17][18][19][20][21][22][23] However, these conventional IMAC techniques often require centrifugations at high speed during sample preparation, which are not only laborious and inconvenient, but also lead to the undesirable loss of low-abundance phosphopeptides. 24 On-plate strategy for the purification of phosphopeptides would reduce sample loss, but they are not suitable for the high-throughput phosphoproteome analysis of complicated samples, such as whole cell lysates.…”
Section: Introductionmentioning
confidence: 99%
“…To date, various materials and techniques have recently been introduced for the selective enrichment of phosphopeptides. Immobilized metal ion affinity chromatography (IMAC), which relies on the affinity of the phosphate groups to metal ions, is the most widely used technique to capture the phosphopeptides out of the pool of predominantly nonphosphopeptides, in which the metal ions are immobilized to different adsorbents via acidic chelating ligands of iminodiacetic acid (IDA) or nitrilotriacetic acid (NTA). However, the bound metal ions were easily lost during sample loading and washing caused by the relatively weaker interaction (each metal ion only coordinates one IDA or NTA ligand). To solve this problem, Zou et al recently introduced a new ligand of phosphate group to immobilize Ti 4+ or Zr 4+ for phosphoproteome research. , Although IMAC technique has been highly developed, it still has some drawbacks.…”
mentioning
confidence: 99%