Immune Niche Within the Peritoneal Cavity

Okabe, Yasutaka

doi:10.1007/978-3-030-86016-5_6

Cited by 7 publications

(11 citation statements)

References 53 publications

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“…Host immunity plays crucial roles in the process of metastasis formation (10,11). Previous studies have shown that the peritoneal cavity usually contains many free-floating immune cells of various phenotypes which sustain peritoneal homeostasis and prevent local inflammation (12,13). Since they can mediate direct contact with tumor cells exfoliated from the serosal surface of primary tumors, these immune cells in the peritoneal fluids are thought to play critical roles in the pathophysiology of PM.…”

Section: Introductionmentioning

confidence: 99%

Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer

Takahashi

Kurashina²,

Yamaguchi³

et al. 2022

Front. Immunol.

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BackgroundThe peritoneal cavity contains many site-specific immune cells which constitute a unique immune microenvironment. However, it is unclear how the local immune signature is altered in patients with peritoneal metastases (PM).MethodsPeritoneal lavage fluid or ascites were obtained from 122 patients with various stages of gastric cancer (GC). Cells recovered from peritoneal fluids were immunostained with mAbs for lymphocyte-, macrophage- and tumor cell-specific antigens and the frequencies of leukocyte subsets and antigen expression levels were evaluated with multi-color flowcytometry.ResultsThe proportions of CD8(+) T cells, CD3(+)CD56(+) NKT-like cells, and CD3(-)CD56(+) NK cells to CD45(+) leukocytes were significantly reduced in patients with PM compared to those without PM. In patients with PM, the rates of CD8 (+) T cells and NKT-like cells correlated inversely with the tumor leukocyte ratio (TLR), the relative frequency of CD326(+) tumor cells to CD45(+) leukocytes. In contrast, the proportion of CD19(+) B cells was significantly increased in patients with PM, and their proportion correlated positively with the TLR and peritoneal carcinomatosis index (PCI) score. In patients with PM, CD14(+) macrophages tended to be increased with enhanced expression of CD14, CD16 and a M2-macrophage marker, CD163. In particular, macrophages in patients with high TLR contained many granules with high side scatter and CD14 expression in their flow profile compared to those without PM.ConclusionPM are accompanied by a drastic change in phenotypes of lymphocyte and macrophage in the peritoneal cavity, which might be involved in the development and progression of intraperitoneal tumor growth.

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Section: Introductionmentioning

confidence: 99%

Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer

Takahashi

Kurashina²,

Yamaguchi³

et al. 2022

Front. Immunol.

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“…Next, we could observe inflammatory response modulation by EE in peripheral tissues (PC and spleen) and lymphocytes. The PC has a visceral lining that is highly sensitive to various internal and external stimuli influencing the immune system activity [ 36 ]. Our analysis demonstrates a discrepancy in the expression of pro-inflammatory cytokines in mice PC after an EE protocol.…”

Section: Resultsmentioning

confidence: 99%

Cytokines and Immune Cells Profile in Different Tissues of Rodents Induced by Environmental Enrichment: Systematic Review

Fernandes

Santos

Filgueira

et al. 2022

IJMS

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Environmental Enrichment (EE) is based on the promotion of socio-environmental stimuli, which mimic favorable environmental conditions for the practice of physical activity and health. The objective of the present systematic review was to evaluate the influence of EE on pro-and anti-inflammatory immune parameters, but also in cell activation related to the innate and acquired immune responses in the brain and peripheral tissues in rodents. Three databases [PubMed (2209 articles), Scopus (1154 articles), and Science Direct (1040 articles)] were researched. After applying the eligibility criteria, articles were selected for peer review, independently, as they were identified by September 2021. The protocol for this systematic review was registered in the PROSPERO. Of the 4417 articles found, 16 were selected for this systematic review. In the brain, EE promoted a reduction in proinflammatory cytokines and chemokines. In the blood, EE promoted a higher percentage of leukocytes, an increase in CD19+ B lymphocytes, and the proliferation of Natura Killer (NK cells). In the bone marrow, there was an increase in the number of CD27− and CD11b+ mature NK cells and a reduction in CD27− and CD11b+ immature Natural Killer cells. In conclusion, EE can be an immune modulation approach and plays a key role in the prevention of numerous chronic diseases, including cancer, that have a pro-inflammatory response and immunosuppressive condition as part of their pathophysiology.

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“…Notably, immune evasion by disseminated tumor cells within the peritoneal milieu is recognized as a critical event in the progression of PM. The peritoneal cavity harbors a distinctive immune environment that markedly differs from the systemic circulation 11 , 12 . There are many free-floating immune cells with various phenotypes which sustain peritoneal homeostasis and prevent local inflammation 11 , 13 , 14 .…”

Section: Introductionmentioning

confidence: 99%

“…The peritoneal cavity harbors a distinctive immune environment that markedly differs from the systemic circulation 11 , 12 . There are many free-floating immune cells with various phenotypes which sustain peritoneal homeostasis and prevent local inflammation 11 , 13 , 14 . Importantly, alterations in the phenotype of these immune cells have been reported in patients with PM 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Importantly, alterations in the phenotype of these immune cells have been reported in patients with PM 15 . Within this context, "milky spot" cellular aggregates featuring a central core of B cells enveloped by T cells, myeloid cells, and fibroblastic stromal cells embedded in adipocytes beneath the mesothelial cell layer in the omentum 16 , 17 , play a central role in regulating local immune responses to inflammatory stimuli and cancer 11 , 14 , 18 . Intriguingly, milky spots are known to serve as primary implantation sites for tumor cells in PM, implying that immune cells within milky spots may predominantly exert immunosuppressive functions 19 – 22 .…”

Section: Introductionmentioning

confidence: 99%

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Vagus nerve signal has an inhibitory influence on the development of peritoneal metastasis in murine gastric cancer

Futoh,

Miyato,

Yamaguchi

et al. 2024

Sci Rep

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The vagus nerve is the only pathway for transmitting parasympathetic signals between the brain and thoracoabdominal organs, thereby exhibiting anti-inflammatory functions through the cholinergic anti-inflammatory pathway. Despite often being resected during lymph node dissection in upper gastrointestinal cancer surgery, the impact of vagotomy on postoperative outcomes in gastric cancer patients remains unclear. Sub-diaphragmatic vagotomy was performed on C57BL/6 mice. Three weeks later, syngeneic murine gastric cancer cell line YTN16P was injected into the peritoneal cavity, and the number of peritoneal metastases (PM) on the mesentery and omentum compared with control mice. The phenotypes of immune cells in peritoneal lavage and omental milky spots one day after tumor inoculation were analyzed using flow cytometry and immunohistochemistry. Intraperitoneal transfer of 3 × 105 YTN16P significantly increased the number of metastatic nodules on the mesentery in the vagotomy group compared to the control group. The omental metastasis grade was also significantly higher in the vagotomy group. Phenotypic analysis of immune cells in peritoneal lavage did not reveal significant differences after vagotomy. However, vagotomized mice exhibited a notable increase in milky spot area, with a higher presence of cytokeratin(+) tumor cells, F4/80(+) macrophages, and CD3(+) T cells. Vagus nerve signaling appears to regulate the immune response dynamics within milky spots against disseminated tumor cells and inhibits the development of PM. Preserving the vagus nerve may offer advantages in advanced gastric cancer surgery to reduce peritoneal recurrence.

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Immune Niche Within the Peritoneal Cavity

Cited by 7 publications

References 53 publications

Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer

Altered intraperitoneal immune microenvironment in patients with peritoneal metastases from gastric cancer

Cytokines and Immune Cells Profile in Different Tissues of Rodents Induced by Environmental Enrichment: Systematic Review

Vagus nerve signal has an inhibitory influence on the development of peritoneal metastasis in murine gastric cancer

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