Immune Regulation in Neoplasia

Steele, J.; Chan, Agnes; Stammers, A T; Levy, Julia G.; Singhai, Rakesh

doi:10.1007/978-1-4613-1925-2_18

Development and Recognition of the Transformed Cell 1987

DOI: 10.1007/978-1-4613-1925-2_18

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Immune Regulation in Neoplasia

J. Steele

Agnes Chan

A T Stammers

et al.

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1988

1990

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Cited by 2 publications

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“…This controversy may relate to the fact that at least two types of antigen-specific Ts subsets exist [in the 4-hydroxy-3-nitrophenyl (NP) acetyl hapten system they are referred to as inducer or Ts1 cells and effector or Ts3 cells] (14). Since Ts cells were first discovered, many groups have made hybridoma lines that correlate with the Ts1 or Ts3 populations with respect to function, genetic restriction, and specificity (14)(15)(16)(17)(18)(19)(20). The antigen-specific receptors of these hybridomas have not been studied, but many hybridomas have been probed for the genes of the conventional ac receptor.…”

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confidence: 99%

Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Kuchroo¹,

Steele²,

Billings³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Three suppressor T (Ts)-cell hybridomas specific for 4-hydroxy-3-nitrophenyl acetyl (NP) hapten were selected for surface expression of cluster determinant 3 (CD3) by using antibody (anti-CD3) or antigen (NP-bovine serum albumin) panning procedures followed by cloning at limiting dilution. The CD3-selected Ts hybridomas showed a 1-2 logarithmic enrichment in suppressor activity when compared to the parent lines; they also specifically bound NP-coupled sheep red blood cells in rosette assays. This antigen-binding ability could be down-modulated by anti-CD3 antibody. Similarly, surface expression of CD3 was specifically downmodulated by preincubation of these hybridomas with antigen. Anti-CD3 monoclonal antibody under reducing conditions coprecipitated a broad band of 38-50 kDa associated with two CD3 (25 and 16 kDa) bands. T-cell receptor, anti-a-specific monoclonal antibody also immunoprecipitated a broad band in the 41 to 49-kDa region. The combined results suggest that, like helper and cytotoxic T lymphocytes, Ts cells also bear antigenspecific receptors associated with CD3 molecules.The structure and biochemical nature ofthe antigen receptors on helper and cytotoxic T cells have been extensively characterized. In both cases, the receptor is a disulfide-linked heterodimeric glycoprotein containing an a and a f chain of "40-43 kDa (1, 2). The T-cell antigen receptor is noncovalently associated with several cluster determinant 3 (CD3) polypeptide chains, thus making a T-cell receptor (TcR)-CD3 complex (1,(3)(4)(5). By using anti-CD3 monoclonal antibodies (mAbs), the functional association of CD3 with the T-cell antigen receptor has been demonstrated in several ways; antibodies directed to CD3 can either stimulate (6) or inhibit (7, 8) T-cell activation and anti-CD3 antibodies can coimmunoprecipitate TcR (6,9).In contrast, the antigen recognition structures utilized by antigen-specific suppressor T (Ts) cells remain poorly characterized, although recent reports have described the existence of a/B structures on leprosy-specific human Ts cell clones (10) and transformed lysozyme-specific Ts cells (11).Other reports suggest that these receptors are not relevant in Ts cells (12,13 hybridomas-namely CKB-Ts3-3, CKB-Ts3-9, -and B6-Ts3-8-were used during the present study. In addition, the B6-Ts1-29 and CKB-Ts1-17 hybridomas were used for the

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mentioning

confidence: 99%

Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Kuchroo¹,

Steele²,

Billings³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

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Comparison of T suppressor factors from tumour-bearing mice and mice immunized with a monoclonal anti-idiotypic antibody

Greer

Halliday

1990

Cancer Immunol Immunother

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Certain dosage schedules of a monoclonal anti-idiotypic antibody (related to a murine bladder carcinoma) were found to induce suppressor factor production by syngeneic mice. This suppressor factor resembled the factor from tumour-bearing mice with respect to idiotype specificity, possession of molecular markers (reactive with anti-IJ and B16G antibodies) and production by Lyt2+IJ+ T cells in spleen cell cultures. The two factors differed with respect to Igh restriction in an in vitro assay (leucocyte adherence inhibition) and ability to suppress the induction of delayed-type hypersensitivity to tumour antigen.

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Immune Regulation in Neoplasia

Cited by 2 publications

References 33 publications

Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Expression of CD3-associated antigen-binding receptors on suppressor T cells.

Comparison of T suppressor factors from tumour-bearing mice and mice immunized with a monoclonal anti-idiotypic antibody

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