Immune response of cattle to vaccination with Brucella abortus strain 19 in India

Baksi, Surajit; Dave, Hiral; Rao, Nirav; Oza, R; Khan, Mukandar

doi:10.3329/bvet.v33i1.33311

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…The antibody titer has been reported to be significantly increased in the vaccinated calves after one month and the titer declined but remained positive up to six months and then negative throughout the 12 months study periods. 38 These observations could not be compared with the present study because this study did not continue after 150 days of vaccination.…”

Section: Discussionmentioning

confidence: 70%

Humoral Immune Response in Cross-Bred Heifers Immunized With Brucella Abortus Strain Rb51 Vaccine in Military Dairy Farm of Bangladesh

Rahman¹,

Rahman²,

Rahman³

et al. 2020

JVMOHR

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Background: Brucellosis is a chronic zoonotic disease with negligible mortality rate that might be the reason not to attract the concerned authority to prevent and eradicate it in low income endemic countries. Recently, it has been recognized as a re-emerging zoonotic disease not only in low income countries but also its eradicated developed world. Objective: The main objective was to determine the humoral immune response (HIR) in crossbred dairy heifers immunized with Brucella abortus strain RB51 vaccine by using indirect ELISA Materials and Methods: Each of the 20 randomly selected B. abortus sero-negative crossbred (Holstein-Friesian  Local) dairy heifers aged between 4 to 8 months old at the Military Dairy Farm received 2.0 ml imported commercial B. abortus SRB51 strain vaccine subcutaneously in the neck region at day 0 and then booster dose at 60 days after first vaccination with similar dose and route during the period from June to October 2020. Each of the collected serum samples of 20 heifers at day 0, 7, 14, 21, 28, 60, 90, 120 and 150 was tested to detect the antibody status by using commercial indirect ELISA kit. Results: The humoral immune response (HIR) in terms of antibody levels detected by OD values in the serum of immunized cross-bred dairy heifers by using B. abortus strain RB51 commercial vaccine resulted 0.097  0.0032 (mean  SE) OD value at 0 day (i.e. pre-immunization) and 0.108 ± 0.0032 at 7th day. After that, the OD value started to rise from day 14 (OD value 0.124 ± 0.0032) and reached to a peak level at 60 days (OD value 0.223  0.0032) with the initial vaccination. Booster vaccination inoculated at 60 days resulted peak antibody level in terms of OD value (0.313  0.0032) at the day 90 and then the antibody level started to decline from 120 days (OD value 0.242  0.0032) to 150 days (OD value 0.199 0.0032) in cross-bred dairy heifers. Conclusions: This study suggests that the commercial B. abortus RB51 strain vaccine has induced satisfactory HIR with initial inoculation and significantly higher HIR produced with a booster dose in crossbred heifers by using commercial I-ELISA. The presence of Brucella antibodies have importance on sero-diagnosis whereas the cell mediated immunity (CMI) plays major role in protection against brucellosis which needs further investigation in cross-bred heifers in Bangladesh. Keywords: Brucellosis, SRB51 vaccine, Humoral immune response, I-ELISA, Cross-bred heifers, Bangladesh

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Section: Discussionmentioning

confidence: 70%

Humoral Immune Response in Cross-Bred Heifers Immunized With Brucella Abortus Strain Rb51 Vaccine in Military Dairy Farm of Bangladesh

Rahman¹,

Rahman²,

Rahman³

et al. 2020

JVMOHR

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“…39 The antibody titer has been reported to be significantly increased in the vaccinated calves after one month and the titer declined but remained positive up to six months and then negative throughout the 12 months study periods. 23 The antibodies titer began and reached the maximum as early as first week of RB51 vaccination in buffalo calves, remain steady till two weeks post vaccination (WPV), fluctuating till the 6 th WPV, then dropped sharply when it disappeared at 11 WPV till the end of the experiment. 40 This is significantly different from the results of RB51 vaccination in cattle in which an IR that began with an increase at day 6 post-vaccination, the antibody level remained constant for two months, then progressively decreased.…”

Section: Discussionmentioning

confidence: 98%

“…18 The immune responses of live attenuated B. abortus S19 vaccine in cattle and buffaloes have been evaluated in India. 23,24 Comparison of HIR between heat-inactivated B. abortus biovar 3 and strain RB51 in cattle have been studied in Bangladesh. 25 This paper describes the comparison of HIR of commercial B. abortus strain RB51 vaccine between cattle and buffaloes in Bangladesh.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Mohamud¹,

Mohamud²,

Rahman³

et al. 2020

JVMOHR

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Background: The effective control and eradication of brucellosis can be achieved by rapid and accurate diagnosis and effective vaccination but both have limitations. Therefore, brucellosis research is currently focused on the improvement of the diagnosis and vaccine induced prophylaxis. Moreover, diagnostic tests and immunization have not been thoroughly studied in buffaloes and even not compared with cattle. Therefore, the comparative evaluation of the immunological responses of Brucella vaccinated cattle and buffaloes would be required for both the diagnosis and vaccine induced efficacy. Objectives: The main objective of this study was to compare the humoral immune response (HIR) between cattle and buffalo cows immunized with B. abortus RB51 vaccine by using indirect ELISA Materials and Methods: Each of the three randomly selected B. abortus sero-negative native cows and three buffaloes received 2.0 ml imported commercial B. abortus SRB51 vaccine subcutaneously in the neck region at day 0 and then booster dose at 60 days after first vaccination with similar dose and route. Each of the collected serum samples of both the cattle and buffaloes was tested to detect the antibody status by using commercial indirect ELISA kit. Results: The results showed that the OD value of the serum of cows and buffalos before inoculation of RB51 B. abortus vaccine was 0.088 ± 0.009 and 0.096  0.011 at 0 week and 0.124 ± 0.018 and 0.111  0.010 at 1st week, near about the negative control OD value (0.106). After that, the OD value started to rise from the 2nd week (OD value (0.144 ± 0.023 and 0.1333  0.007) and reached to a peak level at 90 days (OD value 0.376  0.0080 and 0.316  0.219) and then started to decline from 120 days (OD value 0.2963  0.0416 and 0.2863  0.070) to 180 days (OD value 0.1943 0.073 and 0.176 0.172) in cows and buffalos respectively. Conclusion: This study suggests that the RB51 vaccination has induced satisfactory HIR with initial inoculation but significantly higher immune responses with booster immunization which enhancing immunity against both in the cattle and buffaloes. The CMI plays major role in protection against brucellosis needs further investigation in both cattle and buffaloes in Bangladesh. Keywords: Brucellosis, SRB51 vaccine, Humoral immune response (HIR), I-ELISA, Cattle and Buffaloes

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Characterization of humoral and cellular immune responses elicited by reduced doses of Brucella abortus S19 (calfhood) vaccine in cattle calves of India

Shome

Kilari²,

Sahare³

et al. 2023

Research in Veterinary Science

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Immune response of cattle to vaccination with Brucella abortus strain 19 in India

Cited by 3 publications

References 3 publications

Humoral Immune Response in Cross-Bred Heifers Immunized With Brucella Abortus Strain Rb51 Vaccine in Military Dairy Farm of Bangladesh

Humoral Immune Response in Cross-Bred Heifers Immunized With Brucella Abortus Strain Rb51 Vaccine in Military Dairy Farm of Bangladesh

Comparison of Humoral Immune Responses Between Cattle and Buffaloes Immunized With Commercial Brucella Abortus Strain Rb51 Vaccine in Bangladesh

Characterization of humoral and cellular immune responses elicited by reduced doses of Brucella abortus S19 (calfhood) vaccine in cattle calves of India

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