Immunization of mice with a Mycobacterium tuberculosis genomic expression library results in lower bacterial load in lungs after challenge with BCG

Hernández, Yamilé López; Yero, Daniel; Rodríguez, Sergio Sifontes; Bourzac, Juan Francisco Infante; Sarmiento, María E.; Arzuaga, Nesty Olivares; Maceo, Enrique Casado; Díaz, Daiyana; Dı́az, Raúl; Acosta, Armando

doi:10.1016/j.tube.2006.01.004

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…injection using a high dose of bacilli ( Hernández-Pando et al, 1996 ) is one of the most employed models. This model has greatly contributed to elucidate the role of antibodies in the protection against mycobacterial infections ( López et al, 2009 , 2010 ; Alvarez et al, 2013 ), and to the screening and validation of new vaccine candidates ( Castillo-Rodal et al, 2006 ; López et al, 2006 ). Alternatively, C57BL mice have been infected i.n.…”

Section: From Classic To Alternative Models In Studying Relevant Bactmentioning

confidence: 99%

Animals devoid of pulmonary system as infection models in the study of lung bacterial pathogens

Hernández¹,

Yero

Pinos-Rodríguez

et al. 2015

Front. Microbiol.

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Biological disease models can be difficult and costly to develop and use on a routine basis. Particularly, in vivo lung infection models performed to study lung pathologies use to be laborious, demand a great time and commonly are associated with ethical issues. When infections in experimental animals are used, they need to be refined, defined, and validated for their intended purpose. Therefore, alternative and easy to handle models of experimental infections are still needed to test the virulence of bacterial lung pathogens. Because non-mammalian models have less ethical and cost constraints as a subjects for experimentation, in some cases would be appropriated to include these models as valuable tools to explore host–pathogen interactions. Numerous scientific data have been argued to the more extensive use of several kinds of alternative models, such as, the vertebrate zebrafish (Danio rerio), and non-vertebrate insects and nematodes (e.g., Caenorhabditis elegans) in the study of diverse infectious agents that affect humans. Here, we review the use of these vertebrate and non-vertebrate models in the study of bacterial agents, which are considered the principal causes of lung injury. Curiously none of these animals have a respiratory system as in air-breathing vertebrates, where respiration takes place in lungs. Despite this fact, with the present review we sought to provide elements in favor of the use of these alternative animal models of infection to reveal the molecular signatures of host–pathogen interactions.

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Section: From Classic To Alternative Models In Studying Relevant Bactmentioning

confidence: 99%

Animals devoid of pulmonary system as infection models in the study of lung bacterial pathogens

Hernández¹,

Yero

Pinos-Rodríguez

et al. 2015

Front. Microbiol.

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show abstract

“…The animals in each of the above groups were immunized three times at 2-week intervals. In the positive control group, mice were inoculated subcutaneously with 1 × 10 6 colony-forming units (CFUs) of BCG at the time of initial vaccination [42].…”

Section: Immunization Of Micementioning

confidence: 99%

“…The left lungs extracted from the mice were homogenized in 0.05% PBST, and serially diluted 10-fold. Following this, 100 l of the diluted samples (10 -3 , 10 -4 , and 10 -5 ) were plated on modified Lowenstein-Jensen medium [42], and the plates were incubated at 37 • C for 4-6 weeks; the number of CFU was then counted [30,47].…”

Section: Challenge and Bacterial Load In Lung Tissuementioning

confidence: 99%

A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice

et al. 2008

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Construction of Genomic Expression Library Vaccines Against Avian Pasteurella Multocida and Detection of the Immune Efficacy

Gong¹,

Qin²,

Cheng³

2013

Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

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Immunization of mice with a Mycobacterium tuberculosis genomic expression library results in lower bacterial load in lungs after challenge with BCG

Cited by 4 publications

References 36 publications

Animals devoid of pulmonary system as infection models in the study of lung bacterial pathogens

Animals devoid of pulmonary system as infection models in the study of lung bacterial pathogens

A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice

Construction of Genomic Expression Library Vaccines Against Avian Pasteurella Multocida and Detection of the Immune Efficacy

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