Immuno- and nucleic acid-based current technique for Salmonella detection in food

Lin, Long-Zhen; Zheng, Qianwang; Lin, Jizhen; Yuk, Hyun‐Gyun; Guo, Li‐Qiong

doi:10.1007/s00217-019-03423-9

Cited by 29 publications

(25 citation statements)

References 178 publications

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“…This low occurrence of Salmonella in raw poultry products agrees with data from other industrialized countries with pathogen reduction programs [ 2 , 9 ]. The presence of Salmonella was confirmed in the same samples by the standard cultural method (ISO 6579) and the PCR assay in total DNA obtained from the selective enrichments, showing that molecular detection is a good alternative to laborious and time-consuming conventional approaches [ 35 ]. Moreover, molecular-based assays (such as PCR and real-time-PCR) are powerful tools to be used by poultry companies in combination with cultural-based methods as they overcome the lack in the detection of low counts, viable non-cultivable cells or atypical biochemical Salmonella profiles [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…The presence of Salmonella was confirmed in the same samples by the standard cultural method (ISO 6579) and the PCR assay in total DNA obtained from the selective enrichments, showing that molecular detection is a good alternative to laborious and time-consuming conventional approaches [ 35 ]. Moreover, molecular-based assays (such as PCR and real-time-PCR) are powerful tools to be used by poultry companies in combination with cultural-based methods as they overcome the lack in the detection of low counts, viable non-cultivable cells or atypical biochemical Salmonella profiles [ 35 ]. Nevertheless, molecular methods would still benefit from further improvements in terms of sensitivity at the pre-enrichment step [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

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Atypical Non-H2S-Producing Monophasic Salmonella Typhimurium ST3478 Strains from Chicken Meat at Processing Stage Are Adapted to Diverse Stresses

et al. 2020

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Poultry products are still an important cause of Salmonella infections worldwide, with an increasingly reported expansion of less-frequent serotypes or atypical strains that are frequently multidrug-resistant. Nevertheless, the ability of Salmonella to survive antimicrobials promoted in the context of antibiotic reducing/replacing and farming rethinking (e.g., organic acids and copper in feed/biocides) has been scarcely explored. We investigated Salmonella occurrence (conventional and molecular assays) among chicken meat at the processing stage (n = 53 batches/29 farms) and characterized their tolerance to diverse stress factors (antibiotics, copper, acid pH, and peracetic acid). Whole-genome sequencing was used to assess adaptive features and to perform comparative analysis. We found a low Salmonella occurrence (4%) and identified S. Enteritidis/ST11 plus atypical non-H2S-producing S. 1,4,[5],12:i:-/ST3478. The ST3478 presented the ability to grow under diverse stresses (antibiotics, copper, and acid-pH). Comparative genomics among ST3478 isolates showed similar antibiotic/metal resistance gene repertoires and identical nonsense phsA thiosulfate reductase mutations (related to H2S-negative phenotype), besides their close phylogenetic relationship by cgMLST and SNPs. This study alerts for the ongoing national and international spread of an emerging monophasic Salmonella Typhimurium clonal lineage with an enlarged ability to survive to antimicrobials/biocides commonly used in poultry production, being unnoticed by conventional Salmonella detection approaches due to an atypical non-H2S-producing phenotype.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Atypical Non-H2S-Producing Monophasic Salmonella Typhimurium ST3478 Strains from Chicken Meat at Processing Stage Are Adapted to Diverse Stresses

et al. 2020

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“…For example, during the last decade, confirmed cases of Salmonella -caused illnesses have dropped by ca. 40%, due to significantly improved quality of food analysis, with cases reported increased six-fold [ 171 ]. Along with that, due to some inherent limitations regarding the bacterial assays’ time, sensitivity and often unaffordable for some application cost, some human activity fields still challenge the bacterial sensor market.…”

Section: Future Perspectivesmentioning

confidence: 99%

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Jamal

Shipovskov

Ferapontova

2020

Sensors

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Microbiological safety of the human environment and health needs advanced monitoring tools both for the specific detection of bacteria in complex biological matrices, often in the presence of excessive amounts of other bacterial species, and for bacteria quantification at a single cell level. Here, we discuss the existing electrochemical approaches for bacterial analysis that are based on the biospecific recognition of whole bacterial cells. Perspectives of such assays applications as emergency-use biosensors for quick analysis of trace levels of bacteria by minimally trained personnel are argued.

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“…They are immunological-based assays, such as enzyme-linked immunosorbent assay (ELISA), latex agglutination method, and immunochromatography assay [ 4 , 5 , 6 ]; molecular-based assays, e.g., polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), recombinase polymerase amplification (RPA), DNA microarrays, whole genome-sequencing (WGS) [ 7 , 8 , 9 , 10 , 11 , 12 ]; mass spectrometry-based methods, e.g., matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF MS), liquid chromatography-mass spectrometry (LC-MS) [ 13 , 14 ]; spectroscopy-based methods, e.g., Raman spectroscopy, near-infrared (NIR) spectroscopy, and hyperspectral imaging (HSI) [ 15 , 16 , 17 ], optical phenotyping with light diffraction technology [ 18 ], and electrochemical biosensors [ 19 ]. These methods can identify and discriminate Salmonella down to their serotype level [ 14 , 20 , 21 , 22 , 23 ]. Apart from these detection methods, electrochemical biosensors could potentially be an ideal tool for Salmonella detection, since they are capable of detecting the presence of Salmonella or their cellular components in just a few hours.…”

Section: Introductionmentioning

confidence: 99%

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

et al. 2021

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Large-scale food-borne outbreaks caused by Salmonella are rarely seen nowadays, thanks to the advanced nature of the medical system. However, small, localised outbreaks in certain regions still exist and could possess a huge threat to the public health if eradication measure is not initiated. This review discusses the progress of Salmonella detection approaches covering their basic principles, characteristics, applications, and performances. Conventional Salmonella detection is usually performed using a culture-based method, which is time-consuming, labour intensive, and unsuitable for on-site testing and high-throughput analysis. To date, there are many detection methods with a unique detection system available for Salmonella detection utilising immunological-based techniques, molecular-based techniques, mass spectrometry, spectroscopy, optical phenotyping, and biosensor methods. The electrochemical biosensor has growing interest in Salmonella detection mainly due to its excellent sensitivity, rapidity, and portability. The use of a highly specific bioreceptor, such as aptamers, and the application of nanomaterials are contributing factors to these excellent characteristics. Furthermore, insight on the types of biorecognition elements, the principles of electrochemical transduction elements, and the miniaturisation potential of electrochemical biosensors are discussed.

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Immuno- and nucleic acid-based current technique for Salmonella detection in food

Cited by 29 publications

References 178 publications

Atypical Non-H2S-Producing Monophasic Salmonella Typhimurium ST3478 Strains from Chicken Meat at Processing Stage Are Adapted to Diverse Stresses

Atypical Non-H2S-Producing Monophasic Salmonella Typhimurium ST3478 Strains from Chicken Meat at Processing Stage Are Adapted to Diverse Stresses

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes

Advancement in Salmonella Detection Methods: From Conventional to Electrochemical-Based Sensing Detection

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