Immunochemical analysis and localisation of a cell wall antigen of Streptococcus rattus with a specific monoclonal antibody

Ota, Fusao; Kato, Hirohisa; Hirota, Kaoru; Imai, Shoichi; Ishikawa, Hiroyuki; Yamato, Masayuki; Minato, Yoshihiro

doi:10.1016/s0934-8840(11)80703-6

Cited by 2 publications

(7 citation statements)

References 20 publications

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“…1 Part of reactivity was reported previously [Ota et al, 1995;Kato et al, 1986;Nagamune et al, 1992]. No strains grew at 45 ° C, in the presence of 6.5% NaCl or at pH 9.6.…”

Section: Reactivity Of Mabs With Other Bacterial Species Found In Thementioning

confidence: 60%

“…Hardie and Dr. R.A. Whiley, London University. Other strains were stock cultures from our laboratories [Ota et al, 1995].…”

Section: Reference Strainsmentioning

confidence: 99%

“…Preparation of mAbs mAbs were prepared as described before [Kato et al, 1986;Ota et al, 1995]. In brief, BALB/c mice were immunised intraperitoneally twice with either live or heat-killed cells of strain HS1 (serotype a) or strain FA1 (serotype b), strain Ingbritt (serotype c), OMZ175 (serotype f), MT557 (serotype f), OMZ176 (serotype d), MT615R (serotype d), emulsifi ed with Freund's incomplete adjuvant.…”

Section: Isolation Identifi Cation and Culture Conditionsmentioning

confidence: 99%

“…The hybridoma cells were cultured and injected into pristane-pretreated BALB/c mice. Ascitic fl uids were collected and subjected to salt precipitation with ammonium sulphate or partially purifi ed as described previously [Kato et al, 1986;Ota et al, 1995]. Culture fl uids of a hybridoma were also used with or without dilution.…”

Section: Isolation Identifi Cation and Culture Conditionsmentioning

confidence: 99%

“…Reactivity of mAbs with whole cells of reference strains listed in table 1 was examined by EIA as described before Ota et al, 1995]. Briefl y, reference strains were cultured in BHIB overnight, harvested, washed in PBS by centrifugation and suspended to an optical density of 0.5 at 660 nm in PBS.…”

Section: Eia For Antibody Specifi City and Serological Identifi Catiomentioning

confidence: 99%

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Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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A set of monoclonal antibodies were prepared by the conventional cell fusion of myeloma cells (SP2/0-Ag14) with spleen cells from BALB/c mice immunised with whole cells of a strain of mutans streptococci. Their specificities were examined against 35 reference strains of mutans streptococci, 34 reference strains of other oral streptococci and 8 reference strains of other microorganisms often inhabiting the oral cavity. Specificity was examined by enzyme immunoassay using whole cells. A total of 52 strains, consisting of 19 strains isolated in Japan, 19 strains isolated in Italy and 14 strains isolated in England, were characterised by conventional physiological and biochemical tests and then serotyped by the use of 8 monoclonal antibodies with different specificities. They were also confirmed by guanine-plus-cytosine contents of their nucleic acid and DNA-DNA hybridisation test. The results indicated that all monoclonal antibodies are useful for identification of 8 serotypes of the mutans streptococci responsible for dental caries. They also suggest the existence of more serological varieties among mutans species.

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“…1 Part of reactivity was reported previously [Ota et al, 1995;Kato et al, 1986;Nagamune et al, 1992]. No strains grew at 45 ° C, in the presence of 6.5% NaCl or at pH 9.6.…”

Section: Reactivity Of Mabs With Other Bacterial Species Found In Thementioning

confidence: 60%

“…Hardie and Dr. R.A. Whiley, London University. Other strains were stock cultures from our laboratories [Ota et al, 1995].…”

Section: Reference Strainsmentioning

confidence: 99%

Section: Isolation Identifi Cation and Culture Conditionsmentioning

confidence: 99%

Section: Isolation Identifi Cation and Culture Conditionsmentioning

confidence: 99%

Section: Eia For Antibody Specifi City and Serological Identifi Catiomentioning

confidence: 99%

See 3 more Smart Citations

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Ota

Ota²,

Mahmud³

et al. 2005

Caries Res

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show abstract

Genetic studies on reference strains of mutans streptococci

Ota

Yamato

Hayashi

et al. 2002

Microbiological Research

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Twenty four reference strains (serotype a-h) belonging to the mutans group of streptococci were compared for DNA fragment patterns of rDNA after treatment with Hind III. It was shown that Streptococcus cricetus (serotype a), S. rattus (serotype b), and S. downei (serotype h) reveals comparatively homogeneous patterns while S. mutans (serotype c, e and f) exhibits differences between the different serotypes as well as within single serotypes. S. sobrinus had an intermediary diversity. These data support the previous findings that S. mutans is heterogeneous at the serological, biochemical and genetical level.

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Immunochemical analysis and localisation of a cell wall antigen of Streptococcus rattus with a specific monoclonal antibody

Cited by 2 publications

References 20 publications

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Serological Diversity Demonstrable by a Set of Monoclonal Antibodies to Eight Serotypes of the Mutans Streptococci

Genetic studies on reference strains of mutans streptococci

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