Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

Sotnikov, Dmitriy V.; Barshevskaya, Lyubov V; Zherdev, Anatoly V.; Eskendirova, Saule; Mukanov, Kassym K.; Mukantayev, Kanatbek; Ramankulov, Yerlan; Dzantiev, Boris B.

doi:10.3390/app10030738

Cited by 11 publications

(11 citation statements)

References 38 publications

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“…The serum samples of cows were collected in the course of previous investigations of the authors [23][24][25]27]. Blood serum were obtained from the "Russian Black Pied" and 'Red Steppe' cattle breeds aged from 1 to 4 years and weighing from 200 to 450 kg, "Kazakh Whiteheaded" cattle breed mostly at the age of 2 years and weighing from 200 to 550 kg.…”

Section: Serum Panelsmentioning

confidence: 99%

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Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

et al. 2019

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An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.

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Section: Serum Panelsmentioning

confidence: 99%

“…Immunochromatographic tests for serodiagnosis of brucellosis in animal species have been described [22][23][24][25]. Kim et al have developed an immunochromatographic system for the detection of specific antibodies against the bovine leukemia virus [26].…”

Section: Introductionmentioning

confidence: 99%

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

et al. 2019

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“…Previously, studies indicated that ferrite NPs are used as a diagnostic tool for high saturation magnetization, tissue relaxivity, clarity, and exact localization of these particles in the target tissue [9][10][11][12]. It is reported that iron oxide being ferrite in nature is extensively used in various fields of nanotechnology [13][14][15]. Reports indicate that among different ferrites, nickel and cobalt ferrites are also used to increase the high magnetic moment of ferrites in biomedical applications [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Hematobiochemical, Oxidative Stress, and Histopathological Mediated Toxicity Induced by Nickel Ferrite (NiFe2O4) Nanoparticles in Rabbits

Khan

Buzdar

Hussain

et al. 2022

Oxidative Medicine and Cellular Longevity

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From the past few decades, attention towards the biological evaluation of nanoparticles (NPs) has increased due to the persistent and extensive application of NPs in various fields, including biomedical science, modern industry, magnetic resonance imaging, and the construction of sensors. Therefore, in the current study, magnetic nickel ferrite (NiFe2O4) nanoparticles (NFNPs) were synthesized and evaluated for their possible adverse effects in rabbits. The crystallinity of the synthesized NFNPs was confirmed using X-ray diffraction (XRD) technique. The saturation magnetization (46.7 emug-1) was measured using vibrating sample magnetometer (VSM) and 0.35-tesla magnetron by magnetic resonance imaging (MRI). The adverse effects of NFNPs on blood biochemistry and histoarchitecture of the liver, kidneys, spleen, brain, and heart of the rabbits were determined. A total of sixteen adult rabbits, healthy and free from any apparent infection, were blindly placed in two groups. The rabbits in group A served as control, while the rabbits in group B received a single dose (via ear vein) of NFNPs for ten days. The blood and visceral tissues were collected from each rabbit at days 5 and 10 of posttreatment. The results on blood and serum biochemistry profile indicated significant variation in hematological and serum biomarkers in NFNP-treated rabbits. The results showed an increased quantity of oxidative stress and depletion of antioxidant enzymes in treated rabbits. Various serum biochemical tests exhibited significantly higher concentrations of different liver function tests, kidney function tests, and cardiac biomarkers. Histopathologically, the liver showed congestion, edema, atrophy, and degeneration of hepatocytes. The kidneys exhibited hemorrhages, atrophy of renal tubule, degeneration, and necrosis of renal tubules, whereas coagulative necrosis, neutrophilic infiltration, and severe myocarditis were seen in different sections of the heart. The brain of the treated rabbits revealed necrosis of neurons, neuron atrophy, and microgliosis. In conclusion, the current study results indicated that the highest concentration of NPs induced adverse effects on multiple tissues of the rabbits.

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“…The disease is an ancient disease that has been known under various names, including gastric remittent fever, Mediterranean fever, undulant fever, Malta fever, Bang's disease, and Rock fever of Gibraltar. Humans are accidental hosts; but brucellosis remains a major public health issue across the globe and is the most common zoonosis (Diab et al 2018;Sotnikov et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Serological and Molecular Epidemiological Study on Ruminant Brucellosis in Matrouh Province, Egypt

2022

Int J Vet Sci

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A cross-sectional study was conducted in Matrouh governorate, Egypt from July 2019 until March 2020. A total of 500 farm animals including cattle (n=50), sheep (n=180), and goats (n=270) were examined serologically for brucellosis using Rose Bengal plate (RBPT) test, buffered acidified plate antigen test (BAPAT), and Complement fixation (CFT) test. Multiplex PCR was carried out as a further confirmatory test for the detection of specific genes of Brucella. Statistical analysis was applied using Chi 2 and SPSS 16.0 statistics. The overall prevalence of brucellosis in examined farm animals by RBPT, BAPAT, CFT, and PCR were 11.8, 10.2, 9.4, and 8.6%, respectively with a statistically nonsignificant association between recorded rates. Based on the results of RBPT, it was found that the highest prevalence was recorded in goats (14.81%), followed by sheep (8.89%) and lastly cattle (6%). In addition, it was observed that females were more affected than males and older sheep were more susceptible to infection while younger goats and cattle were more susceptible than adult. Conclusively, brucellosis is alarming in Matrouh Province particularly within goat flocks. There was an urgent need for conducting a proper brucellosis control program and a greater focus should be placed on improving the animal health delivery system in large provinces that share borders with other countries. The public health importance of brucellosis was discussed, and it is always urgent to put in place an integrated strategy to fight against humans and animals' infectious diseases.

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Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots

Cited by 11 publications

References 38 publications

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia

Hematobiochemical, Oxidative Stress, and Histopathological Mediated Toxicity Induced by Nickel Ferrite (NiFe2O4) Nanoparticles in Rabbits

Serological and Molecular Epidemiological Study on Ruminant Brucellosis in Matrouh Province, Egypt

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