Immunocytochemical and ultrastructural characterization of endocrine cells and nerves in the intestine of Rana temporaria

Valverde, Emilio; Rada, O; Burrell, Marı́a A.; Rovira, Jordi; Sesma, P.

doi:10.1016/0040-8166(93)90004-5

Cited by 16 publications

(5 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results on somatostatin-immunoreactive cells showing their distribution throughout all alimentary tracts except the large intestine were consistent with the previous results from Xenopus laevis by Hacker et al (1983), R. temporaria by Valverde et al (1993), R. esculenta by Trandaburu and Nurnberger (1995), and Bufo regularis by . Buchan (1986) also identified somatostatin-immunoreactive cells in the stomach and small intestine of B. bombina, Alytes obstetricans, and Atelopus oxyrhynchus and in the stomach and whole intestinal tracts of R. temporaria, Bufo bufo, and Acris crepitans, but indicated that they were observed only in the stomach and small intestine of R. esculenta and H. arborea, respectively.…”

Section: Discussionsupporting

confidence: 95%

“…The distribution of PP-immunoreactive cells, called pancreatic endocrine cells varied by species as known in previous research (El-Salhy et aJ., 1981;Valverde et a!., 1993). Buchan (1986), however, presented different results from two anurans and eight anomuran species, which showed no PP reactivity.…”

Section: Korean J Bidmentioning

confidence: 83%

“…To date, most research on amphibian enteroendocrine cells have elucidate the function of the ultrastructure of these cells. Several immunohistochemical studies also have been carried out for these cells, but limited to specific organs which provide researchers with fragmentary information (Rajjo et al, 1988;Valverde et al, 1993;Trandaburu and Nllrnberger, 1995;Bodegas et al, 1997). The present study aims to characterize these cells on the whole alimentary tracts of six frog species inhabiting Korea, some of which have never been studied for this purpose.…”

mentioning

confidence: 97%

See 2 more Smart Citations

Immunohistochemical localization of endocrine cells in the alimentary tracts of six frog species

Choi¹,

Moon

Lee

1999

Korean Journal of Biological Sciences

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 95%

Section: Korean J Bidmentioning

confidence: 83%

mentioning

confidence: 97%

See 1 more Smart Citation

Immunohistochemical localization of endocrine cells in the alimentary tracts of six frog species

Choi¹,

Moon

Lee

1999

Korean Journal of Biological Sciences

View full text Add to dashboard Cite

“…Our results show that NT immunoreactivity occurs in mucosal endocrine cells, as described previously in the urodele N. maculosus (Holmgren et al 1985) and the anurans R. temporaria, X. laevis (Reinecke et al 1980;Valverde et al 1993), andB. regularis (El-Salhy et al 1981).…”

Section: Discussionsupporting

confidence: 71%

“…e Numerous gastrin/cholecystokinin (GAS/CCK)-IR enteroendocrine cells were found in the upper small intestine. ×400 tebrates (Reinecke et al 1981(Reinecke et al , 1997Valverde et al 1993). This may indicate that VIP has a general importance as relaxant in the gastrointestinal tract (Grider and Makhlouf 1988;Gonda et al 1989) throughout phylogeny.…”

Section: Discussionmentioning

confidence: 93%

Neurohormonal peptides, serotonin, and nitric oxide synthase in the enteric nervous system and endocrine cells of the gastrointestinal tract of neotenic and thyroid hormone-treated axolotls ( Ambystoma mexicanum )

Maake¹,

Kloas

Szendefi³

et al. 1999

Cell and Tissue Research

View full text Add to dashboard Cite

Immunoreactivity against vasoactive intestinal polypeptide (VIP), neurotensin (NT), substance P (SP), calcitonin gene-related peptide (CGRP), gastrin/cholecystokinin (GAS/CCK), somatostatin (SOM), serotonin (SER), and nitric oxide synthase (NOS) was investigated in the gastrointestinal tract of the urodele Ambystoma mexicanum, the axolotl, by the use of immunohistochemical techniques. The study also compares the distribution patterns and frequencies of the neurohormones, and NOS in neotenic and thyroxine-treated (metamorphosed) individuals. GAS/CCK, SP, NT, SOM, and SER immunoreactivities occurred in endocrine mucosal cells and VIP, SP, CGRP, NTSER, SER, and NOS immunoreactivities in the enteric nervous system. The GAS/CCK-immunoreactive (-IR) cells were restricted to the upper small intestine. NT-IR and SP-IR endocrine cells were found in the entire gastrointestinal tract and were most prominent in the distal large intestine. The density of the SOM-IR cells decreased from the stomach toward the large intestine. SER-IR endocrine cells were found throughout the gastrointestinal tract, with particularly high densities in the stomach and distal large intestine. The VIP-IR enteric nerve fibers were the most prominent ones, present in all layers of the entire gastrointestinal tract, and supplied the smooth muscle and the vasculature. The SER-IR fibers exhibited similar distribution patterns but were less numerous. Very few NT-IR but many SP-IR fibers were found in the muscle and submucosal layers. The NT-IR fibers mainly supplied blood vessels, while the SP-IR fibers were also in contact with the smooth muscle. In the muscle and submucosal layers, CGRP-IR fibers were associated to the vasculature; CGRP immunoreactivity occurred also in a minority of SP-IR fibers. NOS-IR nerve fibers were in contact with submucosal arteries but were the least frequent. After metamorphosis provoked by exogenous thyroxine, the number of SOM-IR endocrine cells in the stomach mucosa was increased as well as the density of VIP-IR, SER-IR, and SP-IR nerve fibers in the gastrointestinal tract. It is proposed that the observed increases may reflect refinements of the neurohormonal system after metamorphosis.

show abstract

Histology and lectin-binding patterns in the digestive tract of the carnivorous larvae of the Anuran,Ceratophrys ornata

Fry

Kaltenbach

1999

J. Morphol.

View full text Add to dashboard Cite

Immunocytochemical and ultrastructural characterization of endocrine cells and nerves in the intestine of Rana temporaria

Cited by 16 publications

References 27 publications

Immunohistochemical localization of endocrine cells in the alimentary tracts of six frog species

Immunohistochemical localization of endocrine cells in the alimentary tracts of six frog species

Neurohormonal peptides, serotonin, and nitric oxide synthase in the enteric nervous system and endocrine cells of the gastrointestinal tract of neotenic and thyroid hormone-treated axolotls ( Ambystoma mexicanum )

Histology and lectin-binding patterns in the digestive tract of the carnivorous larvae of the Anuran,Ceratophrys ornata

Contact Info

Product

Resources

About