Immunocytochemistry of Male Reproductive Organs

Abstract

Immunological techniques have enabled us to see that mammalian sperm undergo complex surface changes during maturation in the male reproductive tract. Binding affinity and sperm surface binding domains have been demonstrated using immunocytochemical technique. Recent studies using monoclonal antibodies suggest that these highly specific probes are useful for detecting changes in the sperm surface during epididymal transit and in defining the role of these complex changes in sperm maturation and the process of fertilization. Studies involving immunological mapping of the sperm surface, in parallel with immunohistological and functional inhibition test, have provided important information concerning the role of individual sperm antigens in fertility. A better understanding of local antibody production and cell-mediated immune responses in the male reproductive tract has also led to the understanding of immunological infertility. Sperm membrane is comprised of multiple domains each of which is sharply demarcated, with a unique composition and physiological role.

GPI-anchored complement regulatory proteins in seminal plasma. An analysis of their physical condition and the mechanisms of their binding to exogenous cells.

“…By Western blotting using mAb anti-CD59 (Fig. 8 B), a 20-kD band was detected in fractions of the first peak, whereas no anti-CD59 material was detected in fractions [26][27][28][29][30].…”

“…Sperm are at risk of C attack within the female reproductive tract (28) and C inhibitors may be of importance for fertility. Further, sperm acquire new proteins during passage through the epididymis (29). It will be of interest to determine whether these acquired proteins are GPI anchored and whether a micellar intermediate is involved in transfer.…”