Steel dominates the global metal production accounting for 5 % of increase in Earth's atmospheric carbon dioxide (CO 2 ). Today, India is the 4th largest producer of crude steel in the world. The sector contributes around 3 % to the country's gross domestic product (GDP) but adds 6.2 % to the national greenhouse gas (GHG) load. It accounts for 28.4% of the entire industry sector emissions, which are 23.9% of the country's total emissions. Being a developing country, India is not obliged to cut its emissions under the Kyoto Protocol to the United Nations Framework Convention on Climate Change (FCCC), but gave voluntary commitment to reduce the emission intensity of its GDP by 20-25 % from the 2005 level by 2020. This paper attempts to find out if the Indian steel sector can help the country in fulfilling this commitment. The sector reduced its CO 2 emissions per ton of steel produced by 58% from 1994 to 2007. The study generates six scenarios for future projections which show that the sector can reduce its emission intensity by 12.5 % to 63 %. But going by the conservative estimates, the sector can reduce emission intensity by 30 % to 53 %. However, actual emissions will go up significantly in every case.

Two isoenzymes of N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) (Hex A and Hex B) from human seminal plasma were purified to homogeneity with specific activities of 26 and 60 units/mg of protein respectively. N-Acetyl-beta-D-glucosaminidase activity was inseparable from N-acetyl-beta-D-galactosaminidase activity in both Hex A and Hex B by various conventional chromatographic procedures. Although Km values of N-acetyl-beta-glucosaminidase activity of Hex A and Hex B were similar (1.33 mM), those of N-acetyl-beta-galactosaminidase activity were 0.14 mM for Hex A and 0.40 mM for Hex B. However, pH optima and temperature optima were identical for N-acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase activities of both isoenzymes; Hex A was far more heat-sensitive than Hex B. Thiol-reactive compounds such as silver salts, mercuric salts, p-chloromercuribenzoate and thimerosal strongly inhibited the N-acetyl-beta-glucosaminidase activities of both isoenzymes. GSH protected the enzyme activities from inactivation caused by these reagents, confirming the presence of thiol groups at the active centres. Inhibitions of N-acetyl-beta-glucosaminidase activities of both isoenzymes by metal salts and organic anions were comparable; acetate and arsenite were effective inhibitors for both isoenzymes. In contrast, inhibitions of N-acetyl-beta-glucosaminidase activities of the two isoenzymes by iodoacetic acid, iodoacetamide and ethylmaleimide were not comparable; Hex B was more susceptible to inhibition by these agents at 20 mM concentration. The N-acetyl-beta-glucosaminidase activities of both isoenzymes are strongly inhibited, in decreasing order, by N-acetyl-galactosamine, mannosamine, disaccharic acid lactone, N-acetylglucosamine and gluconolactone. The Ki values of the N-acetyl-beta-glucosaminidase and N-acetyl-beta-galactosaminidase activities for N-acetylhexosamines and results from mixed-substrate kinetics indicated that the activities for the two substrates are located at different sites in Hex A and at the same site in Hex B. The Mr values of Hex A and Hex B were determined to be 195,000 and 210,000 respectively by gel filtration through Sephadex G-200. SDS/polyacrylamide-gel electrophoresis revealed that Hex A and Hex B are each composed of four subunits corresponding to Mr about 50,000 each. No further polypeptide chain was obtained after reduction and alkylation of Hex A and Hex B with 10 mM-dithiothreitol and 10 mM-iodoacetamide.

Immunocytochemical localization of hexosaminidase activity in human males revealed that the enzyme activity is localized mainly in the Sertoli cells and interstitial tissue of the testis and in the columnar cells of the epididymis. In seminal vesicles, activity was observed around the glandular epithelium in the form of fine granules.

Purpose This study aims to examine the impact of signaling through social media (SM) on funding achieved by start-ups. Design/methodology/approach This study follows a causal research design and is based on unique data set compiled from Crunchbase-Pro and Twitter. The sample size is 1,672 Indian start-ups. Heckman’s model and ordinary least squares regression is used to test the hypothesis. Findings Devising a thoughtful SM strategy, should be an integral part of the overall strategy of the start-ups looking out for funds. LinkedIn presence is in itself a positive signal. Active usage of Twitter and feedback from other Twitter users has a positive impact on funds raised by the start-up. Posting retweets and repetitive usage of URLs and media is not a predictor of funds raised by the start-up. Practical implications An early-stage strategy on SM adoption, especially Twitter can play an important role in attracting interest and attention of stakeholders. To capitalize SM, entrepreneurs should maintain an active SM account of the start-up. Originality/value India has emerged as one of the start-up hubs of the world. However, there is a dearth of literature on SM usage by start-ups in India. To the best of the authors’ knowledge, this study is first of its kind and establishes the results empirically based on more than 100k tweets for a large pool of Indian start-ups.

Immunological techniques have enabled us to see that mammalian sperm undergo complex surface changes during maturation in the male reproductive tract. Binding affinity and sperm surface binding domains have been demonstrated using immunocytochemical technique. Recent studies using monoclonal antibodies suggest that these highly specific probes are useful for detecting changes in the sperm surface during epididymal transit and in defining the role of these complex changes in sperm maturation and the process of fertilization. Studies involving immunological mapping of the sperm surface, in parallel with immunohistological and functional inhibition test, have provided important information concerning the role of individual sperm antigens in fertility. A better understanding of local antibody production and cell-mediated immune responses in the male reproductive tract has also led to the understanding of immunological infertility. Sperm membrane is comprised of multiple domains each of which is sharply demarcated, with a unique composition and physiological role.