Immunocytochemistry of Odorant-Binding Proteins

Steinbrecht, Rudolf Alexander; Laue, Michael; Zhang, S.-G.; Ziegelberger, Gunde

doi:10.1007/978-4-431-68355-1_327

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…PBPs are mostly located in male antenna, in the Archives of Insect Biochemistry and Physiology sensilla specifically responding to pheromones, thus supporting their role in pheromone detection (Steinbrecht et al, 1992(Steinbrecht et al, , 1994(Steinbrecht et al, , 1995Maida et al, 1993). However, Callahan et al (2000) recently reported that in three lepidopteran species the expressed quantity of PBP was similar in the two sexes.…”

Section: Introductionmentioning

confidence: 95%

“…However, Lartigue et al (2003) reported the that crystal structures are different between PBPs of Leucophaea maderae and B. mori, which suggests that different binding and release mechanisms may be adapted to the hydrophilicity or hydrophobicity of the pheromonal ligand. In addition, immunocytochemistry offers the possibility of localizing these molecules in sections of moth antennae with high selectivity and spatial resolution (Steinbrecht et al, 1992(Steinbrecht et al, , 1994(Steinbrecht et al, , 1995Maida et al, 1993;Wang et al, 2003). Thus, it is possible to clarify the question concerning the correlation between the presence or absence of a certain binding protein and the specific olfactory function of a given sensillum.…”

Section: Introductionmentioning

confidence: 98%

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Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hübner)

Wang

Guo

2004

Arch Insect Biochem Physiol

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A cDNA clone coding for pheromone binding protein was isolated from the antennae of Helicoverpa armigera by RT-PCR and (5'/3')-RACE technique. The full-length of H. armigera pheromone binding protein (HarmPBP) was 952 bp, possessing 162 amino acid residues including a signal peptide of 20 amino acids. Its predicted molecular weight and isoelectric point were 18.26 kDa and 5.23, respectively. This deduced amino acid sequence shared some common structural features with odorant-binding proteins from several moth species, including the six conserved cysteine motif, a typical characteristic of insect's odorant-binding proteins. Northern blot showed that HarmPBP is specifically expressed in the antennae of Helicoverpa armigera and more abundantly expressed in male than female. During the antennal development, HarmPBP is first expressed about 4 days prior to adult eclosion and rises to a plateau 2 days prior to adult eclosion. In order to obtain sufficient PBP for further determining its biochemical and physiological properties, a bacterical expression vector of PBP was constructed and successfully expressed in Escherichia coli. The recombinant PBP was shown to cross-react with an anti-PBP antiserum from Antheraea polyphemus. Polyclonal antibodies against HarmPBP were used to mark the distribution of the protein in olfactory sensilla. Very strong labeling was observed in the sensillum lymph of the hair lumen and of the sensillum-lymph cavity. In the male, HarmPBP is expressed in sensilla trichodea and not in sensilla basiconica, while in the female, it is expressed both in sensilla basiconica and sensilla trichodea.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hübner)

Wang

Guo

2004

Arch Insect Biochem Physiol

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Immunolocalization of pheromone-binding protein and general odorant-binding protein in olfactory sensilla of the silk moths Antheraea and Bombyx

Steinbrecht¹,

Laue²,

Ziegelberger³

1995

Cell Tissue Res

198

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Olfaction in Lepidoptera

1995

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Abstract. Odours play a very important role in the life of insects belonging to the order Lepidoptera. In the present paper, a review is given of the current knowledge of morphology, development and function of the olfactory system in larval and adult moths and butterflies. Research regarding both the antennal and accessory olfactory pathways, as well as both the pheromone and the host odour detecting systems, is reviewed.

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Immunocytochemistry of Odorant-Binding Proteins

Cited by 5 publications

References 17 publications

Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hübner)

Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hübner)

Immunolocalization of pheromone-binding protein and general odorant-binding protein in olfactory sensilla of the silk moths Antheraea and Bombyx

Olfaction in Lepidoptera

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