Immunofluorescence–based biosensor for the determination of dengue virus NS1 in clinical samples

Darwish, Nadiya Taha; Sekaran, Shamala Devi; Alias, Yatimah; Khor, Sook Mei

doi:10.1016/j.jpba.2017.11.064

Cited by 35 publications

(23 citation statements)

References 48 publications

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“…As shown in Fig C in S1 File, the limit of detection (LOD) was calculated by the following equation LOD = 3 (σ/ S ) [43], where, σ is the standard deviation and S is the sensitivity and LOD of our sensor system was found to be 1.49 μg/mL which is slightly lower or comparable to that of the previously reported biosensor [1, 44]. However, it is noteworthy that LOD value in our sensor is close to the level of NS1 in primary (0.04 to 2 μg/mL) and secondary DENV infection (0.01 to 2 μg/mL) in patients [8, 9] suggesting quite acceptable for clinical testing. Importantly, a strong linearity with good correlation (regression coefficient R 2 = 0.91) between Δ I % and NS1 concentration was seen in ranging from 0.003 to 1.25 μg/mL (inset in Fig 4C).…”

Section: Resultssupporting

confidence: 69%

“…Some reports suggest that the glycoprotein, nonstructural 1 (NS1), is a useful biomarker of infection because of its release and high level accumulation at concentrations up to 50 μg/mL in dengue patients [6, 7]. In the clinic, serum NS1 levels range from 0.04 to 2 μg/mL in patients with primary DENV infection and from 0.01 to 2 μg/mL in those with secondary infection [8, 9]. Therefore, a number of commercial rapid kits for DENV NS1 detection have been developed and characterized [3, 8, 10–13].…”

Section: Introductionmentioning

confidence: 99%

“…In the clinic, serum NS1 levels range from 0.04 to 2 μg/mL in patients with primary DENV infection and from 0.01 to 2 μg/mL in those with secondary infection [8, 9]. Therefore, a number of commercial rapid kits for DENV NS1 detection have been developed and characterized [3, 8, 10–13]. Combining NS1 with IgM and IgG can improve the sensitivity and specificity of DENV detection, compared to NS1 alone [8, 14, 15].…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a number of commercial rapid kits for DENV NS1 detection have been developed and characterized [3, 8, 10–13]. Combining NS1 with IgM and IgG can improve the sensitivity and specificity of DENV detection, compared to NS1 alone [8, 14, 15]. However, these methods rely on polyclonal or monoclonal antibodies and involve multistep sample preparation [14].…”

Section: Introductionmentioning

confidence: 99%

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Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

et al. 2019

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Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Potential affinity peptides specific for NS1 were chosen according to the dynamic current decrease in SWV experiments. Using circular dichroism, the molar ellipticity of peptides (DGV BP1–BP5) was determined, indicating that they had a mostly similar in random coil structure, not totally identical. Using SWV, DGV BP1 was selected as a promising recognition peptide and limit of detection for NS1 was found to be 1.49 μg/mL by the 3-sigma rule. DGV BP1 showed good specificity and stability for NS1, with low signal interference. The validation of the sensor to detect NS1 proteins was confirmed with four dengue virus culture broth (from serotype 1 to 4) as proof-of-concept. The detection performance of our sensor incorporating DGV BP1 peptides showed a statistically significant difference. These results indicate that this strategy can potentially be used to detect the dengue virus antigen, NS1, and to diagnosis dengue fever within a miniaturized portable device in point-of-care testing.

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Section: Resultssupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

et al. 2019

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“…[ 31–33 ] Two key aspects that can be modified in ELISA‐IFA to further improve its performance and functionality are the proper immobilization of capture biomolecules and the use of optimal surface blocking agents to reduce the background noise often associated with fluorescence‐based assays. [ 45–47 ] For immobilization of biorecognition elements, contact printing and noncontact printing strategies have been widely used to generate microarrays of biorecognition elements (e.g., antibodies) using desired chemical linkers (e.g., amine or carboxyl groups). [ 48–50 ] The microarrays shape, diameter, thickness, homogeneity, and binding mechanism are important attributes to evaluate the efficacy of different microarray biosensing surface strategies.…”

Section: Introductionmentioning

confidence: 99%

Antibody Micropatterned Lubricant‐Infused Biosensors Enable Sub‐Picogram Immunofluorescence Detection of Interleukin 6 in Human Whole Plasma

Shakeri

Jarad

Terryberry³

et al. 2020

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Recent studies have shown a correlation between elevated interleukin 6 (IL-6) concentrations and the risk of respiratory failure in COVID-19 patients. Therefore, detection of IL-6 at low concentrations permits early diagnosis of worstcase outcome in viral respiratory infections. Here, a versatile biointerface is presented that eliminates nonspecific adhesion and thus enables immunofluorescence detection of IL-6 in whole human plasma or whole human blood during coagulation, down to a limit of detection of 0.5 pg mL −1. The sensitivity of the developed lubricant-infused biosensor for immunofluorescence assays in detecting low molecular weight proteins such as IL-6 is facilitated by i) producing a bioink in which the capture antibody is functionalized by an epoxy-based silane for covalent linkage to the fluorosilanized surface and ii) suppressing nonspecific adhesion by patterning the developed bioink into a lubricant-infused coating. The developed biosensor addresses one of the major challenges for biosensing in complex fluids, namely nonspecific adhesion, therefore paving the way for highly sensitive biosensing in complex fluids. where significantly elevated levels indicate aggressive tumor growth or viral load and poor prognosis in patients. [2,10] Additionally, IL-6 is an important anti-inflammatory cytokine that induces acute responses in chronic inflammatory pathologies. As such, there has been an increasing interest in the use of IL-6 as a biomarker for the diagnosis of early stages of viral infections, cancer, and chronic inflammation. [9-11] A practical IL-6 biosensor should provide a low limit of detection (LOD) (≤5 pg mL −1) and acceptable linear dynamic range (1-100 pg mL −1) in complex fluids, in addition to accuracy, facile operation, and amenable to mass production. [11,12] There are a large number of different IL-6 detection techniques that have been reported in the literature including electrochemical sensors, [13-22] surface plasmon resonance (SPR), [23-25] chemiluminescence immunoassay (CLIA), [26-29] and immunofluorescence assays (IFA), [30-33] Utilizing 0-and 1-D materials such as carbon nanotubes (CNTs), [14,16] nanoparticles and nanowires, [13,19] as well as porous nanoparticles, [15] optical fibers, [32] and microfluidic platforms, [28] have enabled higher sensitivity in IL-6 detection and to date, electrochemical methods have proven to be the most promising candidate for detection of IL-6 at very low concentrations (0.33 pg mL −1 in buffer) with a wide linear dynamic range. [22] While reported IL-6 biosensors have demonstrated satisfactory LODs in buffer or processed serum, their performance in human whole plasma declines significantly, leading to higher LOD's and/or false positive results. In electrochemical sensors, for example, the nonspecific attachment of biological entities in plasma or blood can interfere with the resistivity at the electrodes thereby deteriorating their sensitivity for detection of IL-6 at clinically relevant concentrations. [34] So far, the lowest theoretical LOD fo...

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Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

Raza,

Poria,

Kala

et al. 2024

Biotech and App Biochem

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Globally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100–300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile sickness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection. Therefore, this review summarizes advances in the detection of dengue from conventional methods (nucleic acid‐based, polymerase chain reaction‐based, and serological approaches) to novel biosensors. This work illustrates an extensive study of the current designs and fabrication approaches involved in the formation of electrochemical biosensors for untimely identifications of dengue. Additionally, in electrochemical sensing of DENV, we skimmed through significances of biorecognition molecules like lectins, nucleic acid, and antibodies. The introduction of emerging techniques such as the CRISPR/Cas’ system and their integration with biosensing platforms has also been summarized. Furthermore, the review revealed the importance of electrochemical approach compared with traditional diagnostic methods.

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Immunofluorescence–based biosensor for the determination of dengue virus NS1 in clinical samples

Cited by 35 publications

References 48 publications

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Development of peptide biosensor for the detection of dengue fever biomarker, nonstructural 1

Antibody Micropatterned Lubricant‐Infused Biosensors Enable Sub‐Picogram Immunofluorescence Detection of Interleukin 6 in Human Whole Plasma

Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches

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