1994
DOI: 10.1083/jcb.125.4.825
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Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae.

Abstract: Abstract. Myo2 protein (Myo2p), an unconventional myosin in the budding yeast Saccharomyces cerevisiae, has been implicated in polarized growth and secretion by studies of the temperature-sensitive myo2-66 mutant. Overexpression of Smylp, which by sequence is a kinesin-related protein, can partially compensate for defects in the myo2 mutant (Lillie, S. H., and S. S. Brown. 1992. Nature (Lond.). 356:358-361). We have now immunolocalized Smylp and Myo2p. Both are concentrated in regions of active growth, as caps… Show more

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Cited by 277 publications
(259 citation statements)
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References 43 publications
(74 reference statements)
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“…Thus, the fluorescence measurement is an indirect indication of the relative amount of F-actin in the cell at the time of fixation. Using this method, Lillie and Brown [17] have shown that a shift to high temperature causes a rapid but transient depolymerization of actin, which is accompanied by a transient redistribution of actin-containing structures [17,19,20]. Similar effects on actin organization have been noted upon increasing the osmotic strength of the medium [211.…”
Section: Resultsmentioning
confidence: 67%
See 2 more Smart Citations
“…Thus, the fluorescence measurement is an indirect indication of the relative amount of F-actin in the cell at the time of fixation. Using this method, Lillie and Brown [17] have shown that a shift to high temperature causes a rapid but transient depolymerization of actin, which is accompanied by a transient redistribution of actin-containing structures [17,19,20]. Similar effects on actin organization have been noted upon increasing the osmotic strength of the medium [211.…”
Section: Resultsmentioning
confidence: 67%
“…The polymerization state of actin filaments can be examined in vivo using the F (filamentous)-actin-specific drug phalloidin conjugated to a fluorochrome such as rhodamine [17]. An indication of the relative amounts of F-actin in a cell can be determined by exposing cells to varying conditions (such as heat shock), followed by rapid fixation at specific time points.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…However, this rescue does not depend on microtubules, nor on critical residues in the P-loop of SMY1. SMY1 localizes to a region lacking microtubules (Lillie and Brown, 1994) and the removal of microtubules has no effect on the SMY1 localization pattern nor on SMY1 cap formation (Lillie and Brown, 1998). However, because microtubules are not essential for the yeast secretory pathway, one cannot rule out that SMY1 may have some microtubule-dependent functions in the secretory pathway.…”
Section: Are There Other Kinesins Like Nod?mentioning
confidence: 99%
“…The myosin-V Myo2p rapidly translocates along actin cables to concentrate at growth sites (Johnston et al, 1991;Lillie and Brown, 1994;Pruyne et al, 1998;Schott et al, 2002). We examined the distribution of Myo2p in wild-type, bni1⌬/ bni1⌬, and bnr1⌬/bnr1⌬ cells by immunofluorescence staining ( Figure 2, A and B).…”
Section: Yeast Lacking Bni1p or Bnr1p Exhibit Myosin-v Transport Defectsmentioning
confidence: 99%