Immunofluorescence of avian infectious bronchitis virus in primary chicken embryo kidney, liver, lung, and fibroblast cell cultures

Pd, Lukert

doi:10.1007/bf01241849

Cited by 30 publications

(10 citation statements)

References 7 publications

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“…Primary chicken embryonic lung (CELu) and liver (CELi) cells were isolated and prepared from specific pathogen-free (SPF) 15-day-old White Leghorn chicken embryos (CAVac Lab. Co., Ltd., Daejeon, Korea) as described elsewhere 17 using a protocol approved by the Medical Research Institute of Chungbuk National University (approval NO CBNU-IRB-2012-GM01). Upon isolation, tissues were minced, trypsinized and cultured at 37 °C in 5% CO 2 in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum, 2 mM glutamine and antibiotics.…”

Section: Methodsmentioning

confidence: 99%

Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus

Kim

Pascua

Kwon

et al. 2014

Emerging Microbes & Infections

114

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The endemicity of highly pathogenic avian influenza (HPAI) A(H5N1) viruses in Asia has led to the generation of reassortant H5 strains with novel gene constellations. A newly emerged HPAI A(H5N8) virus caused poultry outbreaks in the Republic of Korea in 2014. Because newly emerging high-pathogenicity H5 viruses continue to pose public health risks, it is imperative that their pathobiological properties be examined. Here, we characterized A/mallard duck/Korea/W452/2014 (MDk/W452(H5N8)), a representative virus, and evaluated its pathogenic and pandemic potential in various animal models. We found that MDk/W452(H5N8), which originated from the reassortment of wild bird viruses harbored by migratory waterfowl in eastern China, replicated systemically and was lethal in chickens, but appeared to be attenuated, albeit efficiently transmitted, in ducks. Despite predominant attachment to avian-like virus receptors, MDk/W452(H5N8) also exhibited detectable human virus-like receptor binding and replicated in human respiratory tract tissues. In mice, MDk/W452(H5N8) was moderately pathogenic and had limited tissue tropism relative to previous HPAI A(H5N1) viruses. It also induced moderate nasal wash titers in inoculated ferrets; additionally, it was recovered in extrapulmonary tissues and one of three direct-contact ferrets seroconverted without shedding. Moreover, domesticated cats appeared to be more susceptible than dogs to virus infection. With their potential to become established in ducks, continued circulation of A(H5N8) viruses could alter the genetic evolution of pre-existing avian poultry strains. Overall, detailed virological investigation remains a necessity given the capacity of H5 viruses to evolve to cause human illness with few changes in the viral genome.

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Section: Methodsmentioning

confidence: 99%

Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus

Kim

Pascua

Kwon

et al. 2014

Emerging Microbes & Infections

114

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“…CEK cells was prepared and cultured as previously described (Lukert, 1965(Lukert, , 1966. Briefly, primary CEK cells were prepared from the kidneys of 18-20-day-old specific pathogen-free chicken embryos, and maintained in Dulbecco's Modified Eagle Medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Tripathy, 1998).…”

Section: Viruses and Passagingmentioning

confidence: 99%

Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction

Wei¹,

Guo²,

Duan³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Infectious bronchitis virus (IBV) can multiply effectively in chick embryo kidney (CEK) cells after adapting to the chick embryo. To investigate the dynamic changes in IBV load in the supernatant of primary CEK cells, we developed an SYBR Green I-based real-time polymerase chain reaction assay to quantify nucleic copy numbers of the IBV-Sczy3 strain. The 20, 54, and 87th generations of CEK-adapted IBV-Sczy3 strains were used to infect CEK cells, and then nucleic copy numbers in the samples of supernatant collected at 12, 24, 36, 48, 60, and 72 h were detected. The results showed that the rapid growth period of the virus load of all the 3 generations was approximately 12-36 h post- (2015) infection; the peak of the virus load appeared at 36 h post-infection and then decreased gradually in the order of 20th > 54th > 87th for the 3 generations of CEK-adapted strains; the dynamic change curve of the IBV load in the supernatant of primary CEK cells showed a single peak. The results of this study provide a useful reference for CEK-adapted IBV field strains and the production of CEK-attenuated IBV vaccine.

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“…The IFA can also be used for detection of IBV in embryonated eggs (Lucio & Hitchner, 1970;Clarke et al, 1972;Pensaert & Lambrechts, 1994), cell cultures (Mohanty et al, 1964;Lukert, 1966Lukert, , 1969Wainright et al, 1989) and TOCs (Jones, 1973;Bhattecharjee et al, 1994). Using IFA in combination with VI, instead of waiting until the embryo changes occur, decreases the number of passages needed to detect IBV and shortens the number of days per passage from about 7 to 2 days (Clarke et al, 1972).…”

Section: Detection Of Ibv Antigenmentioning

confidence: 99%

“…In general, adaptation of IBV strains is necessary for fluent replication and induction of cytopathic effect (CPE) (Gillette, 1973). Chick embryo kidney (CEK) cells and chicken kidney cells show the highest sensitivity for adapted IBV strains (Lukert, 1965(Lukert, , 1966Otsuki et al, 1979).…”

Section: Virus Isolation (Multiplication and Detection Of Infectious mentioning

confidence: 99%

Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

167

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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Immunofluorescence of avian infectious bronchitis virus in primary chicken embryo kidney, liver, lung, and fibroblast cell cultures

Cited by 30 publications

References 7 publications

Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus

Pathobiological features of a novel, highly pathogenic avian influenza A(H5N8) virus

Dynamic changes of virus load in supernatant of primary CEK cell culture infected with different generations of avian infectious bronchitis virus strains Sczy3 as revealed by real-time reverse transcription-polymerase chain reaction

Detection of infectious bronchitis virus

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