Immunofluorescent localization and extractability of fibronectin in human dental pulp

Amerongen, J.P. van; Lemmens, Inez G.; Tonino, G.J.M.

doi:10.1016/0003-9969(84)90111-0

Cited by 19 publications

(3 citation statements)

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“…FN has been shown to be a constituent of adult human dental pulp (van Amerongen et al, 1984;Karjalainen et al, 1986), whereas its presence in pre-dentin is somewhat controversial. FN has been identified in pre-dentin during early (Thesleff et al, 1979), as well as late (Takita et al, 1987), stages of mouse tooth development and also in the initially formed rat predentin, but not in rat pre-dentin during further stages of odontogenesis (Linde et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

“…The non-mineralized pre-dentin appears to differ from mineralized dentin, particularly in terms of the NCP constitution (for review, see Linde, 1989). The loose connective tissue of the dental pulp contains type I collagen, substantial proportions of type III collagen (Magloire et al, 1982;van Amerongen et al, 1983), and glycoproteins such as fibronectin (van Amerongen et al, 1984). The main constituent of the dental cementum matrix is type I collagen.…”

Section: Introductionmentioning

confidence: 98%

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Immunohistochemical Localization of the Matrix Glycoproteins-Tenascin and the ED-sequence-containing Form of Cellular Fibronectin—in Human Permanent Teeth and Periodontal Ligament

Lukinmaa

Mackie²

1991

J Dent Res

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The expression of two matrix glycoproteins, tenascin and cellular fibronectin (cFN), has been studied in fully developed human permanent teeth, periodontal ligament, and alveolar bone, in both frozen and paraffin-processed material. Polyclonal antibodies to tenascin and a monoclonal antibody recognizing the ED sequence specific to at least some forms of cFN were used. Staining for both tenascin and cFN was positive in the dental pulp, odontoblastic layer, cementoblast-pre-cementum zone, and on the periosteal as well as endosteal surfaces of the alveolar bone. In the periodontal ligament, cFN was evenly distributed, whereas tenascin was accumulated in the attachment zones. Pre-dentin stained for tenascin but not for cFN. Mineralized dentin and cementum were tenascin- and cFN-negative. The relative staining intensity for tenascin was greater than that for cFN in the cementoblast-pre-cementum layer and in the attachment zones of the periodontal ligament, whereas cFN stained more intensely in the pulp. In frozen material, antigenicities were well-preserved. Paraffin processing facilitated precise recognition of tissue morphology, but the antigenicity of cFN was lost. The co-expression of tenascin and cFN in the dental pulp, cementogenic zone, and on the surfaces of the alveolar bone may reflect the ability of the cells to deposit mineralized tissue matrices. The pronounced expression of tenascin in the interfaces between mineralized and non-mineralized tissues suggests that it is functionally associated with mechanical stress and may thus have at least two distinct functions. The relative amounts of the two matrix glycoproteins may contribute to regulation of tissue structure.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Immunohistochemical Localization of the Matrix Glycoproteins-Tenascin and the ED-sequence-containing Form of Cellular Fibronectin—in Human Permanent Teeth and Periodontal Ligament

Lukinmaa

Mackie²

1991

J Dent Res

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“…Outras pesquisas evidenciaram a distribuição de fibronectina na polpa dentária, sendo mais fortemente expressa na interface tecido mineralizado e não-mineralizado (LUKINMAA;THESLEFF, 1991;MARTINEZ et al, 2000;TONINO, 1984). Entretanto, Martinez et al (2000) não observaram a expressão de fibronectina e tenascina nas polpas dentárias com processo inflamatório.…”

Section: Pastas De Hidróxido De Cálciounclassified

Efeitos de fármacos utilizados na terapia endodôntica de dentes decíduos: análise da citotoxicidade e estudo in vitro da distribuição de proteínas da matriz extracelular e do citoesqueleto de fibroblastos da polpa dental humana

Cerqueira¹

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Dentine–Pulp Complex Regeneration

Peters

Gaudin

2021

Regenerative Approaches in Dentistry

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Immunofluorescent localization and extractability of fibronectin in human dental pulp

Cited by 19 publications

References 31 publications

Immunohistochemical Localization of the Matrix Glycoproteins-Tenascin and the ED-sequence-containing Form of Cellular Fibronectin—in Human Permanent Teeth and Periodontal Ligament

Immunohistochemical Localization of the Matrix Glycoproteins-Tenascin and the ED-sequence-containing Form of Cellular Fibronectin—in Human Permanent Teeth and Periodontal Ligament

Efeitos de fármacos utilizados na terapia endodôntica de dentes decíduos: análise da citotoxicidade e estudo in vitro da distribuição de proteínas da matriz extracelular e do citoesqueleto de fibroblastos da polpa dental humana

Dentine–Pulp Complex Regeneration

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