Immunogenic epitopes on the surface of the hepatitis A virus capsid: Impact of secondary structure and/or isoelectric point on chimeric virus assembly

Kusov, Yuri; Gauss‐Müller, Verena; Morace, G

doi:10.1016/j.virusres.2007.06.002

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…We hypothesized that ionic interactions must be involved. The isoelectric points of the NoV GII and the HAV capsids fall, respectively, in the ranges 5.5–6.9 [ 24 ] and 2.8–5.5 [ 25 , 26 ]. At a neutral pH (in CV-1 buffer), these viruses should bear a negative charge and, therefore, bind readily to ApoH.…”

Section: Discussionmentioning

confidence: 99%

Detection of Enteric Viruses on Strawberries and Raspberries Using Capture by Apolipoprotein H

Levesque

Jubinville

Hamon

et al. 2021

Foods

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Human noroviruses (HuNoVs) and the hepatitis A virus (HAV) are the main viral causes of foodborne illness worldwide. These viruses are frequently transmitted via fresh and frozen berries, such as strawberries and raspberries. ISO 15216:1 (2017), currently the preferred method for their detection, involves several steps and is time-consuming. Apolipoprotein H (ApoH) has been shown to have a strong affinity for several microorganisms, including HuNoVs. In this article, we report an ApoH-based method of capturing the HAV and HuNoVs adherent to berries and concentrating them for assay. The limit of detection of both viruses suspended in a buffer was low. On strawberries, the HAV was detected down to 104 genome copies/25 g in 100% of cases and down to 103 genome copies/25 g on raspberries in 50% of cases. This sensitivity was not significantly different from that of the ISO method 15216:1 (2017). HuNoV GII.4 was more difficult to detect using the ApoH method. The ApoH CaptoVIR kit does, nevertheless, appear to be usable in the near future as a single-test, multiple-detection method for viruses on fresh and frozen berries.

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Section: Discussionmentioning

confidence: 99%

Detection of Enteric Viruses on Strawberries and Raspberries Using Capture by Apolipoprotein H

Levesque

Jubinville

Hamon

et al. 2021

Foods

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Concentration of foodborne viruses eluted from fresh and frozen produce: Applicability of ultrafiltration

Trudel-Ferland,

Levasseur,

Goulet-Beaulieu

et al. 2024

International Journal of Food Microbiology

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POTN: A Human Leukocyte Antigen-A2 Immunogenic Peptides Screening Model and Its Applications in Tumor Antigens Prediction

Meng

Sui

et al. 2020

Front. Immunol.

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Whole genome/exome sequencing data for tumors are now abundant, and many tumor antigens, especially mutant antigens (neoantigens), have been identified for cancer immunotherapy. However, only a small fraction of the peptides from these antigens induce cytotoxic T cell responses. Therefore, efficient methods to identify these antigenic peptides are crucial. The current models of major histocompatibility complex (MHC) binding and antigenic prediction are still inaccurate. In this study, 360 9-mer peptides with verified immunological activity were selected to construct a prediction of tumor neoantigen (POTN) model, an immunogenic prediction model specifically for the human leukocyte antigen-A2 allele. Based on the physicochemical properties of amino acids, such as the residue propensity, hydrophobicity, and organic solvent/water, we found that the predictive capability of POTN is superior to that of the prediction programs SYPEITHI, IEDB, and NetMHCpan 4.0. We used POTN to screen peptides for the cancer-testis antigen located on the X chromosome, and we identified several peptides that may trigger immunogenicity. We synthesized and measured the binding affinity and immunogenicity of these peptides and found that the accuracy of POTN is higher than that of NetMHCpan 4.0. Identifying the properties related to the T cell response or immunogenicity paves the way to understanding the MHC/peptide/T cell receptor complex. In conclusion, POTN is an efficient prediction model for screening high-affinity immunogenic peptides from tumor antigens, and thus provides useful information for developing cancer immunotherapy.

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Immunogenic epitopes on the surface of the hepatitis A virus capsid: Impact of secondary structure and/or isoelectric point on chimeric virus assembly

Cited by 3 publications

References 38 publications

Detection of Enteric Viruses on Strawberries and Raspberries Using Capture by Apolipoprotein H

Detection of Enteric Viruses on Strawberries and Raspberries Using Capture by Apolipoprotein H

Concentration of foodborne viruses eluted from fresh and frozen produce: Applicability of ultrafiltration

POTN: A Human Leukocyte Antigen-A2 Immunogenic Peptides Screening Model and Its Applications in Tumor Antigens Prediction

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