Immunohistochemical and immunofluorescent techniques

Sanderson, Tracy; Wild, Graeme; Cull, Ann; Marston, Jennifer; Zardin, Greg

doi:10.1016/b978-0-7020-6864-5.00019-0

Cited by 20 publications

(12 citation statements)

References 72 publications

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“…OSMF, which was previously known as a premalignant condition, is now categorized under OPMD; OSMF has a prevalence rate of 0.2% in Gujarat-0.5% in Kerala,[ 10 ] with a malignant transformation rate of 7.6%[ 11 ] among Indian villagers.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical detection of 8-hydroxydeoxyguanosine: A biomarker of oxidative DNA damage in oral submucous fibrosis

Kulasekaran

Devi²,

Dhivya³

et al. 2020

J Oral Maxillofac Pathol

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical detection of 8-hydroxydeoxyguanosine: A biomarker of oxidative DNA damage in oral submucous fibrosis

Kulasekaran

Devi²,

Dhivya³

et al. 2020

J Oral Maxillofac Pathol

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“…Goblet cell count was done by counting goblet cells per crypt in a minimum of 10 crypts in each group 28 . The standard immunohistochemical staining protocol by Sanderson et al 29 was applied to determine the immunoreactivity of mTOR and LC3B. Anti‐mTOR antibody was purchased from Fine Test (Cat# FNab05417, RRID: AB_2923348, 1:200 dilution).…”

Section: Methodsmentioning

confidence: 99%

Vitamin D and lactoferrin attenuate stress‐induced colitis in Wistar rats via enhancing AMPK expression with inhibiting mTOR–STAT3 signaling and modulating autophagy

Abdelmalak

Abdelrahim

Michael

et al. 2023

Cell Biochemistry & Function

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Irritable bowel syndrome (IBS) is a global gastrointestinal disorder closely related to psychological stress exposure and local colonic inflammation. Herein, we investigated the effect of wrap‐restraint stress (WRS) on rat behavior, on adenosine monophosphate‐activated protein kinase–mammalian/mechanistic target of rapamycin–signal transducer and activator of transcription 3 (AMPK–mTOR–STAT3) signaling, and autophagy in colonic mucosa. The impact of chronic administration of vitamin D3 and lactoferrin was compared. Twenty‐four male Wistar rats were randomly divided into four groups. Chronic WRS protocol was applied as a rodent model of IBS. Group I: naïve animals, Group II: WRS animals, Group III: WRS‐exposed and treated with vitamin D3 (500 IU/kg/day), and Group IV: WRS‐exposed and treated with lactoferrin (300 mg/kg/day). In this study, we found that chronic administration of each of vitamin D3 and lactoferrin resulted in a significant increase in social interaction test, interleukin‐10, AMPK, optical density of LC3B, goblet cell count and marked decrease in serum cortisol level, STAT3, inflammatory cell count, and optical density of mTOR in comparison to the WRS rats. Our findings suggest that both vitamin D3 and Lactoferrin could augment colonic autophagy through enhanced AMPK expression and inhibition of mTOR–STAT3 signaling, which offers practical insights into their clinical use in the prevention and therapy of IBS. However, lactoferrin intake as a nutritional supplement could be more helpful for stress‐induced colitis treatment than vitamin D3.

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“…Avidin-biotin complex technique was used [ 27 ]. Samples were riding on positively charged slides, deparaffinization and rehydration were performed.…”

Section: Methodsmentioning

confidence: 99%

Role of nuclear factor-kappa B in bleomycin induced pulmonary fibrosis and the probable alleviating role of ginsenoside: histological, immunohistochemical, and biochemical study

et al. 2021

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Bleomycin (BLM) is one of anti-cancerous drugs. One of its limitation is the development of pulmonary fibrosis during therapy So, we proposed to examine the outcome of BLM take on the light and electron microscopic design of rat lung. Along with, assessment the probable protecting role of ginsenoside on BLM induced pulmonary changes. In this study, thirty adult male albino rats were comprised and were classified to four clusters; Negative & positive control group, BLM treated group and BLM& ginsenoside treated group. The lung was treated for histological and immunohistochemical (anti-p65) studies. Light microscopic examination of H&E stained sections of BLM treated group showed huge distortion of the lung building. Mallory trichrome stain of this group showed evident deposition of collagen fibers in the markedly thickened interalveolar septa and around intrapulmonary bronchi, bronchioles and blood vessels. Moreover, strong positive staining for nuclear factor (NF)-κB in the wall of bronchiole as well as the thickened interalveolar septa were observed. Ultrastructural inspection of lung of this group revealed muddled lung planning. Marked improvement of the lung structure and marked reduction in NF-κB immunoexpression was appeared in BLM and ginsenoside treated group. So, we concluded that co-administration of ginsenoside with BLM significantly enhanced the histological and morphometric image of the lung.

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Immunohistochemical and immunofluorescent techniques

Cited by 20 publications

References 72 publications

Immunohistochemical detection of 8-hydroxydeoxyguanosine: A biomarker of oxidative DNA damage in oral submucous fibrosis

Immunohistochemical detection of 8-hydroxydeoxyguanosine: A biomarker of oxidative DNA damage in oral submucous fibrosis

Vitamin D and lactoferrin attenuate stress‐induced colitis in Wistar rats via enhancing AMPK expression with inhibiting mTOR–STAT3 signaling and modulating autophagy

Role of nuclear factor-kappa B in bleomycin induced pulmonary fibrosis and the probable alleviating role of ginsenoside: histological, immunohistochemical, and biochemical study

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