Immunohistochemical Assessment of SOX2 Expression in Iraqi Patients with Gastric Carcinoma (GC)

Ashour, Hanan Jameel; Abdulghafour, Kifah H.; Al-Bakri, Nahla A.

doi:10.5958/0973-9130.2019.00049.5

Cited by 2 publications

(2 citation statements)

References 11 publications

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“…This study also recorded that the moderately differentiated subtype was found in a higher ratio than the poorly differentiated subtype, which has similarity to the results of another study [29]. These results also correspond to those of Ashour et al, [30], who stated that the moderately differentiated subtype was the most common among all cases (about 63.3%). In contrast, the study of Zhang et al [31] found that the poorly differentiated subtype was dominant.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of PARP-1 by immunohistochemistry in a sample of Iraqi patients with gastric cancer

Mwafaq

Abbas

Abdullah

2022

eijs

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PARP-1 is a protein enzyme with a major role in DNA repair that is overexpressed in many malignancies. It is correlated with susceptibility and metastasis to lymph nodes in gastric cancer (GC). The objective of the present investigation is to estimate PARP1 expression in patients with gastric cancer and detected if it could be used as a predictive marker. Furthermore, we aimed to find the correlation between PARP1 expression and clinicopathological parameters, such as gender, age, invasion depth, histopathological type, involvement of lymph nodes, grade, and stages of GC. This is a retrospective study from the period 2018-2020. Fifty randomly selected subjects (10 normal and 40 GC) were examined for formalin-fixed, paraffin-embedded blocks (FFPE) of stomach tissue . The diagnosis reports were collected from the Pathology Department of the Gastroenterology and Hepatology Teaching Hospital and some private hospitals in Baghdad, Iraq. Hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining of PARP1 were applied for the histological sections. Statistical analysis was accomplished by SPSS system at P<0.05. There were significant differences between the patients and control groups in the expression level of PARP1. There were also significant correlations between PARP1 expression and each of the histopathological subtype, grade, invasion depth, involvement of lymph node, and stages in patients. However, non- significant associations were found between the expression and the age and gender of patients. These results indicate that PARP1 could be employed as a good prospective marker for gastric cancer.

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Section: Discussionsupporting

confidence: 91%

Evaluation of PARP-1 by immunohistochemistry in a sample of Iraqi patients with gastric cancer

Mwafaq

Abbas

Abdullah

2022

eijs

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“…Immunohistochemistry: Fixed liver specimens were sectioned after following histological protocol as described by AL-Musawi et al (13) and Ashour et al (14) , HRP/DAB IHC detection kit (UK, Abcam) was used according to the manufacturer's procedure.…”

Section: Liver Total Metallothionein (Mt1 and Mt2mentioning

confidence: 99%

Metallothionein Protein Concentration in The Liver Tissue of Albino Mice Exposed to Cadmium and Zinc Chloride

Bayram

Al-Bakri

Al-Shmgani

2022

The Egyptian Journal of Hospital Medicine

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Background: Cadmium (Cd), one of the most abundant heavy metals, is extremely toxic to both humans and animals. hIt is well known that zinc (Zn) administration reduces Cd-induced toxicity and that metallothioneins can have a protective effect in biological systems to mitigate Cd toxicity. Objective: The aim of the current study to determine if Zn administration affected the induction of MT-1 and MT-2 in the liver tissue in mice exposed to Cd. Materials and methods: Metallothionein protein (MT) level in the tissue of male mice were detected using the anionexchang high-performance liquid chromatography coupled (HPLC)assay and immunohistochemical staining. Results: Single treatment to zinc or cadmium increase the level of MT in the liver, but zinc chloride treated significantly increase the level of MT after sub chronic treatment. Conclusion: Zinc pre-treatment with increasing the concentration of the dose of cadmium used in the co-treatment, and both of them may have worked together to induce a significant increase in protein synthesis to exceed the high toxicity of cadmium, by inducing an increase in MT protein synthesis.

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Immunohistochemical Assessment of SOX2 Expression in Iraqi Patients with Gastric Carcinoma (GC)

Cited by 2 publications

References 11 publications

Evaluation of PARP-1 by immunohistochemistry in a sample of Iraqi patients with gastric cancer

Evaluation of PARP-1 by immunohistochemistry in a sample of Iraqi patients with gastric cancer

Metallothionein Protein Concentration in The Liver Tissue of Albino Mice Exposed to Cadmium and Zinc Chloride

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