Immunohistochemical Characteristics of Normal Canine Eyes

Abstract

Immunohistochemistry is widely utilized in diagnostic laboratories to study neoplastic and nonneoplastic diseases. Knowledge of the immunohistochemical characteristics of normal tissue is essential for interpretation of immunoreactivity in pathologic conditions. In this study, immunohistochemistry was performed with a broad panel of diagnostically relevant antibodies on 4 normal canine globes--namely, vimentin, pan-cytokeratin (AE1/AE3), cytokeratin 7, cytokeratin 8/18, cytokeratin 20, α-smooth muscle actin, muscle specific actin, desmin, Melan-A, microphthalmia transcription factor, S-100, glial fibrillary acidic protein, triple neurofilaments, neuron-specific enolase, chromogranin A, synaptophysin, laminin and CD31. Results include cytokeratin immunoreactivity limited to the conjunctival epithelium, corneal epithelium, and retinal pigment epithelium; distinct patterns of immunopositivity of muscle markers; and widespread immunoreactivity for vimentin and most neural/neuroendocrine markers. These findings in normal eyes provide the basis for interpretation of ocular immunohistochemistry in dogs. Published immunophenotypes of primary ocular neoplasms are also reviewed.

Clinical and histologic description of ocular anatomy in captive black‐tailed prairie dogs (Cynomys ludovicianus)

“…As IHC analysis has never been reported in the eye of the PD, these stains were performed to establish a baseline for tumor diagnosis by providing information on how normal ocular structures label with particular IHC stains. Immunohistochemical positive staining in the PD globe occurred in anatomic locations similar to domestic species …”

Comparative morphological evaluation of domestic animal cornea

“…Additionally, intermediate and basal layers of the corneal epithelium showed variable positive CK immunoreactivity in carnivores, and to a lesser extent horse (for CK1), with the antibodies used. Labelle et al . demonstrated that all cell layers of dog corneal epithelium were immunopositive for pan‐CK and CK8/18 antibodies.…”

Advanced Clinical Imaging and Tissue-based Biomarkers of the Eye for Toxicology Studies in Minipigs

“…To the best of our knowledge, we demonstrate for the first time a panel of tissue-based IHC or ISH markers for neuronal and glial cells, photoreceptors, synapses, RPE, macrophages, and vasculature and cell proliferation in DF-fixed paraffin-embedded eyes in minipigs while preserving good morphology. The following IHC markers were successfully established on DF-fixed porcine eyes and revealed the specific staining of cells or structures they were described to identify according to the published data: (Reddy et al 2009); RPE65 for RPE (FernandezBueno et al 2013); vWF for blood vessel endothelia (Rand et al 1987); aSMA for smooth muscle cells and myofibroblasts in blood vessels (Umazume et al 2012); desmin for type III intermediate filaments expressed in muscle cells (Labelle et al 2012); and Ki-67 for cell proliferation (Sawhney and Hall 1992).…”