Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature

Iino, Satoshi; Nojyo, Yoshiaki

doi:10.1679/aohc.72.107

Cited by 59 publications

(95 citation statements)

References 21 publications

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“…CD34‐positive telocytes that do not express c‐Kit may also perform a pacemaker function 48, 49 via c‐Kit‐independent pathways, such as the SK3 pathway, by inhibiting electrical activity. This is unlike the stimulating pathways activated by c‐Kit 20, 23, and therefore may play a complementary role to the pacemaker activity exerted by c‐Kit‐positive telocytes or ICCs.…”

Section: Discussionmentioning

confidence: 98%

“…ICCs, described in organs of the digestive system, such as the stomach and intestine, possess pacemaker activity via the c‐kit receptor 19, 20, 21, the ligand of which is the stem cell factor (SCF), that acts in a way to facilitate calcium mobilization via a src family kinase and PI3K (Phosphatidylinositol‐4,5‐bisphosphate 3‐kinase) 22. Telocytes may also exercise pacemaker function by means of the c‐Kit, but may also possess a supportive function for the contraction of periductal smooth muscle, possibly by means of c‐kit‐independent pathways, such as via the SK3 (small conductance calcium‐activated potassium Channel 3) pathway 20, 23. The function of telocytes varies between organs 8, 10, 11, 12, 17, 18, 24, 25, and their role in the prostate remains elusive, in addition to lack of information regarding the development of these cells in prostate tissue.…”

Section: Introductionmentioning

confidence: 99%

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Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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Telocytes are CD34‐positive interstitial cells, known to exert several functions, one of which is a role in tissue organisation, previously demonstrated by telocytes in the myocardium. The existence of telocytes in the prostate has recently been reported, however, there is a lack of information regarding the function of these cells in prostate tissue, and information regarding the possible role of these cells in prostatic development. This study used immunofluorescence techniques in prostate tissue and prostatic telocytes in culture to determine the relationship between telocytes and prostate morphogenesis. Furthermore, immunofluorescent labelling of telocytes was performed on prostate tissue at different stages of early postnatal development. Initially, CD34‐positive cells are found at the periphery of the developing alveoli, later in the same region, c‐kit‐positive cells and cells positive for both factors are verified and CD34‐positive cells were predominantly observed in the interalveolar stroma and the region surrounding the periductal smooth muscle. Fluorescence assays also demonstrated that telocytes secrete TGF‐β1 and are ER‐Beta (ERβ) positive. The results suggest that telocytes play a changing role during development, initially supporting the differentiation of periductal and perialveolar smooth muscle, and later, producing dense networks that separate alveoli groups and form a barrier between the interalveolar region and periurethral smooth muscle. We conclude that telocytes play a relevant role in prostate tissue organisation during postnatal development.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Sanches

Maldarine

Zani

et al. 2017

J Cellular Molecular Medi

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“…In humans, although all the CD34 positive cells were found to be also PDGFRα + , in the axes of the villi there were described only as PDGFRα + cells (51,54,55,63). In the muscle wall of humans and rodents the PDGFRα + cells also expressed the SK3 channel (see above) but, in the mouse, these PDGFRα/SK3 + cells were CD34 negative (60). In summary, also in the GI tract, TC show regions and/ or species differences.…”

Section: Tc Subtypesmentioning

confidence: 94%

“…Several groups of researcher have shown that in the gut of rodents and humans the PDGFRα + cells also express the small conductance Ca 2+ -activated K + channel 3 (SK3) (53,55,(60)(61)(62). It was also ascertained that none of the c-Kit positive cells expressed the SK3 and, in ICC deficient mouse strains, the channel expression was preserved (53,55,(60)(61)(62). Table 2)…”

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

“…However, because of the vagueness of this indirect definition of the cell identity, also, this name has been gradually abandoned and in the most recent papers these cells are simply called PDGFRα + cells (54,55,(61)(62)(63). Several groups of researcher have shown that in the gut of rodents and humans the PDGFRα + cells also express the small conductance Ca 2+ -activated K + channel 3 (SK3) (53,55,(60)(61)(62). It was also ascertained that none of the c-Kit positive cells expressed the SK3 and, in ICC deficient mouse strains, the channel expression was preserved (53,55,(60)(61)(62).…”

Section: Tc Identification By Immunohistochemistry (See Table 1)mentioning

confidence: 99%

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Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

Vannucchi

Traini

2016

Biomolecular Concepts

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Abstract:In the interstitium of the connective tissue several types of cells occur. The fibroblasts, responsible for matrix formation, the mast cells, involved in local response to inflammatory stimuli, resident macrophages, plasma cells, lymphocytes, granulocytes and monocytes, all engaged in immunity responses. Recently, another type of interstitial cell, found in all organs so far examined, has been added to the previous ones, the telocytes (TC). In the gut, in addition to the cells listed above, there are also the interstitial cells of Cajal (ICC), a peculiar type of cell exclusively detected in the alimentary tract with multiple functions including pace-maker activity. The possibility that TC and ICC could correspond to a unique cell type, where the former would represent an ICC variant outside the gut, was initially considered, however, further studies have clearly shown that ICC and TC are two distinct types of cells. In the gut, while the features and the roles of the ICC are established, part of the scientific community is still disputing these 'new' interstitial cells to which several names such as fibroblast-like cells (FLCs), interstitial Cajal-like cells or, most recently, PDGFRα + cells have been attributed. This review will detail the main features and roles of the TC and ICC with the aim to establish their relationships and hopefully define the identity of the TC in the gut.

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The Mandibular Ridge Oral Mucosa Model of Stromal Influences on the Endothelial Tip Cells: An Immunohistochemical and TEM Study

Rusu

Didilescu

Stănescu

et al. 2012

The Anatomical Record

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This study aimed to evaluate by immunohistochemistry and transmission electron microscopy (TEM) the morphological features of the oral mucosa endothelial tip cells (ETCs) and to determine the immune and ultrastructural patterns of the stromal nonimmune cells which could influence healing processes. Immune labeling was performed on bioptic samples obtained from six edentulous patients undergoing surgery for dental implants placement; three normal samples were collected from patients prior to the extraction of the third mandibular molar. The antibodies were tested for CD34, CD117(c-kit), platelet derived growth factor receptor-alpha (PDGFR-a), Mast Cell Tryptase, CD44, vimentin, CD45, CD105, alpha-smooth muscle actin, FGF2, Ki67. In light microscopy, while stromal cells (StrCs) of the reparatory and normal oral mucosa, with a fibroblastic appearance, were found positive for a CD34/ CD44/CD45/CD105/PDGFR-a/vimentin immune phenotype, the CD117/c-kit labeling led to a positive stromal reaction only in the reparatory mucosa. In TEM, non-immune StrCs presenting particular ultrastructural features were identified as circulating fibrocytes (CFCs). Within the lamina propria CFCs were in close contact with ETCs. Long processes of the ETCs were moniliform, and hook-like collaterals were arising from the dilated segments, suggestive for a different stage migration. Maintenance and healing of oral

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Immunohistochemical demonstration of c-Kit-negative fibroblast-like cells in murine gastrointestinal musculature

Cited by 59 publications

References 21 publications

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Telocytes play a key role in prostate tissue organisation during the gland morphogenesis

Interstitial cells of Cajal and telocytes in the gut: twins, related or simply neighbor cells?

The Mandibular Ridge Oral Mucosa Model of Stromal Influences on the Endothelial Tip Cells: An Immunohistochemical and TEM Study

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