Immunohistochemical Detection of Estrogen Receptor in Invasive Human Breast Cancer: Correlation with Heat Shock Proteins, pS2 and Oncogene Products

Takahashi, Shuji; Narimatsu, Eimei; Asanuma, Hiroko; Okazaki, Masanori; Okazaki, Atsushi; Hirata, Koichi; Mori, Makoto; Chiba, Takayuki; Sato, Noriyuki; Kikuchi, Kokichi

doi:10.1159/000227491

Cited by 24 publications

(18 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our study did not show any correlation between Hsp27 expression and the expression of ER and PR. Our data is in agreement with similar results obtained by others [26,[35][36][37][38], although the study by Ciocca et al described a positive correlation between Hsp27 and ER [16]. This discrepancy needs further investigation.…”

Section: Discussionsupporting

confidence: 92%

Hsp-27 Expression in Invasive Ductal Breast Carcinoma

Grzegrzółka¹,

Kurnol

Piotrów

et al. 2012

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Abstract:The aim of this study was to determine the intensity of Hsp27 protein expression in fibrocystic breast changes (FC) and invasive ductal breast carcinoma (IDC) and to examine its impact on patients' clinico-pathological characteristics and overall survival. Immunohistochemical reactions were conducted on archival samples of 20 cases of FC and 101 cases of IDC treated in 1999-2002. Nuclear-cytoplasmic Hsp27 expression was observed in 92 (92.1%) of the examined cases of IDC, and all the cases of FC. Significantly higher Hsp27 expression was observed in G2 (p < 0.01) and G3 cases (p < 0.0001) compared to FC. HER-2 positive cases had higher Hsp27 expression (p = 0.0153), than HER-2 negative cases. Our research showed that Hsp27 could have an impact on tumor malignancy. Moreover, a positive correlation between the expression of Hsp27 and HER-2 positive cases was demonstrated.

show abstract

Section: Discussionsupporting

confidence: 92%

Hsp-27 Expression in Invasive Ductal Breast Carcinoma

Grzegrzółka¹,

Kurnol

Piotrów

et al. 2012

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

show abstract

“…This has led to the investigation of Hsp27, like ER␣, as a human breast cancer tumor marker with Hsp27 tumor expression currently suggested to be a "downstream" indicator of estrogen-ER␣ interaction in tumor cells (55). In some, but not all studies (63,64), Hsp27 expression has been shown to correlate with ER␣ expression. Therefore, it is of note that in the coimmunoprecipitation, yeast two-hybrid and GST pull-down assays carried out as part of the current studies (see Figs.…”

Section: Discussionmentioning

confidence: 99%

An Hsp27-related, Dominant-negative-acting Intracellular Estradiol-binding Protein

Chen

Hewison

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

New World primates (NWPs) exhibit a compensated form of resistance to gonadal steroid hormones. We demonstrated recently that estrogen resistance in NWP cells was associated with the overexpression of two proteins, a nonreceptor-related, dominant-negative-acting estrogen response element (ERE)-binding protein (ERE-BP) and an intracellular estradiol-binding protein (IEBP). Based on the N-terminal sequences of tryptic fragments of IEBP isolated from a 17␤-estradiol (E 2 ) affinity column we cloned a full-length cDNA for IEBP from the estrogen-resistant NWP cell line, B95-8. Subsequent sequence analysis revealed 87% sequence identity between the deduced peptide for IEBP and human Hsp27. When hormone-responsive, wild-type Old World primate (OWP) cells were transiently transfected with IEBP cDNA, E 2 -directed ERE reporter luciferase activity was reduced by 50% compared with vector only-transfected OWP cells (p < 0.0018). When IEBP and ERE-BP were cotransfected, ERE promoter-reporter activity was reduced by a further 60% (p < 0.0001). Electrophoresis mobility shift analyses showed that IEBP neither bound to ERE nor competed with the estrogen receptor (ER) for binding to ERE. However, there was evidence of protein-protein interaction of IEBP and ER␣; IEBP was coimmunoprecipitated with anti-ER␣ antibody in wildtype cells stably transfected with IEBP. A specific interaction between ER␣ and IEBP was confirmed in glutathione S-transferase pull-down and yeast two-hybrid assays. Data indicate that the Hsp27-related IEBP interacts with the ligand binding domain of the ER␣. In summary, by inhibiting the ER␣-E 2 interaction, IEBP acts to squelch ER␣-directed ERE-regulated transactivation and promote estrogen resistance in NWP cells.Compared with Old World primates (OWPs), 1 including man, New World primates (NWPs) represent an evolutionarily distinct infraorder of primates confined to the South and Central American subcontinents. A central point of distinction between NWPs and OWPs resides in the fact that NWPs display relative resistance to adrenal, gonadal, and vitamin D sterol/steroid hormones (1-11). The precise mechanism for this remains unclear but does not involve aberrant expression of nuclear receptors for specific steroid hormones (12, 13), the principal cause of hormone resistance in humans (14). Instead, hormone resistance in NWP cells appears to be the result of epigenetic factors, which result either in low affinity receptorsteroid binding kinetics or attenuation of receptor-DNA interaction. For example, studies of glucocorticoid resistance in NWP cells have shown increased expression of the heat shock protein (Hsp)90-associated FK506-binding immunophilin FKBP51, which inhibits ligand binding to glucocorticoid receptors (GR) (15). By contrast, vitamin D resistance in NWPs appears to be caused by aberrant expression of a dominantnegative-acting vitamin D response element-binding protein (VDRE-BP), the protein being homologous to human heterogeneous nuclear ribonucleoprotein A (hnRNPA) (16). Estrogen resistance in ...

show abstract

“…At present there are a total of four known members of this type I growth factor receptor gene family (reviewed by Révillion et al) (78). Amplification of the c-erbB-2 gene or increased protein expression correlates with oestrogen receptor negativity (79)(80)(81)(82)(83)(84)(85)(86)(87)(88). Similarly, in 11 out of 20 studies, using univariate models, c-erbB-2 positivity was associated with a statistically significant shorter overall survival (reviewed by Révillion et al) (78,86).…”

Section: C-erbb-2mentioning

confidence: 99%