Immunohistochemical Detection of Proliferating Cell Nuclear Antigen (Pcna)/Cyclin in Human Prostate Adenocarcinoma

Abstract

Tissue specimens from 12 patients with adenocarcinoma of the prostate and 7 patients with benign prostate hypertrophy were stained by an indirect immunoperoxidase method using antiproliferating cell nuclear antigen (PCNA) monoclonal antibody. The PCNA labeling index was determined by counting the number of PCNA-labeled cells in the tissue sections. Average PCNA labeling index of the benign prostate hypertrophy was 1.2 +/- 0.5%. Poorly differentiated tumors averaged 7.6 +/- 3.9% labeling versus 4.6 +/- 1.3% in moderately differentiated tumors, and well differentiated tumor in the series had a PCNA labeling index of 2.5 +/- 0.9%. The PCNA labeling indices for atypical hyperplasia were 1.9, and 4.1%, respectively. Our preliminary results suggest that the measurement of PCNA labeling index in prostate cancer may prove to be a new objective and quantitative assay of biological potential of individual tumor.

Metastasis induced by overexpression of p185neu-T after orthotopic injection into a prostatic epithelial cell line (NbE)

“…Because many prostatic tumors have an increased rate of proliferation relative to surrounding tissue [22,23], the incorporation of [ 3 H]thymidine and constitutive levels of the proliferation marker ODC were compared in the control and the NbE-neu-T clones. All four clones demonstrated an increase in [ 3 H]thymidine incorporation between days 1 and 7 of the study (P ≤ 0.001; Figure 5A).…”

“…Proliferation of prostatic carcinoma increases during disease progression [22,23], and initially, this proliferation is dependent on the presence of androgens. However, during the hormone-insensitive phase of the disease, proliferation must be regulated or stimulated by other as-yet-unidentified pathways.…”

Proliferating Cell Nuclear Antigen in Needle Biopsy Specimens of Prostatic Carcinoma

“…Visakorpi [17] mentioned that there was no close correlation between the PCNA index and glandular differentiation, whereas Nemoto et al [18] reported a good correlation between them. Harper et al [16] also reported that the PCNA index was associated with the Gleason grade.…”

“…A number of malignant tumors have been studied with the PCNA antibody [9][10][11][16][17][18], and it has been dem onstrated that in some malignant tumors the PCNA index is closely correlated with prognosis [9,10,16]. In this study, we also compared the PCNA index of prostatic car cinomas with both the clinicopathological characteristics and prognosis.…”

An evaluation of the markers p53 and Ki-67 for their predictive value in prostate cancer

“…Many prognostic markers have been evaluated [6][7][8]. Two, p53 and Ki-67, have recently received increased attention and are the focus of this report.…”