Immunohistochemical identification of the corticotropin releasing factor (CRF)-containing nerve fibers in the pig hypophysis, with special reference to the relationship between CRF and posterior lobe hormones.

Effect of Hypertonic Saline on the Corticotropin-Releasing Hormone and Arginine Vasopressin Content of the Rat Pituitary Neurointermediate Lobe.

“…The present data appear to indicate that the transport of AVP and CRH from the magnocells to the posterior pituitary is stimulated and that AVP release into the general circulation is stimulated but CRH release is not. Kawata et al(1983) reported that the distribution of the CRHcontaining nerves in the posterior pituitary was found to be similar to that of the vasopressin-containing nerve terminals These results might indicate that CRH in the NIL has a paracrine effect on the AVP secretion, although it is difficult to explain the mechanism of inverse changes in CRH and AVP content in the NIL. Very recently, when this manuscript was in preparation, Jessop et al(1989) reported a similar result: that CRH in the NIL increased after salt loading.…”

“…However, the cell bodies of CRH neurons that project to the external layer of the median eminence (ME) to regulate ACTH secretion are concentrated mainly in the parvocellular division of the hypothalamic paraventricular nucleus (PVN) (Olschowka et al, 1982;Antoni et al, 1983;Swanson et al, 1983;Niimi et al, 1988). CRH has also been detected in the magnocellular cells of both the PVN and the supraoptic nucleus (SON) (Kawata et al, 1982;Burlet et al, 1983), and in the posterior pituitary (Kawata et al, 1983;Hashimoto et al, 1984;Suda et al, 1984.;Jeandel et al, 1987). We previously reported that CRH immunoreactive nerve terminals are distributed around the blood vessels in the pig posterior lobe, particularly in the proximal adjacent to the intermediate lobe (Kawata et al, 1983) and that the CRH present in the neurointermediate lobe of the rat pituitary gland (NIL) is mainly composed of authentic rat CRH (1-41).…”

“…CRH has also been detected in the magnocellular cells of both the PVN and the supraoptic nucleus (SON) (Kawata et al, 1982;Burlet et al, 1983), and in the posterior pituitary (Kawata et al, 1983;Hashimoto et al, 1984;Suda et al, 1984.;Jeandel et al, 1987). We previously reported that CRH immunoreactive nerve terminals are distributed around the blood vessels in the pig posterior lobe, particularly in the proximal adjacent to the intermediate lobe (Kawata et al, 1983) and that the CRH present in the neurointermediate lobe of the rat pituitary gland (NIL) is mainly composed of authentic rat CRH (1-41). (Hashimoto et al, 1984).…”