Immunohistochemical Localization of CYP1A-Like and CYP3A-like Isozymes in Hepatic and Extrahepatic Tissues of Atlantic Cod (Gadus morhua L), a Marine Fish

Husøy, Astrid-Mette; Myers, Mark S.; Willis, Maryjean L.; Collier, Tracy K.; Celander, Malin; Goksøyr, Anders

doi:10.1006/taap.1994.1254

Cited by 112 publications

(36 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This decrease was mediated by the bioactivation of MeSO 2 -DDE by adrenal cytochrome P450c11 (a key mitochondrial enzyme involved in cortisol synthesis), resulting in the inhibition of steroid hydroxylase, which is on the final pathway of corticosterone biosynthesis (Lund & Lund 1995). As BNF is a potent inducer of P450s in fish tissues, including interrenals (Husøy et al 1994), it is possible that substrate competition by xenobiotics in the steroid biosynthetic pathway may result in lower cortisol production. Plasma cortisol concentration was higher in the cannulated fish than in the resting (uncannulated) fish regardless of BNF treatment (Table 1).…”

Section: Discussionmentioning

confidence: 99%

beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

Wilson¹,

Vijayan²,

Kennedy³

et al. 1998

Journal of Endocrinology

View full text Add to dashboard Cite

We report for the first time that -naphthoflavone (BNF) abolishes ACTH stimulation of cortisol production in rainbow trout (Oncorhynchus mykiss). There was significantly higher hepatic cytochrome P450 content and ethoxyresorufin O-de-ethylase and uridine-5 -diphosphoglucuronic acid transferase activities in BNFtreated fish than in sham-treated controls. BNF did not significantly effect either plasma turnover or tissue distribution of [ 3 H]cortisol-derived radioactivity. Hepatic membrane fluidity and hepatocyte capacity for cortisol uptake were not altered by BNF as compared with the sham-treated fish. These results taken together suggest that BNF does not affect cortisol-clearance mechanisms in trout. A 3 min handling disturbance period elicited a plasma cortisol response in the sham-treated fish; however, the response in the BNF-treated fish was muted and significantly lower than in the sham fish. This in vivo response corroborates the lack of interrenal sensitivity to ACTH in vitro in the BNF-treated fish, suggesting that BNF affects the ACTH pathway in trout. Our results suggest the possibility that cytochrome P450-inducing compounds may affect cortisol dynamics by decreasing interrenal responsiveness to ACTH stimulation in fish, thereby impairing the physiological responses that are necessary for the animal to cope with the stressor.

show abstract

Section: Discussionmentioning

confidence: 99%

beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

Wilson¹,

Vijayan²,

Kennedy³

et al. 1998

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…All three CYP isoforms seem to be involved in AFB 1 biotransformation in trout (detailed later). Other than the families CYP2 and CYP3, the CYP1A subfamily, which represents inducible genes via aryl hydrocarbon receptor (AhR) (Dogra et al 1998), was found to be expressed in most fish species at low constitutive levels in hepatic tissue, although they also can be found in gill, kidney, gut and heart tissues following induction (Stegeman 1989;Husoy et al 1994).…”

Section: Phase I Cypsmentioning

confidence: 99%

Aflatoxins in aquatic species: metabolism, toxicity and perspectives

Santacroce

Conversano

Casalino

et al. 2007

Rev Fish Biol Fisheries

159

119

View full text Add to dashboard Cite

Among all known mycotoxins, aflatoxins represent the most investigated, widespread and worrisome source of contamination of foods and feed worldwide. In the early 1960s, soon after the finding of aflatoxin B 1 (AFB 1 ) in the feedstuffs of aquacultured rainbow trout that had died in an epizootic of hepatomas, great scientific discoveries were made in several areas by a number of researchers under the direction of scientists like J. Halver, R. 0. Sinnhuber, G. S. Bailey, J. D. Hendricks and colleagues. Since that time, several studies have focused on the identification of new isoenzymes involved in AFB 1 metabolism and on the discovery of new modulators in AFB 1 -induced cancer initiation and progression. However, metabolic and toxicological studies on aflatoxins in marine aquacultured species are fragmented and restricted to a limited number of fish species. Aflatoxins exert a substantial impact on the fish farming production, causing disease with high mortality and a gradual decline of reared fish stock quality, thus representing a significant problem in aquaculture systems. Based on these considerations, the goals of this review article are: (1) to gather the currently available scientific information, summarising existing data on aflatoxin contamination on feeds and fishmeals, and toxicological effects induced in reared aquatic species; (2) to make a comparative analysis of AFB 1 metabolism in the most representative species studied; (3) to gain new insights on the risk of DNA damage caused by aflatoxins on fish genomes and their role in cancer development.

show abstract

“…Tissue samples were processed [31] and the samples embedded in paraffin [32]. An indirect peroxidase method was used for immunohistochemical staining [33].…”

Section: Cyp1a-associated Expressionmentioning

confidence: 99%

Biomarker responses and disease susceptibility in juvenile rainbow trout Oncorhynchus mykiss fed a high molecular weight PAH mixture

Bravo

Curtis

Myers

et al. 2011

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Juvenile rainbow trout were fed a diet containing an environmentally relevant mixture of 10 high molecular weight polycyclic aromatic hydrocarbons (PAHs) at a dose of 0.66 or 7.82 µg PAH · g fish(-1) · d(-1). At 3, 7, 14, and 28 d, biomarkers of aryl hydrocarbon receptor activation (AHR), hepatic microsomal ethoxyresorufin-O-deethylase (EROD) activity, and cytochrome P4501A (CYP1A)-associated staining increased 14- to 26-fold and 6- to 14-fold, respectively, in fish fed 7.82 µg PAH · g fish (-1) · d(-1). Cytochrome P4501A-associated staining increased 2- to 9-fold on days 3, 7, and 28 in fish fed 0.66 µg PAH · g fish(-1) · d(-1). Bile fluorescent aromatic compounds served as a biomarker of exposure and confirmed that PAH exposure was consistent over 50 d. DNA damage in blood cells, protein oxidation, and lipid peroxidation in the kidney were biomarkers of oxidative stress and all increased in fish fed 7.82 µg PAH · g fish(-1) · d(-1). Fish fed 0.66 µg PAH · g fish(-1) · d(-1) had elevated DNA damage in blood cells but increased protein oxidation or lipid peroxidation in the kidney were not observed. Challenge with Aeromonas salmonicida, at lethal concentration (LC) 20, decreased survival in fish previously fed either 0.66 µg PAH · g fish(-1) · d(-1) or 7.82 µg PAH · g fish(-1) · d(-1) relative to fish fed the control diet. In general, biomarkers of both AHR activation and oxidative stress peaked at 3 to 14 d then declined at 28 to 50 d of PAH exposure and an increase in susceptibility to disease was observed at 50 d. These results link PAH exposure to biomarker responses that may be useful as early indicators of population level responses, such as mortality resulting from an increase in disease susceptibility.

show abstract

Immunohistochemical Localization of CYP1A-Like and CYP3A-like Isozymes in Hepatic and Extrahepatic Tissues of Atlantic Cod (Gadus morhua L), a Marine Fish

Cited by 112 publications

References 0 publications

beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

beta-Naphthoflavone abolishes interrenal sensitivity to ACTH stimulation in rainbow trout

Aflatoxins in aquatic species: metabolism, toxicity and perspectives

Biomarker responses and disease susceptibility in juvenile rainbow trout Oncorhynchus mykiss fed a high molecular weight PAH mixture

Contact Info

Product

Resources

About